Strain Fitness in Escherichia coli BW25113 around b1723

Experiment: D-Glucosamine Hydrochloride (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntydiY and pfkB are separated by 286 nucleotidespfkB and ydiZ are separated by 100 nucleotidesydiZ and yniA are separated by 105 nucleotides b1722: ydiY - hypothetical protein (NCBI), at 1,803,349 to 1,804,107 ydiY b1723: pfkB - 6-phosphofructokinase II; suppressor of pfkA (VIMSS), at 1,804,394 to 1,805,323 pfkB b1724: ydiZ - hypothetical protein (NCBI), at 1,805,424 to 1,805,714 ydiZ b1725: yniA - predicted phosphotransferase/kinase (NCBI), at 1,805,820 to 1,806,680 yniA Position (kb) 1804 1805 1806Strain fitness (log2 ratio) -2 -1 0 1at 1803.483 kb on + strand, within ydiYat 1803.550 kb on + strand, within ydiYat 1803.588 kb on - strand, within ydiYat 1803.589 kb on - strand, within ydiYat 1803.613 kb on + strand, within ydiYat 1803.642 kb on - strand, within ydiYat 1803.644 kb on - strand, within ydiYat 1803.669 kb on - strand, within ydiYat 1803.672 kb on - strand, within ydiYat 1803.672 kb on - strand, within ydiYat 1803.870 kb on - strand, within ydiYat 1803.889 kb on - strand, within ydiYat 1803.889 kb on - strand, within ydiYat 1803.889 kb on - strand, within ydiYat 1803.889 kb on - strand, within ydiYat 1803.896 kb on - strand, within ydiYat 1803.938 kb on + strand, within ydiYat 1803.997 kb on + strand, within ydiYat 1803.997 kb on + strand, within ydiYat 1804.005 kb on - strand, within ydiYat 1804.005 kb on - strand, within ydiYat 1804.006 kb on + strand, within ydiYat 1804.014 kb on - strand, within ydiYat 1804.014 kb on - strand, within ydiYat 1804.089 kb on + strandat 1804.413 kb on - strandat 1804.413 kb on - strandat 1804.490 kb on - strand, within pfkBat 1804.501 kb on - strand, within pfkBat 1804.635 kb on + strand, within pfkBat 1804.635 kb on + strand, within pfkBat 1804.785 kb on + strand, within pfkBat 1804.911 kb on + strand, within pfkBat 1804.911 kb on + strand, within pfkBat 1804.911 kb on + strand, within pfkBat 1805.063 kb on + strand, within pfkBat 1805.063 kb on + strand, within pfkBat 1805.077 kb on - strand, within pfkBat 1805.136 kb on - strand, within pfkBat 1805.145 kb on + strand, within pfkBat 1805.355 kb on + strandat 1805.471 kb on - strand, within ydiZat 1805.648 kb on - strand, within ydiZat 1805.853 kb on + strandat 1805.863 kb on - strandat 1805.863 kb on - strandat 1805.980 kb on - strand, within yniAat 1806.165 kb on - strand, within yniAat 1806.217 kb on - strand, within yniA

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride (C)
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1,803,483 + ydiY b1722 0.18 +0.9
1,803,550 + ydiY b1722 0.26 +0.0
1,803,588 - ydiY b1722 0.31 -1.8
1,803,589 - ydiY b1722 0.32 -1.2
1,803,613 + ydiY b1722 0.35 +0.5
1,803,642 - ydiY b1722 0.39 +0.5
1,803,644 - ydiY b1722 0.39 +1.2
1,803,669 - ydiY b1722 0.42 -1.0
1,803,672 - ydiY b1722 0.43 +0.5
1,803,672 - ydiY b1722 0.43 -0.7
1,803,870 - ydiY b1722 0.69 -0.1
1,803,889 - ydiY b1722 0.71 +0.3
1,803,889 - ydiY b1722 0.71 +0.6
1,803,889 - ydiY b1722 0.71 -0.1
1,803,889 - ydiY b1722 0.71 -0.1
1,803,896 - ydiY b1722 0.72 +0.8
1,803,938 + ydiY b1722 0.78 +0.1
1,803,997 + ydiY b1722 0.85 -0.2
1,803,997 + ydiY b1722 0.85 -0.2
1,804,005 - ydiY b1722 0.86 +1.0
1,804,005 - ydiY b1722 0.86 +1.1
1,804,006 + ydiY b1722 0.87 -1.2
1,804,014 - ydiY b1722 0.88 +1.7
1,804,014 - ydiY b1722 0.88 +0.3
1,804,089 + -1.1
1,804,413 - +0.3
1,804,413 - -0.1
1,804,490 - pfkB b1723 0.10 +0.4
1,804,501 - pfkB b1723 0.12 -0.7
1,804,635 + pfkB b1723 0.26 -0.2
1,804,635 + pfkB b1723 0.26 -0.6
1,804,785 + pfkB b1723 0.42 -0.4
1,804,911 + pfkB b1723 0.56 +0.7
1,804,911 + pfkB b1723 0.56 -0.6
1,804,911 + pfkB b1723 0.56 -0.6
1,805,063 + pfkB b1723 0.72 +0.8
1,805,063 + pfkB b1723 0.72 +0.8
1,805,077 - pfkB b1723 0.73 -0.3
1,805,136 - pfkB b1723 0.80 +0.3
1,805,145 + pfkB b1723 0.81 -1.3
1,805,355 + -2.3
1,805,471 - ydiZ b1724 0.16 -1.5
1,805,648 - ydiZ b1724 0.77 -1.2
1,805,853 + +1.3
1,805,863 - -0.2
1,805,863 - -0.9
1,805,980 - yniA b1725 0.19 +0.1
1,806,165 - yniA b1725 0.40 +0.8
1,806,217 - yniA b1725 0.46 +0.1

Or see this region's nucleotide sequence