Experiment: Cobalt chloride 80 uM, aerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI46_12550 and IAI46_12555 overlap by 10 nucleotides IAI46_12555 and IAI46_12560 overlap by 17 nucleotides IAI46_12560 and IAI46_12565 overlap by 4 nucleotides
IAI46_12550: IAI46_12550 - hydrogenase nickel incorporation protein HypB, at 2,667,387 to 2,668,181
_12550
IAI46_12555: IAI46_12555 - HypC/HybG/HupF family hydrogenase formation chaperone, at 2,668,172 to 2,668,468
_12555
IAI46_12560: IAI46_12560 - hydrogenase formation protein HypD, at 2,668,452 to 2,669,579
_12560
IAI46_12565: IAI46_12565 - hydrogenase expression/formation protein HypE, at 2,669,576 to 2,670,589
_12565
Position (kb)
2668
2669
2670 Strain fitness (log2 ratio)
-1
0
1
2 at 2667.756 kb on - strand, within IAI46_12550 at 2667.989 kb on + strand, within IAI46_12550 at 2667.989 kb on + strand, within IAI46_12550 at 2667.990 kb on - strand, within IAI46_12550 at 2668.041 kb on + strand, within IAI46_12550 at 2668.042 kb on - strand, within IAI46_12550 at 2668.106 kb on - strand at 2668.171 kb on + strand at 2668.172 kb on - strand at 2668.172 kb on - strand at 2668.172 kb on - strand at 2668.179 kb on + strand at 2668.179 kb on + strand at 2668.378 kb on + strand, within IAI46_12555 at 2668.458 kb on + strand at 2668.462 kb on + strand at 2668.463 kb on - strand at 2668.508 kb on + strand at 2668.509 kb on - strand at 2668.509 kb on - strand at 2668.963 kb on + strand, within IAI46_12560 at 2669.023 kb on + strand, within IAI46_12560 at 2669.328 kb on - strand, within IAI46_12560 at 2669.328 kb on - strand, within IAI46_12560 at 2669.590 kb on + strand at 2669.590 kb on + strand at 2669.593 kb on + strand at 2669.756 kb on + strand, within IAI46_12565 at 2669.793 kb on + strand, within IAI46_12565 at 2669.872 kb on + strand, within IAI46_12565 at 2669.904 kb on + strand, within IAI46_12565 at 2669.950 kb on + strand, within IAI46_12565 at 2670.124 kb on + strand, within IAI46_12565 at 2670.151 kb on + strand, within IAI46_12565 at 2670.151 kb on + strand, within IAI46_12565 at 2670.152 kb on - strand, within IAI46_12565 at 2670.513 kb on + strand at 2670.513 kb on + strand at 2670.530 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 80 uM, aerobic remove 2,667,756 - IAI46_12550 0.46 -0.5 2,667,989 + IAI46_12550 0.76 -0.1 2,667,989 + IAI46_12550 0.76 -0.3 2,667,990 - IAI46_12550 0.76 -0.1 2,668,041 + IAI46_12550 0.82 +0.0 2,668,042 - IAI46_12550 0.82 +0.2 2,668,106 - +1.3 2,668,171 + +0.7 2,668,172 - -0.1 2,668,172 - -0.5 2,668,172 - +0.0 2,668,179 + +0.3 2,668,179 + -0.1 2,668,378 + IAI46_12555 0.69 +1.1 2,668,458 + +0.9 2,668,462 + -0.1 2,668,463 - -0.7 2,668,508 + +1.3 2,668,509 - +2.1 2,668,509 - -1.7 2,668,963 + IAI46_12560 0.45 +0.9 2,669,023 + IAI46_12560 0.51 -0.1 2,669,328 - IAI46_12560 0.78 +0.0 2,669,328 - IAI46_12560 0.78 +1.2 2,669,590 + -0.2 2,669,590 + +1.1 2,669,593 + +1.0 2,669,756 + IAI46_12565 0.18 +0.4 2,669,793 + IAI46_12565 0.21 +0.2 2,669,872 + IAI46_12565 0.29 -0.5 2,669,904 + IAI46_12565 0.32 +0.9 2,669,950 + IAI46_12565 0.37 -0.4 2,670,124 + IAI46_12565 0.54 +0.4 2,670,151 + IAI46_12565 0.57 +0.5 2,670,151 + IAI46_12565 0.57 -0.1 2,670,152 - IAI46_12565 0.57 +0.6 2,670,513 + +0.6 2,670,513 + +0.9 2,670,530 + +0.2
Or see this region's nucleotide sequence