Experiment: Cobalt chloride 80 uM, aerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI46_09295 and IAI46_09300 overlap by 4 nucleotides IAI46_09300 and IAI46_09305 are separated by 21 nucleotides IAI46_09305 and IAI46_09310 overlap by 4 nucleotides IAI46_09310 and IAI46_09315 overlap by 4 nucleotides IAI46_09315 and IAI46_09320 are separated by 144 nucleotides IAI46_09320 and IAI46_09325 are separated by 39 nucleotides
IAI46_09295: IAI46_09295 - tyrosine-type recombinase/integrase, at 1,990,112 to 1,991,278
_09295
IAI46_09300: IAI46_09300 - AlpA family phage regulatory protein, at 1,991,275 to 1,991,463
_09300
IAI46_09305: IAI46_09305 - hypothetical protein, at 1,991,485 to 1,991,670
_09305
IAI46_09310: IAI46_09310 - hypothetical protein, at 1,991,667 to 1,991,948
_09310
IAI46_09315: IAI46_09315 - DNA methyltransferase, at 1,991,945 to 1,992,580
_09315
IAI46_09320: IAI46_09320 - AlpA family phage regulatory protein, at 1,992,725 to 1,992,976
_09320
IAI46_09325: IAI46_09325 - recombinase RecT, at 1,993,016 to 1,993,900
_09325
Position (kb)
1991
1992
1993 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1991.066 kb on + strand, within IAI46_09295 at 1991.066 kb on + strand, within IAI46_09295 at 1991.067 kb on - strand, within IAI46_09295 at 1991.067 kb on - strand, within IAI46_09295 at 1991.068 kb on + strand, within IAI46_09295 at 1991.068 kb on + strand, within IAI46_09295 at 1991.082 kb on + strand, within IAI46_09295 at 1991.083 kb on - strand, within IAI46_09295 at 1991.118 kb on + strand, within IAI46_09295 at 1991.125 kb on + strand, within IAI46_09295 at 1991.160 kb on + strand, within IAI46_09295 at 1991.160 kb on + strand, within IAI46_09295 at 1991.160 kb on + strand, within IAI46_09295 at 1991.173 kb on + strand at 1991.184 kb on + strand at 1991.277 kb on - strand at 1991.280 kb on + strand at 1991.381 kb on + strand, within IAI46_09300 at 1991.385 kb on + strand, within IAI46_09300 at 1991.425 kb on + strand, within IAI46_09300 at 1991.430 kb on + strand, within IAI46_09300 at 1991.436 kb on + strand, within IAI46_09300 at 1991.464 kb on - strand at 1991.478 kb on + strand at 1991.482 kb on + strand at 1991.482 kb on + strand at 1991.482 kb on + strand at 1991.483 kb on - strand at 1991.483 kb on - strand at 1991.514 kb on + strand, within IAI46_09305 at 1991.614 kb on + strand, within IAI46_09305 at 1991.615 kb on - strand, within IAI46_09305 at 1991.615 kb on - strand, within IAI46_09305 at 1991.615 kb on - strand, within IAI46_09305 at 1991.615 kb on - strand, within IAI46_09305 at 1991.633 kb on - strand, within IAI46_09305 at 1991.659 kb on + strand at 1991.659 kb on + strand at 1991.660 kb on - strand at 1991.660 kb on - strand at 1991.682 kb on + strand at 1991.682 kb on + strand at 1991.758 kb on + strand, within IAI46_09310 at 1991.759 kb on - strand, within IAI46_09310 at 1991.759 kb on - strand, within IAI46_09310 at 1991.796 kb on + strand, within IAI46_09310 at 1991.796 kb on + strand, within IAI46_09310 at 1991.922 kb on + strand at 1991.923 kb on - strand at 1991.924 kb on + strand at 1991.924 kb on + strand at 1991.925 kb on - strand at 1992.087 kb on + strand, within IAI46_09315 at 1992.089 kb on + strand, within IAI46_09315 at 1992.090 kb on - strand, within IAI46_09315 at 1992.139 kb on + strand, within IAI46_09315 at 1992.237 kb on + strand, within IAI46_09315 at 1992.238 kb on - strand, within IAI46_09315 at 1992.439 kb on - strand, within IAI46_09315 at 1992.439 kb on - strand, within IAI46_09315 at 1992.439 kb on - strand, within IAI46_09315 at 1992.445 kb on + strand, within IAI46_09315 at 1992.445 kb on + strand, within IAI46_09315 at 1992.446 kb on - strand, within IAI46_09315 at 1992.446 kb on - strand, within IAI46_09315 at 1992.494 kb on + strand, within IAI46_09315 at 1992.559 kb on - strand at 1992.582 kb on - strand at 1992.582 kb on - strand at 1992.591 kb on + strand at 1992.592 kb on - strand at 1992.592 kb on - strand at 1992.626 kb on + strand at 1992.754 kb on - strand, within IAI46_09320 at 1992.818 kb on - strand, within IAI46_09320 at 1992.818 kb on - strand, within IAI46_09320 at 1992.914 kb on - strand, within IAI46_09320 at 1992.963 kb on - strand at 1992.963 kb on - strand at 1992.981 kb on - strand at 1992.981 kb on - strand at 1993.018 kb on - strand at 1993.021 kb on + strand at 1993.022 kb on - strand at 1993.114 kb on - strand, within IAI46_09325 at 1993.117 kb on + strand, within IAI46_09325 at 1993.117 kb on + strand, within IAI46_09325 at 1993.118 kb on - strand, within IAI46_09325 at 1993.168 kb on + strand, within IAI46_09325 at 1993.338 kb on + strand, within IAI46_09325 at 1993.339 kb on - strand, within IAI46_09325 at 1993.365 kb on + strand, within IAI46_09325 at 1993.380 kb on + strand, within IAI46_09325 at 1993.561 kb on - strand, within IAI46_09325
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 80 uM, aerobic remove 1,991,066 + IAI46_09295 0.82 +1.2 1,991,066 + IAI46_09295 0.82 +0.1 1,991,067 - IAI46_09295 0.82 -0.1 1,991,067 - IAI46_09295 0.82 -0.3 1,991,068 + IAI46_09295 0.82 -0.4 1,991,068 + IAI46_09295 0.82 +0.4 1,991,082 + IAI46_09295 0.83 +1.8 1,991,083 - IAI46_09295 0.83 -0.3 1,991,118 + IAI46_09295 0.86 +0.5 1,991,125 + IAI46_09295 0.87 +0.6 1,991,160 + IAI46_09295 0.90 -0.1 1,991,160 + IAI46_09295 0.90 -0.3 1,991,160 + IAI46_09295 0.90 +1.0 1,991,173 + +1.1 1,991,184 + +2.1 1,991,277 - -0.5 1,991,280 + -0.0 1,991,381 + IAI46_09300 0.56 +0.7 1,991,385 + IAI46_09300 0.58 +0.3 1,991,425 + IAI46_09300 0.79 -0.6 1,991,430 + IAI46_09300 0.82 +0.5 1,991,436 + IAI46_09300 0.85 -1.4 1,991,464 - +1.0 1,991,478 + +0.0 1,991,482 + +1.5 1,991,482 + +0.5 1,991,482 + +0.0 1,991,483 - -1.2 1,991,483 - +0.3 1,991,514 + IAI46_09305 0.16 +0.4 1,991,614 + IAI46_09305 0.69 -0.9 1,991,615 - IAI46_09305 0.70 +0.2 1,991,615 - IAI46_09305 0.70 +0.2 1,991,615 - IAI46_09305 0.70 +1.3 1,991,615 - IAI46_09305 0.70 +0.7 1,991,633 - IAI46_09305 0.80 -1.9 1,991,659 + -0.3 1,991,659 + -0.4 1,991,660 - +0.5 1,991,660 - -1.0 1,991,682 + +0.4 1,991,682 + -0.2 1,991,758 + IAI46_09310 0.32 -0.4 1,991,759 - IAI46_09310 0.33 +0.1 1,991,759 - IAI46_09310 0.33 +0.5 1,991,796 + IAI46_09310 0.46 -0.0 1,991,796 + IAI46_09310 0.46 -0.3 1,991,922 + +0.2 1,991,923 - -0.9 1,991,924 + +0.9 1,991,924 + -0.1 1,991,925 - +0.0 1,992,087 + IAI46_09315 0.22 +0.6 1,992,089 + IAI46_09315 0.23 -0.1 1,992,090 - IAI46_09315 0.23 +0.1 1,992,139 + IAI46_09315 0.31 -0.1 1,992,237 + IAI46_09315 0.46 -0.1 1,992,238 - IAI46_09315 0.46 +0.3 1,992,439 - IAI46_09315 0.78 +1.1 1,992,439 - IAI46_09315 0.78 +0.5 1,992,439 - IAI46_09315 0.78 +0.3 1,992,445 + IAI46_09315 0.79 +0.1 1,992,445 + IAI46_09315 0.79 +0.0 1,992,446 - IAI46_09315 0.79 -0.2 1,992,446 - IAI46_09315 0.79 +0.2 1,992,494 + IAI46_09315 0.86 +1.2 1,992,559 - +0.6 1,992,582 - -0.0 1,992,582 - -0.4 1,992,591 + +0.6 1,992,592 - +0.1 1,992,592 - +0.9 1,992,626 + -0.1 1,992,754 - IAI46_09320 0.12 +0.7 1,992,818 - IAI46_09320 0.37 +0.1 1,992,818 - IAI46_09320 0.37 +0.9 1,992,914 - IAI46_09320 0.75 -0.1 1,992,963 - -0.4 1,992,963 - +1.0 1,992,981 - -0.3 1,992,981 - -0.6 1,993,018 - +0.5 1,993,021 + +0.1 1,993,022 - +1.3 1,993,114 - IAI46_09325 0.11 -0.1 1,993,117 + IAI46_09325 0.11 +0.6 1,993,117 + IAI46_09325 0.11 +0.1 1,993,118 - IAI46_09325 0.12 +1.5 1,993,168 + IAI46_09325 0.17 +0.4 1,993,338 + IAI46_09325 0.36 +0.3 1,993,339 - IAI46_09325 0.36 +0.9 1,993,365 + IAI46_09325 0.39 -0.1 1,993,380 + IAI46_09325 0.41 -0.2 1,993,561 - IAI46_09325 0.62 +0.3
Or see this region's nucleotide sequence