Experiment: Cobalt chloride 80 uM, aerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI46_07075 and IAI46_07080 are separated by 20 nucleotides IAI46_07080 and IAI46_07085 overlap by 8 nucleotides IAI46_07085 and IAI46_07090 are separated by 251 nucleotides IAI46_07090 and IAI46_07095 are separated by 29 nucleotides
IAI46_07075: IAI46_07075 - methionine ABC transporter substrate-binding protein, at 1,494,496 to 1,495,314
_07075
IAI46_07080: IAI46_07080 - ABC transporter permease, at 1,495,335 to 1,496,003
_07080
IAI46_07085: IAI46_07085 - methionine ABC transporter ATP-binding protein, at 1,495,996 to 1,497,021
_07085
IAI46_07090: IAI46_07090 - acetyltransferase, at 1,497,273 to 1,497,716
_07090
IAI46_07095: IAI46_07095 - LysR family transcriptional regulator, at 1,497,746 to 1,498,636
_07095
Position (kb)
1495
1496
1497
1498 Strain fitness (log2 ratio)
-2
-1
0
1 at 1495.022 kb on + strand, within IAI46_07075 at 1495.022 kb on + strand, within IAI46_07075 at 1495.022 kb on + strand, within IAI46_07075 at 1495.047 kb on + strand, within IAI46_07075 at 1495.065 kb on - strand, within IAI46_07075 at 1495.131 kb on - strand, within IAI46_07075 at 1495.160 kb on - strand, within IAI46_07075 at 1495.218 kb on + strand, within IAI46_07075 at 1495.219 kb on - strand, within IAI46_07075 at 1495.219 kb on - strand, within IAI46_07075 at 1495.219 kb on - strand, within IAI46_07075 at 1495.299 kb on + strand at 1495.300 kb on - strand at 1495.300 kb on - strand at 1495.300 kb on - strand at 1495.321 kb on + strand at 1495.333 kb on - strand at 1495.337 kb on - strand at 1495.358 kb on + strand at 1495.359 kb on - strand at 1495.359 kb on - strand at 1495.398 kb on + strand at 1495.398 kb on + strand at 1495.399 kb on - strand at 1495.399 kb on - strand at 1495.533 kb on - strand, within IAI46_07080 at 1495.589 kb on + strand, within IAI46_07080 at 1495.679 kb on - strand, within IAI46_07080 at 1495.832 kb on - strand, within IAI46_07080 at 1495.903 kb on - strand, within IAI46_07080 at 1495.934 kb on + strand, within IAI46_07080 at 1495.934 kb on + strand, within IAI46_07080 at 1495.935 kb on - strand, within IAI46_07080 at 1495.935 kb on - strand, within IAI46_07080 at 1495.935 kb on - strand, within IAI46_07080 at 1495.936 kb on + strand, within IAI46_07080 at 1495.936 kb on + strand, within IAI46_07080 at 1495.937 kb on - strand at 1495.937 kb on - strand at 1495.937 kb on - strand at 1496.008 kb on - strand at 1496.090 kb on + strand at 1496.091 kb on - strand at 1496.148 kb on + strand, within IAI46_07085 at 1496.148 kb on + strand, within IAI46_07085 at 1496.148 kb on + strand, within IAI46_07085 at 1496.148 kb on + strand, within IAI46_07085 at 1496.149 kb on - strand, within IAI46_07085 at 1496.149 kb on - strand, within IAI46_07085 at 1496.149 kb on - strand, within IAI46_07085 at 1496.149 kb on - strand, within IAI46_07085 at 1496.152 kb on + strand, within IAI46_07085 at 1496.152 kb on + strand, within IAI46_07085 at 1496.153 kb on - strand, within IAI46_07085 at 1496.160 kb on - strand, within IAI46_07085 at 1496.160 kb on - strand, within IAI46_07085 at 1496.612 kb on + strand, within IAI46_07085 at 1497.038 kb on - strand at 1497.041 kb on + strand at 1497.042 kb on - strand at 1497.042 kb on - strand at 1497.044 kb on + strand at 1497.044 kb on + strand at 1497.045 kb on - strand at 1497.045 kb on - strand at 1497.158 kb on + strand at 1497.204 kb on + strand at 1497.521 kb on + strand, within IAI46_07090 at 1497.580 kb on + strand, within IAI46_07090 at 1497.581 kb on - strand, within IAI46_07090 at 1497.581 kb on - strand, within IAI46_07090 at 1497.719 kb on + strand at 1497.720 kb on - strand at 1497.743 kb on - strand at 1497.770 kb on + strand at 1497.825 kb on + strand at 1497.825 kb on + strand at 1497.826 kb on - strand at 1497.826 kb on - strand at 1497.826 kb on - strand at 1497.858 kb on + strand, within IAI46_07095 at 1497.858 kb on + strand, within IAI46_07095
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 80 uM, aerobic remove 1,495,022 + IAI46_07075 0.64 -0.3 1,495,022 + IAI46_07075 0.64 -0.9 1,495,022 + IAI46_07075 0.64 +0.1 1,495,047 + IAI46_07075 0.67 +0.0 1,495,065 - IAI46_07075 0.69 -1.3 1,495,131 - IAI46_07075 0.78 -0.0 1,495,160 - IAI46_07075 0.81 -0.2 1,495,218 + IAI46_07075 0.88 +0.5 1,495,219 - IAI46_07075 0.88 +0.5 1,495,219 - IAI46_07075 0.88 -0.0 1,495,219 - IAI46_07075 0.88 +0.1 1,495,299 + -1.2 1,495,300 - +1.4 1,495,300 - +0.3 1,495,300 - -0.7 1,495,321 + +1.0 1,495,333 - -0.1 1,495,337 - +0.3 1,495,358 + +0.1 1,495,359 - +0.5 1,495,359 - +0.7 1,495,398 + -1.2 1,495,398 + -0.0 1,495,399 - -0.2 1,495,399 - +0.1 1,495,533 - IAI46_07080 0.30 +0.2 1,495,589 + IAI46_07080 0.38 -1.2 1,495,679 - IAI46_07080 0.51 +0.5 1,495,832 - IAI46_07080 0.74 -0.2 1,495,903 - IAI46_07080 0.85 +0.9 1,495,934 + IAI46_07080 0.90 -0.9 1,495,934 + IAI46_07080 0.90 -0.8 1,495,935 - IAI46_07080 0.90 +1.1 1,495,935 - IAI46_07080 0.90 -0.4 1,495,935 - IAI46_07080 0.90 -0.0 1,495,936 + IAI46_07080 0.90 +0.3 1,495,936 + IAI46_07080 0.90 +0.3 1,495,937 - +0.1 1,495,937 - +0.7 1,495,937 - -0.5 1,496,008 - -0.4 1,496,090 + -0.5 1,496,091 - +0.3 1,496,148 + IAI46_07085 0.15 -0.5 1,496,148 + IAI46_07085 0.15 +0.5 1,496,148 + IAI46_07085 0.15 +0.6 1,496,148 + IAI46_07085 0.15 +0.1 1,496,149 - IAI46_07085 0.15 -0.0 1,496,149 - IAI46_07085 0.15 -0.6 1,496,149 - IAI46_07085 0.15 +0.0 1,496,149 - IAI46_07085 0.15 +0.5 1,496,152 + IAI46_07085 0.15 -0.1 1,496,152 + IAI46_07085 0.15 -0.1 1,496,153 - IAI46_07085 0.15 +0.1 1,496,160 - IAI46_07085 0.16 -0.3 1,496,160 - IAI46_07085 0.16 +1.1 1,496,612 + IAI46_07085 0.60 -0.4 1,497,038 - +0.8 1,497,041 + -0.2 1,497,042 - -0.8 1,497,042 - -0.6 1,497,044 + -2.6 1,497,044 + -1.4 1,497,045 - -0.3 1,497,045 - +0.3 1,497,158 + +0.6 1,497,204 + +0.9 1,497,521 + IAI46_07090 0.56 -0.7 1,497,580 + IAI46_07090 0.69 -1.0 1,497,581 - IAI46_07090 0.69 -1.6 1,497,581 - IAI46_07090 0.69 +0.5 1,497,719 + +0.3 1,497,720 - -0.2 1,497,743 - -0.8 1,497,770 + +0.4 1,497,825 + +0.3 1,497,825 + -0.3 1,497,826 - +0.4 1,497,826 - +0.2 1,497,826 - +0.3 1,497,858 + IAI46_07095 0.13 -1.0 1,497,858 + IAI46_07095 0.13 -0.7
Or see this region's nucleotide sequence