Experiment: Cobalt chloride 80 uM, aerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI46_03730 and IAI46_03735 are separated by 52 nucleotides IAI46_03735 and IAI46_03740 overlap by 17 nucleotides IAI46_03740 and IAI46_03745 are separated by 101 nucleotides
IAI46_03730: IAI46_03730 - DUF3561 family protein, at 813,801 to 814,118
_03730
IAI46_03735: IAI46_03735 - amino acid ABC transporter ATP-binding protein, at 814,171 to 814,893
_03735
IAI46_03740: IAI46_03740 - amino acid ABC transporter permease, at 814,877 to 815,596
_03740
IAI46_03745: IAI46_03745 - basic amino acid ABC transporter substrate-binding protein, at 815,698 to 816,468
_03745
Position (kb)
814
815
816 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 813.957 kb on + strand, within IAI46_03730 at 813.957 kb on + strand, within IAI46_03730 at 813.958 kb on - strand, within IAI46_03730 at 813.958 kb on - strand, within IAI46_03730 at 814.047 kb on - strand, within IAI46_03730 at 814.268 kb on + strand, within IAI46_03735 at 814.552 kb on + strand, within IAI46_03735 at 814.553 kb on - strand, within IAI46_03735 at 814.636 kb on - strand, within IAI46_03735 at 814.770 kb on + strand, within IAI46_03735 at 814.777 kb on - strand, within IAI46_03735 at 814.777 kb on - strand, within IAI46_03735 at 814.878 kb on + strand at 814.878 kb on + strand at 814.879 kb on - strand at 814.882 kb on + strand at 814.883 kb on - strand at 814.883 kb on - strand at 814.957 kb on - strand, within IAI46_03740 at 814.957 kb on - strand, within IAI46_03740 at 814.989 kb on + strand, within IAI46_03740 at 814.990 kb on - strand, within IAI46_03740 at 814.998 kb on + strand, within IAI46_03740 at 814.998 kb on + strand, within IAI46_03740 at 815.203 kb on + strand, within IAI46_03740 at 815.203 kb on + strand, within IAI46_03740 at 815.238 kb on + strand, within IAI46_03740 at 815.238 kb on + strand, within IAI46_03740 at 815.238 kb on + strand, within IAI46_03740 at 815.238 kb on + strand, within IAI46_03740 at 815.238 kb on + strand, within IAI46_03740 at 815.238 kb on + strand, within IAI46_03740 at 815.239 kb on - strand, within IAI46_03740 at 815.239 kb on - strand, within IAI46_03740 at 815.239 kb on - strand, within IAI46_03740 at 815.258 kb on - strand, within IAI46_03740 at 815.258 kb on - strand, within IAI46_03740 at 815.365 kb on + strand, within IAI46_03740 at 815.366 kb on - strand, within IAI46_03740 at 815.419 kb on + strand, within IAI46_03740 at 815.439 kb on + strand, within IAI46_03740 at 815.439 kb on + strand, within IAI46_03740 at 815.439 kb on + strand, within IAI46_03740 at 815.440 kb on - strand, within IAI46_03740 at 815.440 kb on - strand, within IAI46_03740 at 815.440 kb on - strand, within IAI46_03740 at 815.440 kb on - strand, within IAI46_03740 at 815.466 kb on + strand, within IAI46_03740 at 815.560 kb on + strand at 815.560 kb on + strand at 815.560 kb on + strand at 815.561 kb on - strand at 815.561 kb on - strand at 815.562 kb on - strand at 815.622 kb on - strand at 815.622 kb on - strand at 815.622 kb on - strand at 815.656 kb on + strand at 815.699 kb on + strand at 815.745 kb on - strand at 815.756 kb on + strand at 815.756 kb on + strand at 815.844 kb on - strand, within IAI46_03745 at 815.904 kb on + strand, within IAI46_03745 at 815.906 kb on + strand, within IAI46_03745 at 815.906 kb on + strand, within IAI46_03745 at 815.907 kb on - strand, within IAI46_03745 at 815.907 kb on - strand, within IAI46_03745 at 815.907 kb on - strand, within IAI46_03745 at 815.907 kb on - strand, within IAI46_03745 at 815.947 kb on - strand, within IAI46_03745 at 815.957 kb on + strand, within IAI46_03745 at 815.983 kb on - strand, within IAI46_03745 at 816.020 kb on - strand, within IAI46_03745 at 816.062 kb on - strand, within IAI46_03745 at 816.062 kb on - strand, within IAI46_03745 at 816.244 kb on - strand, within IAI46_03745 at 816.259 kb on - strand, within IAI46_03745 at 816.310 kb on - strand, within IAI46_03745 at 816.314 kb on - strand, within IAI46_03745 at 816.316 kb on - strand, within IAI46_03745 at 816.354 kb on - strand, within IAI46_03745 at 816.365 kb on + strand, within IAI46_03745 at 816.365 kb on + strand, within IAI46_03745 at 816.366 kb on - strand, within IAI46_03745 at 816.389 kb on + strand, within IAI46_03745 at 816.389 kb on + strand, within IAI46_03745 at 816.389 kb on + strand, within IAI46_03745 at 816.389 kb on + strand, within IAI46_03745 at 816.389 kb on + strand, within IAI46_03745 at 816.390 kb on - strand, within IAI46_03745 at 816.393 kb on + strand at 816.393 kb on + strand at 816.393 kb on + strand at 816.394 kb on - strand at 816.454 kb on + strand at 816.463 kb on - strand at 816.516 kb on + strand at 816.561 kb on + strand at 816.590 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 80 uM, aerobic remove 813,957 + IAI46_03730 0.49 -0.7 813,957 + IAI46_03730 0.49 +0.5 813,958 - IAI46_03730 0.49 -0.3 813,958 - IAI46_03730 0.49 +0.0 814,047 - IAI46_03730 0.77 -0.1 814,268 + IAI46_03735 0.13 +2.0 814,552 + IAI46_03735 0.53 -0.3 814,553 - IAI46_03735 0.53 -0.7 814,636 - IAI46_03735 0.64 +0.9 814,770 + IAI46_03735 0.83 +0.2 814,777 - IAI46_03735 0.84 -0.9 814,777 - IAI46_03735 0.84 +1.2 814,878 + -0.5 814,878 + -1.5 814,879 - -1.2 814,882 + +0.1 814,883 - -0.7 814,883 - -0.2 814,957 - IAI46_03740 0.11 +0.3 814,957 - IAI46_03740 0.11 -0.5 814,989 + IAI46_03740 0.16 +1.2 814,990 - IAI46_03740 0.16 -0.9 814,998 + IAI46_03740 0.17 +0.4 814,998 + IAI46_03740 0.17 -0.0 815,203 + IAI46_03740 0.45 +1.3 815,203 + IAI46_03740 0.45 -0.4 815,238 + IAI46_03740 0.50 +0.5 815,238 + IAI46_03740 0.50 -0.7 815,238 + IAI46_03740 0.50 -1.1 815,238 + IAI46_03740 0.50 -0.3 815,238 + IAI46_03740 0.50 -0.6 815,238 + IAI46_03740 0.50 -0.0 815,239 - IAI46_03740 0.50 -0.4 815,239 - IAI46_03740 0.50 +0.6 815,239 - IAI46_03740 0.50 +0.9 815,258 - IAI46_03740 0.53 +0.2 815,258 - IAI46_03740 0.53 -0.4 815,365 + IAI46_03740 0.68 +0.2 815,366 - IAI46_03740 0.68 -0.1 815,419 + IAI46_03740 0.75 -0.0 815,439 + IAI46_03740 0.78 +0.2 815,439 + IAI46_03740 0.78 +1.0 815,439 + IAI46_03740 0.78 +0.4 815,440 - IAI46_03740 0.78 +1.7 815,440 - IAI46_03740 0.78 +0.1 815,440 - IAI46_03740 0.78 +0.7 815,440 - IAI46_03740 0.78 +2.6 815,466 + IAI46_03740 0.82 +0.5 815,560 + +0.0 815,560 + +0.7 815,560 + +0.3 815,561 - -1.8 815,561 - -0.1 815,562 - +0.5 815,622 - +0.4 815,622 - +0.4 815,622 - -0.4 815,656 + -0.3 815,699 + +0.9 815,745 - +0.4 815,756 + +0.2 815,756 + -0.8 815,844 - IAI46_03745 0.19 +0.4 815,904 + IAI46_03745 0.27 +1.1 815,906 + IAI46_03745 0.27 +0.7 815,906 + IAI46_03745 0.27 +0.1 815,907 - IAI46_03745 0.27 +0.4 815,907 - IAI46_03745 0.27 -0.4 815,907 - IAI46_03745 0.27 +2.0 815,907 - IAI46_03745 0.27 +0.1 815,947 - IAI46_03745 0.32 +0.5 815,957 + IAI46_03745 0.34 -0.0 815,983 - IAI46_03745 0.37 +0.2 816,020 - IAI46_03745 0.42 -0.9 816,062 - IAI46_03745 0.47 +0.2 816,062 - IAI46_03745 0.47 -0.7 816,244 - IAI46_03745 0.71 -0.0 816,259 - IAI46_03745 0.73 -0.0 816,310 - IAI46_03745 0.79 -0.4 816,314 - IAI46_03745 0.80 +0.0 816,316 - IAI46_03745 0.80 +1.0 816,354 - IAI46_03745 0.85 +0.6 816,365 + IAI46_03745 0.87 -0.7 816,365 + IAI46_03745 0.87 +1.2 816,366 - IAI46_03745 0.87 +0.8 816,389 + IAI46_03745 0.90 +0.4 816,389 + IAI46_03745 0.90 -0.8 816,389 + IAI46_03745 0.90 +0.6 816,389 + IAI46_03745 0.90 -0.5 816,389 + IAI46_03745 0.90 +0.4 816,390 - IAI46_03745 0.90 +0.3 816,393 + +0.0 816,393 + -0.6 816,393 + -0.1 816,394 - +0.6 816,454 + -0.6 816,463 - -0.6 816,516 + +0.7 816,561 + +0.4 816,590 - -0.2
Or see this region's nucleotide sequence