Experiment: 150_mag_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS20275 and AMB_RS20280 are separated by 58 nucleotides AMB_RS20280 and AMB_RS20285 are separated by 58 nucleotides AMB_RS20285 and AMB_RS20290 overlap by 26 nucleotides AMB_RS20290 and AMB_RS20295 are separated by 326 nucleotides
AMB_RS20275: AMB_RS20275 - glycerol-3-phosphate acyltransferase, at 4,413,501 to 4,414,491
_RS20275
AMB_RS20280: AMB_RS20280 - glutamate synthase, at 4,414,550 to 4,414,789
_RS20280
AMB_RS20285: AMB_RS20285 - methyl-accepting chemotaxis protein, at 4,414,848 to 4,416,146
_RS20285
AMB_RS20290: AMB_RS20290 - PAS domain S-box protein, at 4,416,121 to 4,416,645
_RS20290
AMB_RS20295: AMB_RS20295 - tryptophan synthase subunit beta, at 4,416,972 to 4,418,195
_RS20295
Position (kb)
4414
4415
4416
4417 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4414.614 kb on + strand, within AMB_RS20280 at 4414.615 kb on - strand, within AMB_RS20280 at 4414.615 kb on - strand, within AMB_RS20280 at 4414.615 kb on - strand, within AMB_RS20280 at 4414.639 kb on + strand, within AMB_RS20280 at 4414.639 kb on + strand, within AMB_RS20280 at 4414.641 kb on + strand, within AMB_RS20280 at 4414.641 kb on + strand, within AMB_RS20280 at 4414.642 kb on - strand, within AMB_RS20280 at 4414.642 kb on - strand, within AMB_RS20280 at 4414.725 kb on + strand, within AMB_RS20280 at 4414.725 kb on + strand, within AMB_RS20280 at 4414.725 kb on + strand, within AMB_RS20280 at 4414.726 kb on - strand, within AMB_RS20280 at 4414.726 kb on - strand, within AMB_RS20280 at 4414.726 kb on - strand, within AMB_RS20280 at 4414.726 kb on - strand, within AMB_RS20280 at 4414.819 kb on + strand at 4414.819 kb on + strand at 4414.819 kb on + strand at 4414.819 kb on + strand at 4414.819 kb on + strand at 4414.820 kb on - strand at 4414.820 kb on - strand at 4414.820 kb on - strand at 4414.820 kb on - strand at 4414.987 kb on + strand, within AMB_RS20285 at 4414.987 kb on + strand, within AMB_RS20285 at 4414.987 kb on + strand, within AMB_RS20285 at 4414.987 kb on + strand, within AMB_RS20285 at 4414.987 kb on + strand, within AMB_RS20285 at 4414.987 kb on + strand, within AMB_RS20285 at 4414.987 kb on + strand, within AMB_RS20285 at 4414.988 kb on - strand, within AMB_RS20285 at 4414.988 kb on - strand, within AMB_RS20285 at 4414.988 kb on - strand, within AMB_RS20285 at 4415.735 kb on - strand, within AMB_RS20285 at 4415.735 kb on - strand, within AMB_RS20285 at 4415.782 kb on + strand, within AMB_RS20285 at 4415.782 kb on + strand, within AMB_RS20285 at 4415.783 kb on - strand, within AMB_RS20285 at 4415.783 kb on - strand, within AMB_RS20285 at 4415.830 kb on + strand, within AMB_RS20285 at 4415.831 kb on - strand, within AMB_RS20285 at 4415.883 kb on - strand, within AMB_RS20285 at 4416.122 kb on + strand at 4416.122 kb on + strand at 4416.146 kb on + strand at 4416.251 kb on + strand, within AMB_RS20290 at 4416.251 kb on + strand, within AMB_RS20290 at 4416.252 kb on - strand, within AMB_RS20290 at 4416.252 kb on - strand, within AMB_RS20290 at 4416.365 kb on + strand, within AMB_RS20290 at 4416.398 kb on + strand, within AMB_RS20290 at 4416.398 kb on + strand, within AMB_RS20290 at 4416.398 kb on + strand, within AMB_RS20290 at 4416.399 kb on - strand, within AMB_RS20290 at 4416.399 kb on - strand, within AMB_RS20290 at 4416.399 kb on - strand, within AMB_RS20290 at 4416.548 kb on + strand, within AMB_RS20290 at 4416.548 kb on + strand, within AMB_RS20290 at 4416.548 kb on + strand, within AMB_RS20290 at 4416.549 kb on - strand, within AMB_RS20290 at 4416.640 kb on - strand at 4416.663 kb on - strand at 4416.669 kb on + strand at 4416.669 kb on + strand at 4416.669 kb on + strand at 4416.670 kb on - strand at 4416.696 kb on + strand at 4416.697 kb on - strand at 4416.697 kb on - strand at 4416.697 kb on - strand at 4416.697 kb on - strand at 4416.697 kb on - strand at 4416.931 kb on + strand at 4416.931 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 150_mag_3 remove 4,414,614 + AMB_RS20280 0.27 -1.7 4,414,615 - AMB_RS20280 0.27 +0.1 4,414,615 - AMB_RS20280 0.27 -0.1 4,414,615 - AMB_RS20280 0.27 -2.0 4,414,639 + AMB_RS20280 0.37 +1.0 4,414,639 + AMB_RS20280 0.37 +0.6 4,414,641 + AMB_RS20280 0.38 -0.4 4,414,641 + AMB_RS20280 0.38 -0.5 4,414,642 - AMB_RS20280 0.38 -0.7 4,414,642 - AMB_RS20280 0.38 -0.3 4,414,725 + AMB_RS20280 0.73 -0.4 4,414,725 + AMB_RS20280 0.73 +0.6 4,414,725 + AMB_RS20280 0.73 +0.2 4,414,726 - AMB_RS20280 0.73 -1.5 4,414,726 - AMB_RS20280 0.73 -0.9 4,414,726 - AMB_RS20280 0.73 +0.7 4,414,726 - AMB_RS20280 0.73 -0.8 4,414,819 + -2.9 4,414,819 + -0.2 4,414,819 + -0.1 4,414,819 + -0.5 4,414,819 + +0.2 4,414,820 - +0.3 4,414,820 - -0.3 4,414,820 - +0.5 4,414,820 - -1.5 4,414,987 + AMB_RS20285 0.11 +2.4 4,414,987 + AMB_RS20285 0.11 -0.1 4,414,987 + AMB_RS20285 0.11 -3.3 4,414,987 + AMB_RS20285 0.11 -0.5 4,414,987 + AMB_RS20285 0.11 -0.9 4,414,987 + AMB_RS20285 0.11 -0.5 4,414,987 + AMB_RS20285 0.11 -0.8 4,414,988 - AMB_RS20285 0.11 -0.9 4,414,988 - AMB_RS20285 0.11 +0.4 4,414,988 - AMB_RS20285 0.11 +1.7 4,415,735 - AMB_RS20285 0.68 +0.8 4,415,735 - AMB_RS20285 0.68 -0.1 4,415,782 + AMB_RS20285 0.72 -0.3 4,415,782 + AMB_RS20285 0.72 -1.7 4,415,783 - AMB_RS20285 0.72 -0.5 4,415,783 - AMB_RS20285 0.72 -1.3 4,415,830 + AMB_RS20285 0.76 +0.0 4,415,831 - AMB_RS20285 0.76 +0.4 4,415,883 - AMB_RS20285 0.80 -0.3 4,416,122 + -1.5 4,416,122 + -0.2 4,416,146 + +0.1 4,416,251 + AMB_RS20290 0.25 -0.5 4,416,251 + AMB_RS20290 0.25 +0.2 4,416,252 - AMB_RS20290 0.25 +0.1 4,416,252 - AMB_RS20290 0.25 -0.5 4,416,365 + AMB_RS20290 0.46 -0.1 4,416,398 + AMB_RS20290 0.53 -0.7 4,416,398 + AMB_RS20290 0.53 -1.1 4,416,398 + AMB_RS20290 0.53 -0.8 4,416,399 - AMB_RS20290 0.53 -1.8 4,416,399 - AMB_RS20290 0.53 -0.1 4,416,399 - AMB_RS20290 0.53 -0.8 4,416,548 + AMB_RS20290 0.81 -0.3 4,416,548 + AMB_RS20290 0.81 -0.4 4,416,548 + AMB_RS20290 0.81 +0.0 4,416,549 - AMB_RS20290 0.82 -0.4 4,416,640 - -0.6 4,416,663 - -1.5 4,416,669 + -0.3 4,416,669 + -0.2 4,416,669 + +1.3 4,416,670 - -0.3 4,416,696 + -0.3 4,416,697 - -0.5 4,416,697 - -0.0 4,416,697 - -0.4 4,416,697 - +0.3 4,416,697 - +0.3 4,416,931 + +1.3 4,416,931 + +0.6
Or see this region's nucleotide sequence