Experiment: 150_mag_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS17925 and AMB_RS17930 are separated by 2 nucleotides AMB_RS17930 and AMB_RS17935 are separated by 14 nucleotides AMB_RS17935 and AMB_RS17940 are separated by 45 nucleotides AMB_RS17940 and AMB_RS17945 are separated by 14 nucleotides AMB_RS17945 and AMB_RS17950 are separated by 305 nucleotides
AMB_RS17925: AMB_RS17925 - formate dehydrogenase, at 3,893,402 to 3,896,296
_RS17925
AMB_RS17930: AMB_RS17930 - 4Fe-4S dicluster domain-containing protein, at 3,896,299 to 3,897,036
_RS17930
AMB_RS17935: AMB_RS17935 - DMSO reductase, at 3,897,051 to 3,897,992
_RS17935
AMB_RS17940: AMB_RS17940 - hypothetical protein, at 3,898,038 to 3,898,238
_RS17940
AMB_RS17945: AMB_RS17945 - hypothetical protein, at 3,898,253 to 3,898,444
_RS17945
AMB_RS17950: AMB_RS17950 - hypothetical protein, at 3,898,750 to 3,899,178
_RS17950
Position (kb)
3897
3898 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3896.153 kb on + strand at 3896.470 kb on + strand, within AMB_RS17930 at 3896.470 kb on + strand, within AMB_RS17930 at 3896.470 kb on + strand, within AMB_RS17930 at 3896.470 kb on + strand, within AMB_RS17930 at 3896.471 kb on - strand, within AMB_RS17930 at 3896.471 kb on - strand, within AMB_RS17930 at 3896.471 kb on - strand, within AMB_RS17930 at 3896.575 kb on + strand, within AMB_RS17930 at 3896.575 kb on + strand, within AMB_RS17930 at 3896.575 kb on + strand, within AMB_RS17930 at 3896.576 kb on - strand, within AMB_RS17930 at 3896.576 kb on - strand, within AMB_RS17930 at 3896.576 kb on - strand, within AMB_RS17930 at 3896.576 kb on - strand, within AMB_RS17930 at 3896.576 kb on - strand, within AMB_RS17930 at 3896.576 kb on - strand, within AMB_RS17930 at 3896.576 kb on - strand, within AMB_RS17930 at 3896.611 kb on + strand, within AMB_RS17930 at 3896.612 kb on - strand, within AMB_RS17930 at 3896.635 kb on + strand, within AMB_RS17930 at 3896.635 kb on + strand, within AMB_RS17930 at 3896.636 kb on - strand, within AMB_RS17930 at 3896.636 kb on - strand, within AMB_RS17930 at 3896.636 kb on - strand, within AMB_RS17930 at 3896.636 kb on - strand, within AMB_RS17930 at 3896.725 kb on + strand, within AMB_RS17930 at 3896.725 kb on + strand, within AMB_RS17930 at 3896.725 kb on + strand, within AMB_RS17930 at 3896.726 kb on - strand, within AMB_RS17930 at 3896.726 kb on - strand, within AMB_RS17930 at 3896.899 kb on + strand, within AMB_RS17930 at 3896.899 kb on + strand, within AMB_RS17930 at 3896.899 kb on + strand, within AMB_RS17930 at 3896.900 kb on - strand, within AMB_RS17930 at 3896.900 kb on - strand, within AMB_RS17930 at 3897.034 kb on + strand at 3897.034 kb on + strand at 3897.138 kb on + strand at 3897.139 kb on - strand at 3897.139 kb on - strand at 3897.139 kb on - strand at 3897.165 kb on + strand, within AMB_RS17935 at 3897.165 kb on + strand, within AMB_RS17935 at 3897.166 kb on - strand, within AMB_RS17935 at 3897.166 kb on - strand, within AMB_RS17935 at 3897.166 kb on - strand, within AMB_RS17935 at 3897.336 kb on + strand, within AMB_RS17935 at 3897.336 kb on + strand, within AMB_RS17935 at 3897.336 kb on + strand, within AMB_RS17935 at 3897.336 kb on + strand, within AMB_RS17935 at 3897.336 kb on + strand, within AMB_RS17935 at 3897.337 kb on - strand, within AMB_RS17935 at 3897.337 kb on - strand, within AMB_RS17935 at 3897.337 kb on - strand, within AMB_RS17935 at 3897.474 kb on + strand, within AMB_RS17935 at 3897.474 kb on + strand, within AMB_RS17935 at 3897.474 kb on + strand, within AMB_RS17935 at 3897.474 kb on + strand, within AMB_RS17935 at 3897.474 kb on + strand, within AMB_RS17935 at 3897.475 kb on - strand, within AMB_RS17935 at 3897.475 kb on - strand, within AMB_RS17935 at 3897.475 kb on - strand, within AMB_RS17935 at 3897.475 kb on - strand, within AMB_RS17935 at 3897.475 kb on - strand, within AMB_RS17935 at 3897.528 kb on + strand, within AMB_RS17935 at 3897.529 kb on - strand, within AMB_RS17935 at 3897.573 kb on + strand, within AMB_RS17935 at 3897.574 kb on - strand, within AMB_RS17935 at 3897.574 kb on - strand, within AMB_RS17935 at 3897.972 kb on + strand at 3897.972 kb on + strand at 3897.972 kb on + strand at 3897.973 kb on - strand at 3897.975 kb on + strand at 3897.976 kb on - strand at 3897.976 kb on - strand at 3898.066 kb on - strand, within AMB_RS17940 at 3898.066 kb on - strand, within AMB_RS17940 at 3898.066 kb on - strand, within AMB_RS17940 at 3898.066 kb on - strand, within AMB_RS17940 at 3898.066 kb on - strand, within AMB_RS17940 at 3898.165 kb on + strand, within AMB_RS17940 at 3898.217 kb on + strand, within AMB_RS17940 at 3898.217 kb on + strand, within AMB_RS17940 at 3898.217 kb on + strand, within AMB_RS17940 at 3898.217 kb on + strand, within AMB_RS17940 at 3898.218 kb on - strand, within AMB_RS17940 at 3898.218 kb on - strand, within AMB_RS17940 at 3898.218 kb on - strand, within AMB_RS17940 at 3898.219 kb on + strand at 3898.219 kb on + strand at 3898.220 kb on - strand at 3898.220 kb on - strand at 3898.220 kb on - strand at 3898.220 kb on - strand at 3898.220 kb on - strand at 3898.220 kb on - strand at 3898.220 kb on - strand at 3898.238 kb on + strand at 3898.238 kb on + strand at 3898.238 kb on + strand at 3898.239 kb on - strand at 3898.284 kb on + strand, within AMB_RS17945 at 3898.284 kb on + strand, within AMB_RS17945 at 3898.284 kb on + strand, within AMB_RS17945 at 3898.284 kb on + strand, within AMB_RS17945 at 3898.285 kb on - strand, within AMB_RS17945 at 3898.378 kb on - strand, within AMB_RS17945 at 3898.444 kb on + strand at 3898.444 kb on + strand at 3898.611 kb on - strand at 3898.626 kb on + strand at 3898.631 kb on + strand at 3898.631 kb on + strand at 3898.631 kb on + strand at 3898.631 kb on + strand at 3898.632 kb on - strand at 3898.639 kb on - strand at 3898.644 kb on - strand at 3898.644 kb on - strand at 3898.705 kb on - strand at 3898.727 kb on - strand at 3898.766 kb on + strand at 3898.766 kb on + strand at 3898.766 kb on + strand at 3898.767 kb on - strand at 3898.767 kb on - strand at 3898.767 kb on - strand at 3898.767 kb on - strand at 3898.767 kb on - strand at 3898.854 kb on - strand, within AMB_RS17950
Per-strain Table
Position Strand Gene LocusTag Fraction 150_mag_3 remove 3,896,153 + -0.8 3,896,470 + AMB_RS17930 0.23 -1.1 3,896,470 + AMB_RS17930 0.23 -1.3 3,896,470 + AMB_RS17930 0.23 -0.8 3,896,470 + AMB_RS17930 0.23 -0.7 3,896,471 - AMB_RS17930 0.23 -1.3 3,896,471 - AMB_RS17930 0.23 -1.6 3,896,471 - AMB_RS17930 0.23 -1.6 3,896,575 + AMB_RS17930 0.37 -1.0 3,896,575 + AMB_RS17930 0.37 -0.7 3,896,575 + AMB_RS17930 0.37 +0.2 3,896,576 - AMB_RS17930 0.38 -1.0 3,896,576 - AMB_RS17930 0.38 -0.8 3,896,576 - AMB_RS17930 0.38 -1.0 3,896,576 - AMB_RS17930 0.38 -2.5 3,896,576 - AMB_RS17930 0.38 -0.7 3,896,576 - AMB_RS17930 0.38 -0.6 3,896,576 - AMB_RS17930 0.38 -0.5 3,896,611 + AMB_RS17930 0.42 -1.5 3,896,612 - AMB_RS17930 0.42 -1.2 3,896,635 + AMB_RS17930 0.46 -1.4 3,896,635 + AMB_RS17930 0.46 -3.1 3,896,636 - AMB_RS17930 0.46 -1.6 3,896,636 - AMB_RS17930 0.46 +0.1 3,896,636 - AMB_RS17930 0.46 -1.3 3,896,636 - AMB_RS17930 0.46 -0.8 3,896,725 + AMB_RS17930 0.58 -1.2 3,896,725 + AMB_RS17930 0.58 -1.6 3,896,725 + AMB_RS17930 0.58 -1.3 3,896,726 - AMB_RS17930 0.58 -1.9 3,896,726 - AMB_RS17930 0.58 -1.8 3,896,899 + AMB_RS17930 0.81 +1.1 3,896,899 + AMB_RS17930 0.81 -0.6 3,896,899 + AMB_RS17930 0.81 -1.4 3,896,900 - AMB_RS17930 0.81 -1.5 3,896,900 - AMB_RS17930 0.81 +0.3 3,897,034 + -0.8 3,897,034 + -0.8 3,897,138 + -0.7 3,897,139 - -1.3 3,897,139 - -2.2 3,897,139 - -1.1 3,897,165 + AMB_RS17935 0.12 -2.0 3,897,165 + AMB_RS17935 0.12 -0.7 3,897,166 - AMB_RS17935 0.12 -1.8 3,897,166 - AMB_RS17935 0.12 -0.7 3,897,166 - AMB_RS17935 0.12 -1.8 3,897,336 + AMB_RS17935 0.30 -1.7 3,897,336 + AMB_RS17935 0.30 -1.1 3,897,336 + AMB_RS17935 0.30 -1.2 3,897,336 + AMB_RS17935 0.30 -1.5 3,897,336 + AMB_RS17935 0.30 -1.3 3,897,337 - AMB_RS17935 0.30 -1.5 3,897,337 - AMB_RS17935 0.30 -2.0 3,897,337 - AMB_RS17935 0.30 -1.2 3,897,474 + AMB_RS17935 0.45 -1.9 3,897,474 + AMB_RS17935 0.45 -2.3 3,897,474 + AMB_RS17935 0.45 -3.2 3,897,474 + AMB_RS17935 0.45 -1.1 3,897,474 + AMB_RS17935 0.45 -0.7 3,897,475 - AMB_RS17935 0.45 -0.9 3,897,475 - AMB_RS17935 0.45 +0.7 3,897,475 - AMB_RS17935 0.45 -1.4 3,897,475 - AMB_RS17935 0.45 -0.8 3,897,475 - AMB_RS17935 0.45 -1.0 3,897,528 + AMB_RS17935 0.51 -0.7 3,897,529 - AMB_RS17935 0.51 -0.2 3,897,573 + AMB_RS17935 0.55 -0.3 3,897,574 - AMB_RS17935 0.56 -0.9 3,897,574 - AMB_RS17935 0.56 -0.6 3,897,972 + -0.6 3,897,972 + +0.2 3,897,972 + -0.9 3,897,973 - -1.9 3,897,975 + +0.0 3,897,976 - -1.3 3,897,976 - -1.4 3,898,066 - AMB_RS17940 0.14 -0.5 3,898,066 - AMB_RS17940 0.14 +1.1 3,898,066 - AMB_RS17940 0.14 -0.1 3,898,066 - AMB_RS17940 0.14 -0.5 3,898,066 - AMB_RS17940 0.14 -1.0 3,898,165 + AMB_RS17940 0.63 -0.6 3,898,217 + AMB_RS17940 0.89 -1.0 3,898,217 + AMB_RS17940 0.89 -0.2 3,898,217 + AMB_RS17940 0.89 -0.8 3,898,217 + AMB_RS17940 0.89 -1.0 3,898,218 - AMB_RS17940 0.90 -0.4 3,898,218 - AMB_RS17940 0.90 -0.4 3,898,218 - AMB_RS17940 0.90 -0.9 3,898,219 + -0.1 3,898,219 + -0.2 3,898,220 - +0.0 3,898,220 - -1.3 3,898,220 - -0.2 3,898,220 - +0.1 3,898,220 - -0.3 3,898,220 - -0.7 3,898,220 - -0.2 3,898,238 + -1.4 3,898,238 + +0.3 3,898,238 + -0.4 3,898,239 - +1.6 3,898,284 + AMB_RS17945 0.16 -0.3 3,898,284 + AMB_RS17945 0.16 -1.6 3,898,284 + AMB_RS17945 0.16 +0.1 3,898,284 + AMB_RS17945 0.16 -1.3 3,898,285 - AMB_RS17945 0.17 +0.1 3,898,378 - AMB_RS17945 0.65 -0.1 3,898,444 + -0.2 3,898,444 + -0.6 3,898,611 - +0.3 3,898,626 + -0.8 3,898,631 + -0.9 3,898,631 + +2.4 3,898,631 + +1.1 3,898,631 + -0.0 3,898,632 - +0.1 3,898,639 - +0.3 3,898,644 - -0.4 3,898,644 - -1.1 3,898,705 - -0.5 3,898,727 - +0.1 3,898,766 + -1.9 3,898,766 + -1.2 3,898,766 + -0.1 3,898,767 - +0.8 3,898,767 - -0.1 3,898,767 - -0.4 3,898,767 - -0.8 3,898,767 - -0.8 3,898,854 - AMB_RS17950 0.24 +0.3
Or see this region's nucleotide sequence