Experiment: 150_mag_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS12260 and AMB_RS12265 are separated by 276 nucleotides AMB_RS12265 and AMB_RS12270 overlap by 1 nucleotides AMB_RS12270 and AMB_RS12275 overlap by 17 nucleotides AMB_RS12275 and AMB_RS12280 overlap by 1 nucleotides AMB_RS12280 and AMB_RS12285 are separated by 442 nucleotides
AMB_RS12260: AMB_RS12260 - Rep protein, at 2,618,220 to 2,619,482
_RS12260
AMB_RS12265: AMB_RS12265 - hypothetical protein, at 2,619,759 to 2,619,971
_RS12265
AMB_RS12270: AMB_RS12270 - hypothetical protein, at 2,619,971 to 2,620,276
_RS12270
AMB_RS12275: AMB_RS12275 - hypothetical protein, at 2,620,260 to 2,620,463
_RS12275
AMB_RS12280: AMB_RS12280 - DUF736 domain-containing protein, at 2,620,463 to 2,620,828
_RS12280
AMB_RS12285: AMB_RS12285 - Na+/H+ antiporter NhaA, at 2,621,271 to 2,622,422
_RS12285
Position (kb)
2620
2621 Strain fitness (log2 ratio)
-1
0
1 at 2621.201 kb on + strand at 2621.201 kb on + strand at 2621.202 kb on - strand at 2621.267 kb on + strand at 2621.268 kb on - strand at 2621.268 kb on - strand at 2621.272 kb on + strand at 2621.272 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 150_mag_3 remove 2,621,201 + -0.3 2,621,201 + -0.1 2,621,202 - -0.9 2,621,267 + -0.3 2,621,268 - -0.2 2,621,268 - +0.7 2,621,272 + +1.3 2,621,272 + -0.3
Or see this region's nucleotide sequence