Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS20445 and ABIE40_RS20450 overlap by 4 nucleotides ABIE40_RS20450 and ABIE40_RS20455 overlap by 4 nucleotides ABIE40_RS20455 and ABIE40_RS20460 are separated by 2 nucleotides
ABIE40_RS20445: ABIE40_RS20445 - precorrin-3B C(17)-methyltransferase, at 193,168 to 193,932
_RS20445
ABIE40_RS20450: ABIE40_RS20450 - precorrin-2 C(20)-methyltransferase, at 193,929 to 194,660
_RS20450
ABIE40_RS20455: ABIE40_RS20455 - precorrin-8X methylmutase, at 194,657 to 195,289
_RS20455
ABIE40_RS20460: ABIE40_RS20460 - precorrin-3B synthase, at 195,292 to 196,656
_RS20460
Position (kb) 194
195
196 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 193.909 kb on + strand at 193.910 kb on - strand at 193.916 kb on + strand at 193.916 kb on + strand at 193.917 kb on - strand at 193.917 kb on - strand at 193.917 kb on - strand at 193.917 kb on - strand at 193.973 kb on - strand at 193.973 kb on - strand at 194.312 kb on - strand, within ABIE40_RS20450 at 194.312 kb on - strand, within ABIE40_RS20450 at 194.312 kb on - strand, within ABIE40_RS20450 at 194.320 kb on + strand, within ABIE40_RS20450 at 194.357 kb on + strand, within ABIE40_RS20450 at 194.358 kb on - strand, within ABIE40_RS20450 at 194.405 kb on - strand, within ABIE40_RS20450 at 194.661 kb on - strand at 194.661 kb on - strand at 194.661 kb on - strand at 194.661 kb on - strand at 194.860 kb on - strand, within ABIE40_RS20455 at 194.948 kb on - strand, within ABIE40_RS20455 at 194.948 kb on - strand, within ABIE40_RS20455 at 195.249 kb on + strand at 195.250 kb on - strand at 195.273 kb on + strand at 195.273 kb on + strand at 195.274 kb on - strand at 195.279 kb on + strand at 195.293 kb on + strand at 195.294 kb on - strand at 195.623 kb on + strand, within ABIE40_RS20460 at 195.667 kb on - strand, within ABIE40_RS20460 at 196.112 kb on - strand, within ABIE40_RS20460 at 196.112 kb on - strand, within ABIE40_RS20460 at 196.143 kb on + strand, within ABIE40_RS20460
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 193,909 + -3.2 193,910 - -1.3 193,916 + -0.8 193,916 + -2.1 193,917 - -0.8 193,917 - -0.5 193,917 - +1.1 193,917 - -0.9 193,973 - -2.6 193,973 - -3.0 194,312 - ABIE40_RS20450 0.52 -3.6 194,312 - ABIE40_RS20450 0.52 -2.0 194,312 - ABIE40_RS20450 0.52 -0.3 194,320 + ABIE40_RS20450 0.53 -1.9 194,357 + ABIE40_RS20450 0.58 -0.6 194,358 - ABIE40_RS20450 0.59 -1.8 194,405 - ABIE40_RS20450 0.65 -1.6 194,661 - -0.4 194,661 - -1.2 194,661 - -0.7 194,661 - -0.9 194,860 - ABIE40_RS20455 0.32 -1.2 194,948 - ABIE40_RS20455 0.46 -3.0 194,948 - ABIE40_RS20455 0.46 -0.6 195,249 + -1.3 195,250 - -2.3 195,273 + -0.1 195,273 + -0.6 195,274 - -1.2 195,279 + -0.3 195,293 + -2.2 195,294 - -0.9 195,623 + ABIE40_RS20460 0.24 -2.8 195,667 - ABIE40_RS20460 0.27 -2.5 196,112 - ABIE40_RS20460 0.60 -1.2 196,112 - ABIE40_RS20460 0.60 -2.2 196,143 + ABIE40_RS20460 0.62 -1.8
Or see this region's nucleotide sequence