Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS18920 and ABIE40_RS18925 overlap by 8 nucleotides ABIE40_RS18925 and ABIE40_RS18930 are separated by 37 nucleotides ABIE40_RS18930 and ABIE40_RS18935 are separated by 265 nucleotides
ABIE40_RS18920: ABIE40_RS18920 - glutamate 5-kinase, at 3,790,996 to 3,792,165
_RS18920
ABIE40_RS18925: ABIE40_RS18925 - glutamate-5-semialdehyde dehydrogenase, at 3,792,158 to 3,793,441
_RS18925
ABIE40_RS18930: ABIE40_RS18930 - nicotinate-nucleotide adenylyltransferase, at 3,793,479 to 3,794,063
_RS18930
ABIE40_RS18935: ABIE40_RS18935 - class I SAM-dependent methyltransferase, at 3,794,329 to 3,795,114
_RS18935
Position (kb)
3792
3793
3794 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 3791.538 kb on + strand, within ABIE40_RS18920 at 3791.850 kb on + strand, within ABIE40_RS18920 at 3791.943 kb on + strand, within ABIE40_RS18920 at 3791.943 kb on + strand, within ABIE40_RS18920 at 3791.943 kb on + strand, within ABIE40_RS18920 at 3791.998 kb on + strand, within ABIE40_RS18920 at 3791.998 kb on + strand, within ABIE40_RS18920 at 3792.157 kb on + strand at 3792.157 kb on + strand at 3792.157 kb on + strand at 3792.157 kb on + strand at 3792.166 kb on + strand at 3792.166 kb on + strand at 3792.166 kb on + strand at 3792.166 kb on + strand at 3792.166 kb on + strand at 3792.877 kb on + strand, within ABIE40_RS18925 at 3792.877 kb on + strand, within ABIE40_RS18925 at 3792.877 kb on + strand, within ABIE40_RS18925 at 3792.881 kb on + strand, within ABIE40_RS18925 at 3792.881 kb on + strand, within ABIE40_RS18925 at 3792.881 kb on + strand, within ABIE40_RS18925 at 3792.881 kb on + strand, within ABIE40_RS18925 at 3792.881 kb on + strand, within ABIE40_RS18925 at 3792.881 kb on + strand, within ABIE40_RS18925 at 3792.881 kb on + strand, within ABIE40_RS18925 at 3792.881 kb on + strand, within ABIE40_RS18925 at 3792.881 kb on + strand, within ABIE40_RS18925 at 3793.124 kb on + strand, within ABIE40_RS18925 at 3793.162 kb on + strand, within ABIE40_RS18925 at 3793.193 kb on + strand, within ABIE40_RS18925 at 3793.406 kb on + strand at 3794.061 kb on + strand at 3794.124 kb on + strand at 3794.124 kb on + strand at 3794.125 kb on - strand at 3794.206 kb on + strand at 3794.206 kb on + strand at 3794.207 kb on - strand at 3794.213 kb on + strand at 3794.218 kb on + strand at 3794.278 kb on - strand at 3794.282 kb on + strand at 3794.287 kb on - strand at 3794.384 kb on + strand at 3794.384 kb on + strand at 3794.384 kb on + strand at 3794.385 kb on - strand at 3794.385 kb on - strand at 3794.385 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 3,791,538 + ABIE40_RS18920 0.46 -6.0 3,791,850 + ABIE40_RS18920 0.73 -4.3 3,791,943 + ABIE40_RS18920 0.81 -3.2 3,791,943 + ABIE40_RS18920 0.81 -1.1 3,791,943 + ABIE40_RS18920 0.81 -2.0 3,791,998 + ABIE40_RS18920 0.86 -4.9 3,791,998 + ABIE40_RS18920 0.86 -3.3 3,792,157 + -2.8 3,792,157 + -2.3 3,792,157 + +1.7 3,792,157 + -3.3 3,792,166 + -2.8 3,792,166 + -0.8 3,792,166 + -3.4 3,792,166 + -4.3 3,792,166 + -2.5 3,792,877 + ABIE40_RS18925 0.56 +0.2 3,792,877 + ABIE40_RS18925 0.56 +1.7 3,792,877 + ABIE40_RS18925 0.56 -0.6 3,792,881 + ABIE40_RS18925 0.56 -4.7 3,792,881 + ABIE40_RS18925 0.56 -1.3 3,792,881 + ABIE40_RS18925 0.56 -2.1 3,792,881 + ABIE40_RS18925 0.56 -1.7 3,792,881 + ABIE40_RS18925 0.56 -2.0 3,792,881 + ABIE40_RS18925 0.56 -1.8 3,792,881 + ABIE40_RS18925 0.56 -2.1 3,792,881 + ABIE40_RS18925 0.56 -3.6 3,792,881 + ABIE40_RS18925 0.56 -2.1 3,793,124 + ABIE40_RS18925 0.75 -4.2 3,793,162 + ABIE40_RS18925 0.78 -3.7 3,793,193 + ABIE40_RS18925 0.81 -3.0 3,793,406 + -3.8 3,794,061 + +0.1 3,794,124 + -0.2 3,794,124 + -1.0 3,794,125 - -0.0 3,794,206 + +0.5 3,794,206 + -0.6 3,794,207 - -0.3 3,794,213 + -0.4 3,794,218 + -0.2 3,794,278 - +1.2 3,794,282 + +0.9 3,794,287 - +0.3 3,794,384 + -3.0 3,794,384 + +0.1 3,794,384 + +1.7 3,794,385 - -0.3 3,794,385 - -0.4 3,794,385 - -2.0
Or see this region's nucleotide sequence