Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS09930 and ABIE40_RS09935 are separated by 76 nucleotides ABIE40_RS09935 and ABIE40_RS09940 are separated by 402 nucleotides ABIE40_RS09940 and ABIE40_RS09945 are separated by 24 nucleotides ABIE40_RS09945 and ABIE40_RS09950 overlap by 1 nucleotides
ABIE40_RS09930: ABIE40_RS09930 - copper chaperone PCu(A)C, at 1,911,059 to 1,912,045
_RS09930
ABIE40_RS09935: ABIE40_RS09935 - hypothetical protein, at 1,912,122 to 1,912,502
_RS09935
ABIE40_RS09940: ABIE40_RS09940 - glycine cleavage system aminomethyltransferase GcvT, at 1,912,905 to 1,914,041
_RS09940
ABIE40_RS09945: ABIE40_RS09945 - glycine cleavage system protein GcvH, at 1,914,066 to 1,914,428
_RS09945
ABIE40_RS09950: ABIE40_RS09950 - aminomethyl-transferring glycine dehydrogenase, at 1,914,428 to 1,917,292
_RS09950
Position (kb)
1912
1913
1914
1915 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1912.187 kb on + strand, within ABIE40_RS09935 at 1912.187 kb on + strand, within ABIE40_RS09935 at 1912.188 kb on - strand, within ABIE40_RS09935 at 1912.188 kb on - strand, within ABIE40_RS09935 at 1912.188 kb on - strand, within ABIE40_RS09935 at 1912.188 kb on - strand, within ABIE40_RS09935 at 1912.188 kb on - strand, within ABIE40_RS09935 at 1912.373 kb on + strand, within ABIE40_RS09935 at 1912.373 kb on + strand, within ABIE40_RS09935 at 1912.373 kb on + strand, within ABIE40_RS09935 at 1912.373 kb on + strand, within ABIE40_RS09935 at 1912.373 kb on + strand, within ABIE40_RS09935 at 1912.374 kb on - strand, within ABIE40_RS09935 at 1912.374 kb on - strand, within ABIE40_RS09935 at 1912.375 kb on + strand, within ABIE40_RS09935 at 1912.375 kb on + strand, within ABIE40_RS09935 at 1912.375 kb on + strand, within ABIE40_RS09935 at 1912.376 kb on - strand, within ABIE40_RS09935 at 1912.376 kb on - strand, within ABIE40_RS09935 at 1912.389 kb on - strand, within ABIE40_RS09935 at 1912.391 kb on - strand, within ABIE40_RS09935 at 1912.531 kb on - strand at 1912.539 kb on + strand at 1912.540 kb on - strand at 1912.800 kb on - strand at 1912.913 kb on + strand at 1912.989 kb on + strand at 1912.989 kb on + strand at 1913.007 kb on + strand at 1913.076 kb on - strand, within ABIE40_RS09940 at 1913.112 kb on + strand, within ABIE40_RS09940 at 1913.112 kb on + strand, within ABIE40_RS09940 at 1913.112 kb on + strand, within ABIE40_RS09940 at 1913.112 kb on + strand, within ABIE40_RS09940 at 1913.112 kb on + strand, within ABIE40_RS09940 at 1913.112 kb on + strand, within ABIE40_RS09940 at 1913.113 kb on - strand, within ABIE40_RS09940 at 1913.113 kb on - strand, within ABIE40_RS09940 at 1913.113 kb on - strand, within ABIE40_RS09940 at 1913.113 kb on - strand, within ABIE40_RS09940 at 1913.113 kb on - strand, within ABIE40_RS09940 at 1913.113 kb on - strand, within ABIE40_RS09940 at 1913.113 kb on - strand, within ABIE40_RS09940 at 1913.216 kb on + strand, within ABIE40_RS09940 at 1913.303 kb on + strand, within ABIE40_RS09940 at 1913.303 kb on + strand, within ABIE40_RS09940 at 1913.303 kb on + strand, within ABIE40_RS09940 at 1913.303 kb on + strand, within ABIE40_RS09940 at 1913.487 kb on + strand, within ABIE40_RS09940 at 1913.631 kb on + strand, within ABIE40_RS09940 at 1913.632 kb on - strand, within ABIE40_RS09940 at 1913.838 kb on + strand, within ABIE40_RS09940 at 1913.838 kb on + strand, within ABIE40_RS09940 at 1913.838 kb on + strand, within ABIE40_RS09940 at 1913.839 kb on - strand, within ABIE40_RS09940 at 1913.839 kb on - strand, within ABIE40_RS09940 at 1913.839 kb on - strand, within ABIE40_RS09940 at 1913.839 kb on - strand, within ABIE40_RS09940 at 1913.839 kb on - strand, within ABIE40_RS09940 at 1913.925 kb on + strand, within ABIE40_RS09940 at 1913.926 kb on - strand, within ABIE40_RS09940 at 1913.926 kb on - strand, within ABIE40_RS09940 at 1913.926 kb on - strand, within ABIE40_RS09940 at 1913.926 kb on - strand, within ABIE40_RS09940 at 1913.986 kb on - strand at 1913.986 kb on - strand at 1914.018 kb on - strand at 1914.018 kb on - strand at 1914.020 kb on + strand at 1914.020 kb on + strand at 1914.021 kb on - strand at 1914.030 kb on + strand at 1914.031 kb on - strand at 1914.078 kb on - strand at 1914.128 kb on + strand, within ABIE40_RS09945 at 1914.128 kb on + strand, within ABIE40_RS09945 at 1914.128 kb on + strand, within ABIE40_RS09945 at 1914.128 kb on + strand, within ABIE40_RS09945 at 1914.131 kb on + strand, within ABIE40_RS09945 at 1914.132 kb on - strand, within ABIE40_RS09945 at 1914.132 kb on - strand, within ABIE40_RS09945 at 1914.132 kb on - strand, within ABIE40_RS09945 at 1914.138 kb on + strand, within ABIE40_RS09945 at 1914.139 kb on - strand, within ABIE40_RS09945 at 1914.166 kb on + strand, within ABIE40_RS09945 at 1914.167 kb on - strand, within ABIE40_RS09945 at 1914.167 kb on - strand, within ABIE40_RS09945 at 1914.255 kb on + strand, within ABIE40_RS09945 at 1914.255 kb on + strand, within ABIE40_RS09945 at 1914.255 kb on + strand, within ABIE40_RS09945 at 1914.255 kb on + strand, within ABIE40_RS09945 at 1914.255 kb on + strand, within ABIE40_RS09945 at 1914.255 kb on + strand, within ABIE40_RS09945 at 1914.256 kb on - strand, within ABIE40_RS09945 at 1914.256 kb on - strand, within ABIE40_RS09945 at 1914.408 kb on + strand at 1914.409 kb on - strand at 1914.426 kb on + strand at 1914.461 kb on + strand at 1914.461 kb on + strand at 1914.493 kb on + strand at 1914.523 kb on + strand at 1914.523 kb on + strand at 1914.523 kb on + strand at 1914.542 kb on + strand at 1914.542 kb on + strand at 1914.542 kb on + strand at 1914.543 kb on - strand at 1914.544 kb on + strand at 1914.544 kb on + strand at 1914.544 kb on + strand at 1914.544 kb on + strand at 1914.544 kb on + strand at 1914.545 kb on - strand at 1914.545 kb on - strand at 1914.708 kb on - strand at 1914.708 kb on - strand at 1914.773 kb on + strand, within ABIE40_RS09950 at 1914.773 kb on + strand, within ABIE40_RS09950 at 1914.774 kb on - strand, within ABIE40_RS09950 at 1914.774 kb on - strand, within ABIE40_RS09950 at 1914.775 kb on + strand, within ABIE40_RS09950 at 1914.775 kb on + strand, within ABIE40_RS09950 at 1914.776 kb on - strand, within ABIE40_RS09950 at 1914.827 kb on + strand, within ABIE40_RS09950 at 1914.828 kb on - strand, within ABIE40_RS09950 at 1914.828 kb on - strand, within ABIE40_RS09950 at 1914.987 kb on + strand, within ABIE40_RS09950 at 1914.988 kb on - strand, within ABIE40_RS09950 at 1914.988 kb on - strand, within ABIE40_RS09950 at 1914.988 kb on - strand, within ABIE40_RS09950 at 1914.988 kb on - strand, within ABIE40_RS09950
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 1,912,187 + ABIE40_RS09935 0.17 -0.4 1,912,187 + ABIE40_RS09935 0.17 -2.0 1,912,188 - ABIE40_RS09935 0.17 +2.1 1,912,188 - ABIE40_RS09935 0.17 +1.7 1,912,188 - ABIE40_RS09935 0.17 -1.8 1,912,188 - ABIE40_RS09935 0.17 -0.4 1,912,188 - ABIE40_RS09935 0.17 -0.2 1,912,373 + ABIE40_RS09935 0.66 -1.6 1,912,373 + ABIE40_RS09935 0.66 -0.6 1,912,373 + ABIE40_RS09935 0.66 +0.1 1,912,373 + ABIE40_RS09935 0.66 +2.2 1,912,373 + ABIE40_RS09935 0.66 -0.6 1,912,374 - ABIE40_RS09935 0.66 -1.4 1,912,374 - ABIE40_RS09935 0.66 -1.5 1,912,375 + ABIE40_RS09935 0.66 +2.1 1,912,375 + ABIE40_RS09935 0.66 -0.8 1,912,375 + ABIE40_RS09935 0.66 +1.2 1,912,376 - ABIE40_RS09935 0.67 -0.5 1,912,376 - ABIE40_RS09935 0.67 +1.2 1,912,389 - ABIE40_RS09935 0.70 +0.2 1,912,391 - ABIE40_RS09935 0.71 -1.8 1,912,531 - -0.0 1,912,539 + +0.2 1,912,540 - +0.2 1,912,800 - -0.1 1,912,913 + -2.0 1,912,989 + +0.4 1,912,989 + -0.3 1,913,007 + -2.2 1,913,076 - ABIE40_RS09940 0.15 -0.6 1,913,112 + ABIE40_RS09940 0.18 -1.9 1,913,112 + ABIE40_RS09940 0.18 -2.3 1,913,112 + ABIE40_RS09940 0.18 -1.8 1,913,112 + ABIE40_RS09940 0.18 -0.3 1,913,112 + ABIE40_RS09940 0.18 -2.2 1,913,112 + ABIE40_RS09940 0.18 -2.4 1,913,113 - ABIE40_RS09940 0.18 +0.4 1,913,113 - ABIE40_RS09940 0.18 -1.4 1,913,113 - ABIE40_RS09940 0.18 -1.5 1,913,113 - ABIE40_RS09940 0.18 -1.0 1,913,113 - ABIE40_RS09940 0.18 -1.7 1,913,113 - ABIE40_RS09940 0.18 -0.8 1,913,113 - ABIE40_RS09940 0.18 +3.2 1,913,216 + ABIE40_RS09940 0.27 -0.2 1,913,303 + ABIE40_RS09940 0.35 -2.7 1,913,303 + ABIE40_RS09940 0.35 -0.4 1,913,303 + ABIE40_RS09940 0.35 +0.9 1,913,303 + ABIE40_RS09940 0.35 -2.2 1,913,487 + ABIE40_RS09940 0.51 -2.8 1,913,631 + ABIE40_RS09940 0.64 -2.6 1,913,632 - ABIE40_RS09940 0.64 -1.1 1,913,838 + ABIE40_RS09940 0.82 -0.1 1,913,838 + ABIE40_RS09940 0.82 -2.0 1,913,838 + ABIE40_RS09940 0.82 -1.6 1,913,839 - ABIE40_RS09940 0.82 +0.7 1,913,839 - ABIE40_RS09940 0.82 -2.0 1,913,839 - ABIE40_RS09940 0.82 -0.0 1,913,839 - ABIE40_RS09940 0.82 -3.8 1,913,839 - ABIE40_RS09940 0.82 +0.9 1,913,925 + ABIE40_RS09940 0.90 -1.0 1,913,926 - ABIE40_RS09940 0.90 -1.1 1,913,926 - ABIE40_RS09940 0.90 -1.2 1,913,926 - ABIE40_RS09940 0.90 -2.3 1,913,926 - ABIE40_RS09940 0.90 -2.2 1,913,986 - -1.6 1,913,986 - -2.6 1,914,018 - -3.2 1,914,018 - +1.2 1,914,020 + +0.7 1,914,020 + +0.6 1,914,021 - -0.3 1,914,030 + -0.8 1,914,031 - -1.1 1,914,078 - -1.7 1,914,128 + ABIE40_RS09945 0.17 -1.4 1,914,128 + ABIE40_RS09945 0.17 -0.4 1,914,128 + ABIE40_RS09945 0.17 -2.8 1,914,128 + ABIE40_RS09945 0.17 -1.6 1,914,131 + ABIE40_RS09945 0.18 -2.2 1,914,132 - ABIE40_RS09945 0.18 -0.6 1,914,132 - ABIE40_RS09945 0.18 -1.1 1,914,132 - ABIE40_RS09945 0.18 -3.2 1,914,138 + ABIE40_RS09945 0.20 +1.6 1,914,139 - ABIE40_RS09945 0.20 -3.2 1,914,166 + ABIE40_RS09945 0.28 -1.6 1,914,167 - ABIE40_RS09945 0.28 -2.0 1,914,167 - ABIE40_RS09945 0.28 -0.7 1,914,255 + ABIE40_RS09945 0.52 -1.0 1,914,255 + ABIE40_RS09945 0.52 -1.6 1,914,255 + ABIE40_RS09945 0.52 -0.8 1,914,255 + ABIE40_RS09945 0.52 -1.8 1,914,255 + ABIE40_RS09945 0.52 -1.6 1,914,255 + ABIE40_RS09945 0.52 -1.0 1,914,256 - ABIE40_RS09945 0.52 -1.9 1,914,256 - ABIE40_RS09945 0.52 -1.7 1,914,408 + -1.2 1,914,409 - -2.0 1,914,426 + -1.0 1,914,461 + -2.0 1,914,461 + -3.2 1,914,493 + +0.3 1,914,523 + -1.4 1,914,523 + -2.0 1,914,523 + -2.4 1,914,542 + -2.1 1,914,542 + -2.3 1,914,542 + -0.6 1,914,543 - -3.3 1,914,544 + +1.2 1,914,544 + -0.8 1,914,544 + -2.3 1,914,544 + -2.1 1,914,544 + -2.6 1,914,545 - -3.1 1,914,545 - -1.2 1,914,708 - -1.9 1,914,708 - -3.1 1,914,773 + ABIE40_RS09950 0.12 -1.0 1,914,773 + ABIE40_RS09950 0.12 +0.6 1,914,774 - ABIE40_RS09950 0.12 -3.1 1,914,774 - ABIE40_RS09950 0.12 -1.0 1,914,775 + ABIE40_RS09950 0.12 -1.8 1,914,775 + ABIE40_RS09950 0.12 -3.5 1,914,776 - ABIE40_RS09950 0.12 +0.2 1,914,827 + ABIE40_RS09950 0.14 -2.2 1,914,828 - ABIE40_RS09950 0.14 +0.0 1,914,828 - ABIE40_RS09950 0.14 -2.4 1,914,987 + ABIE40_RS09950 0.20 -2.2 1,914,988 - ABIE40_RS09950 0.20 -1.3 1,914,988 - ABIE40_RS09950 0.20 -1.4 1,914,988 - ABIE40_RS09950 0.20 -1.7 1,914,988 - ABIE40_RS09950 0.20 -0.5
Or see this region's nucleotide sequence