Experiment: 150_mag_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS21290 and AMB_RS21295 are separated by 6 nucleotides AMB_RS21295 and AMB_RS21300 are separated by 38 nucleotides AMB_RS21300 and AMB_RS21305 are separated by 412 nucleotides
AMB_RS21290: AMB_RS21290 - DUF4139 domain-containing protein, at 4,620,927 to 4,622,288
_RS21290
AMB_RS21295: AMB_RS21295 - hypothetical protein, at 4,622,295 to 4,622,537
_RS21295
AMB_RS21300: AMB_RS21300 - hypothetical protein, at 4,622,576 to 4,622,782
_RS21300
AMB_RS21305: AMB_RS21305 - DNA polymerase I, at 4,623,195 to 4,625,990
_RS21305
Position (kb) 4622
4623 Strain fitness (log2 ratio) -2
-1
0
1 at 4621.686 kb on + strand, within AMB_RS21290 at 4621.695 kb on + strand, within AMB_RS21290 at 4621.695 kb on + strand, within AMB_RS21290 at 4621.696 kb on - strand, within AMB_RS21290 at 4622.286 kb on + strand at 4622.287 kb on - strand at 4622.461 kb on + strand, within AMB_RS21295 at 4622.474 kb on + strand, within AMB_RS21295 at 4622.474 kb on + strand, within AMB_RS21295 at 4622.746 kb on - strand, within AMB_RS21300 at 4622.919 kb on + strand at 4623.101 kb on - strand at 4623.106 kb on + strand at 4623.107 kb on - strand at 4623.139 kb on - strand at 4623.139 kb on - strand at 4623.181 kb on + strand at 4623.182 kb on - strand at 4623.182 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 150_mag_2 remove 4,621,686 + AMB_RS21290 0.56 -2.3 4,621,695 + AMB_RS21290 0.56 -2.3 4,621,695 + AMB_RS21290 0.56 -2.5 4,621,696 - AMB_RS21290 0.56 -1.9 4,622,286 + -0.5 4,622,287 - -1.1 4,622,461 + AMB_RS21295 0.68 -0.2 4,622,474 + AMB_RS21295 0.74 +0.2 4,622,474 + AMB_RS21295 0.74 -0.1 4,622,746 - AMB_RS21300 0.82 -0.2 4,622,919 + -1.3 4,623,101 - -0.5 4,623,106 + +0.1 4,623,107 - +1.1 4,623,139 - -0.1 4,623,139 - -0.9 4,623,181 + +0.3 4,623,182 - -0.2 4,623,182 - -0.9
Or see this region's nucleotide sequence