Experiment: Fumarate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABID97_RS12580 and ABID97_RS12585 are separated by 1 nucleotides ABID97_RS12585 and ABID97_RS12590 overlap by 1 nucleotides ABID97_RS12590 and ABID97_RS12595 are separated by 83 nucleotides ABID97_RS12595 and ABID97_RS12600 are separated by 94 nucleotides
ABID97_RS12580: ABID97_RS12580 - methenyltetrahydromethanopterin cyclohydrolase, at 2,612,163 to 2,613,161
_RS12580
ABID97_RS12585: ABID97_RS12585 - RimK family alpha-L-glutamate ligase, at 2,613,163 to 2,614,110
_RS12585
ABID97_RS12590: ABID97_RS12590 - triphosphoribosyl-dephospho-CoA synthase, at 2,614,110 to 2,615,048
_RS12590
ABID97_RS12595: ABID97_RS12595 - formaldehyde-activating enzyme, at 2,615,132 to 2,615,644
_RS12595
ABID97_RS12600: ABID97_RS12600 - dihydroneopterin aldolase, at 2,615,739 to 2,616,170
_RS12600
Position (kb) 2614
2615
2616 Strain fitness (log2 ratio) -1
0
1 at 2614.758 kb on + strand, within ABID97_RS12590 at 2614.758 kb on + strand, within ABID97_RS12590 at 2615.049 kb on + strand at 2615.088 kb on - strand at 2615.088 kb on - strand at 2615.197 kb on + strand, within ABID97_RS12595
Per-strain Table
Position Strand Gene LocusTag Fraction Fumarate (C) remove 2,614,758 + ABID97_RS12590 0.69 -0.1 2,614,758 + ABID97_RS12590 0.69 +0.1 2,615,049 + -0.0 2,615,088 - +0.8 2,615,088 - +0.1 2,615,197 + ABID97_RS12595 0.13 +1.0
Or see this region's nucleotide sequence