Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_4446 and EX28DRAFT_4447 are separated by 93 nucleotides EX28DRAFT_4447 and EX28DRAFT_4448 are separated by 50 nucleotides EX28DRAFT_4448 and EX28DRAFT_4449 are separated by 112 nucleotides
EX28DRAFT_4446: EX28DRAFT_4446 - ketol-acid reductoisomerase (EC 1.1.1.86), at 11,311 to 12,786
_4446
EX28DRAFT_4447: EX28DRAFT_4447 - PPIC-type PPIASE domain, at 12,880 to 13,161
_4447
EX28DRAFT_4448: EX28DRAFT_4448 - PPIC-type PPIASE domain, at 13,212 to 13,493
_4448
EX28DRAFT_4449: EX28DRAFT_4449 - ATP-dependent DNA helicase Rep (EC 3.6.1.-), at 13,606 to 15,630
_4449
Position (kb) 13
14 Strain fitness (log2 ratio) -2
-1
0
1 at 12.343 kb on + strand, within EX28DRAFT_4446 at 12.387 kb on - strand, within EX28DRAFT_4446 at 12.387 kb on - strand, within EX28DRAFT_4446 at 12.473 kb on - strand, within EX28DRAFT_4446 at 12.570 kb on + strand, within EX28DRAFT_4446 at 12.574 kb on + strand, within EX28DRAFT_4446 at 12.575 kb on - strand, within EX28DRAFT_4446 at 12.615 kb on + strand, within EX28DRAFT_4446 at 12.616 kb on - strand, within EX28DRAFT_4446 at 12.616 kb on - strand, within EX28DRAFT_4446 at 12.848 kb on + strand at 12.881 kb on + strand at 12.881 kb on + strand at 12.882 kb on - strand at 12.882 kb on - strand at 12.896 kb on - strand at 12.904 kb on - strand at 12.904 kb on - strand at 13.044 kb on + strand, within EX28DRAFT_4447 at 13.044 kb on + strand, within EX28DRAFT_4447 at 13.045 kb on - strand, within EX28DRAFT_4447 at 13.105 kb on + strand, within EX28DRAFT_4447 at 13.105 kb on + strand, within EX28DRAFT_4447 at 13.106 kb on - strand, within EX28DRAFT_4447 at 13.176 kb on + strand at 13.176 kb on + strand at 13.177 kb on - strand at 13.201 kb on + strand at 13.202 kb on - strand at 13.203 kb on + strand at 13.203 kb on + strand at 13.203 kb on + strand at 13.204 kb on - strand at 13.233 kb on - strand at 13.238 kb on + strand at 13.239 kb on - strand at 13.293 kb on - strand, within EX28DRAFT_4448 at 13.377 kb on - strand, within EX28DRAFT_4448 at 13.466 kb on + strand at 13.467 kb on - strand at 13.467 kb on - strand at 13.531 kb on + strand at 13.531 kb on + strand at 13.532 kb on - strand at 13.568 kb on - strand at 13.613 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 12,343 + EX28DRAFT_4446 0.70 -1.9 12,387 - EX28DRAFT_4446 0.73 -0.1 12,387 - EX28DRAFT_4446 0.73 -1.3 12,473 - EX28DRAFT_4446 0.79 +0.4 12,570 + EX28DRAFT_4446 0.85 -0.0 12,574 + EX28DRAFT_4446 0.86 -0.6 12,575 - EX28DRAFT_4446 0.86 +0.7 12,615 + EX28DRAFT_4446 0.88 -1.0 12,616 - EX28DRAFT_4446 0.88 +0.5 12,616 - EX28DRAFT_4446 0.88 +0.5 12,848 + -0.3 12,881 + +0.9 12,881 + +0.2 12,882 - +0.0 12,882 - +0.2 12,896 - -0.5 12,904 - +0.2 12,904 - +0.4 13,044 + EX28DRAFT_4447 0.58 +0.4 13,044 + EX28DRAFT_4447 0.58 +0.1 13,045 - EX28DRAFT_4447 0.59 +0.1 13,105 + EX28DRAFT_4447 0.80 +0.9 13,105 + EX28DRAFT_4447 0.80 +1.2 13,106 - EX28DRAFT_4447 0.80 +0.1 13,176 + -0.1 13,176 + +1.5 13,177 - +0.3 13,201 + -2.7 13,202 - -0.2 13,203 + +1.7 13,203 + +0.8 13,203 + +0.2 13,204 - -0.1 13,233 - -0.2 13,238 + +0.3 13,239 - -0.2 13,293 - EX28DRAFT_4448 0.29 -0.4 13,377 - EX28DRAFT_4448 0.59 -1.5 13,466 + -0.1 13,467 - +0.7 13,467 - +0.6 13,531 + -0.3 13,531 + +0.2 13,532 - +0.0 13,568 - -0.2 13,613 + -1.3
Or see this region's nucleotide sequence