Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_4358 and EX28DRAFT_4359 are separated by 14 nucleotides EX28DRAFT_4359 and EX28DRAFT_4360 are separated by 17 nucleotides EX28DRAFT_4360 and EX28DRAFT_4361 are separated by 155 nucleotides
EX28DRAFT_4358: EX28DRAFT_4358 - N-acyl-D-glucosamine 2-epimerase, at 23,586 to 24,827
_4358
EX28DRAFT_4359: EX28DRAFT_4359 - Tagatose-1,6-bisphosphate aldolase, at 24,842 to 25,717
_4359
EX28DRAFT_4360: EX28DRAFT_4360 - 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases, at 25,735 to 26,625
_4360
EX28DRAFT_4361: EX28DRAFT_4361 - Sugar kinases, ribokinase family, at 26,781 to 27,677
_4361
Position (kb) 25
26
27 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 25.232 kb on + strand, within EX28DRAFT_4359 at 25.233 kb on - strand, within EX28DRAFT_4359 at 25.233 kb on - strand, within EX28DRAFT_4359 at 25.246 kb on + strand, within EX28DRAFT_4359 at 25.506 kb on + strand, within EX28DRAFT_4359 at 25.694 kb on - strand at 25.707 kb on + strand at 25.707 kb on + strand at 25.707 kb on + strand at 25.707 kb on + strand at 25.708 kb on - strand at 26.099 kb on + strand, within EX28DRAFT_4360 at 26.100 kb on - strand, within EX28DRAFT_4360 at 26.100 kb on - strand, within EX28DRAFT_4360 at 26.103 kb on + strand, within EX28DRAFT_4360 at 26.110 kb on - strand, within EX28DRAFT_4360 at 26.347 kb on - strand, within EX28DRAFT_4360 at 26.347 kb on - strand, within EX28DRAFT_4360 at 26.727 kb on + strand at 26.745 kb on - strand at 26.862 kb on + strand at 26.862 kb on + strand at 26.863 kb on - strand at 27.030 kb on - strand, within EX28DRAFT_4361 at 27.109 kb on - strand, within EX28DRAFT_4361 at 27.154 kb on + strand, within EX28DRAFT_4361 at 27.181 kb on + strand, within EX28DRAFT_4361 at 27.408 kb on + strand, within EX28DRAFT_4361 at 27.408 kb on + strand, within EX28DRAFT_4361 at 27.482 kb on - strand, within EX28DRAFT_4361
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 25,232 + EX28DRAFT_4359 0.45 +0.6 25,233 - EX28DRAFT_4359 0.45 +0.0 25,233 - EX28DRAFT_4359 0.45 -2.4 25,246 + EX28DRAFT_4359 0.46 -0.3 25,506 + EX28DRAFT_4359 0.76 -0.9 25,694 - +0.5 25,707 + +0.3 25,707 + -0.6 25,707 + +0.6 25,707 + -0.6 25,708 - -0.2 26,099 + EX28DRAFT_4360 0.41 -1.5 26,100 - EX28DRAFT_4360 0.41 +0.2 26,100 - EX28DRAFT_4360 0.41 -3.5 26,103 + EX28DRAFT_4360 0.41 +0.1 26,110 - EX28DRAFT_4360 0.42 -0.0 26,347 - EX28DRAFT_4360 0.69 -0.1 26,347 - EX28DRAFT_4360 0.69 +0.1 26,727 + +0.1 26,745 - +0.0 26,862 + +0.2 26,862 + +0.0 26,863 - -0.4 27,030 - EX28DRAFT_4361 0.28 +1.1 27,109 - EX28DRAFT_4361 0.37 -0.2 27,154 + EX28DRAFT_4361 0.42 -0.1 27,181 + EX28DRAFT_4361 0.45 +1.6 27,408 + EX28DRAFT_4361 0.70 +0.0 27,408 + EX28DRAFT_4361 0.70 +0.5 27,482 - EX28DRAFT_4361 0.78 +0.1
Or see this region's nucleotide sequence