Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3602 and EX28DRAFT_3603 are separated by 55 nucleotides EX28DRAFT_3603 and EX28DRAFT_3604 are separated by 27 nucleotides EX28DRAFT_3604 and EX28DRAFT_3605 are separated by 50 nucleotides EX28DRAFT_3605 and EX28DRAFT_3606 overlap by 4 nucleotides
EX28DRAFT_3602: EX28DRAFT_3602 - Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains), at 261,273 to 263,210
_3602
EX28DRAFT_3603: EX28DRAFT_3603 - Trp operon repressor, at 263,266 to 263,595
_3603
EX28DRAFT_3604: EX28DRAFT_3604 - inosine/xanthosine triphosphatase, at 263,623 to 264,138
_3604
EX28DRAFT_3605: EX28DRAFT_3605 - Fructose-2,6-bisphosphatase, at 264,189 to 264,836
_3605
EX28DRAFT_3606: EX28DRAFT_3606 - AraC-type DNA-binding domain-containing proteins, at 264,833 to 265,702
_3606
Position (kb) 264
265 Strain fitness (log2 ratio) -2
-1
0
1 at 263.227 kb on + strand at 263.262 kb on + strand at 263.263 kb on - strand at 263.263 kb on - strand at 263.284 kb on + strand at 263.577 kb on - strand at 263.577 kb on - strand at 263.617 kb on - strand at 263.678 kb on + strand, within EX28DRAFT_3604 at 263.733 kb on + strand, within EX28DRAFT_3604 at 263.759 kb on + strand, within EX28DRAFT_3604 at 263.760 kb on - strand, within EX28DRAFT_3604 at 264.111 kb on + strand at 264.138 kb on + strand at 264.138 kb on + strand at 264.138 kb on + strand at 264.138 kb on + strand at 264.139 kb on - strand at 264.143 kb on - strand at 264.172 kb on + strand at 264.173 kb on - strand at 264.173 kb on - strand at 264.173 kb on - strand at 264.174 kb on + strand at 264.174 kb on + strand at 264.201 kb on + strand at 264.201 kb on + strand at 264.202 kb on - strand at 264.202 kb on - strand at 264.202 kb on - strand at 264.465 kb on - strand, within EX28DRAFT_3605 at 264.465 kb on - strand, within EX28DRAFT_3605 at 264.563 kb on + strand, within EX28DRAFT_3605 at 265.072 kb on + strand, within EX28DRAFT_3606 at 265.072 kb on + strand, within EX28DRAFT_3606 at 265.073 kb on - strand, within EX28DRAFT_3606 at 265.075 kb on - strand, within EX28DRAFT_3606 at 265.111 kb on - strand, within EX28DRAFT_3606 at 265.269 kb on + strand, within EX28DRAFT_3606 at 265.269 kb on + strand, within EX28DRAFT_3606 at 265.270 kb on - strand, within EX28DRAFT_3606 at 265.270 kb on - strand, within EX28DRAFT_3606 at 265.270 kb on - strand, within EX28DRAFT_3606 at 265.270 kb on - strand, within EX28DRAFT_3606 at 265.320 kb on + strand, within EX28DRAFT_3606 at 265.527 kb on - strand, within EX28DRAFT_3606 at 265.543 kb on + strand, within EX28DRAFT_3606 at 265.544 kb on - strand, within EX28DRAFT_3606 at 265.545 kb on + strand, within EX28DRAFT_3606 at 265.546 kb on - strand, within EX28DRAFT_3606 at 265.546 kb on - strand, within EX28DRAFT_3606 at 265.546 kb on - strand, within EX28DRAFT_3606 at 265.702 kb on + strand at 265.702 kb on + strand at 265.702 kb on + strand at 265.702 kb on + strand at 265.703 kb on - strand at 265.703 kb on - strand at 265.703 kb on - strand at 265.703 kb on - strand at 265.703 kb on - strand at 265.703 kb on - strand at 265.703 kb on - strand at 265.704 kb on + strand at 265.704 kb on + strand at 265.704 kb on + strand at 265.704 kb on + strand at 265.704 kb on + strand at 265.704 kb on + strand at 265.704 kb on + strand at 265.704 kb on + strand at 265.704 kb on + strand at 265.705 kb on - strand at 265.705 kb on - strand at 265.705 kb on - strand at 265.705 kb on - strand at 265.705 kb on - strand at 265.705 kb on - strand at 265.705 kb on - strand at 265.719 kb on + strand at 265.719 kb on + strand at 265.719 kb on + strand at 265.756 kb on + strand at 265.831 kb on - strand at 265.832 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 263,227 + +0.1 263,262 + +0.1 263,263 - +0.2 263,263 - -1.1 263,284 + +0.2 263,577 - +0.2 263,577 - -0.9 263,617 - -0.6 263,678 + EX28DRAFT_3604 0.11 -0.5 263,733 + EX28DRAFT_3604 0.21 -0.0 263,759 + EX28DRAFT_3604 0.26 -2.2 263,760 - EX28DRAFT_3604 0.27 +1.4 264,111 + +0.6 264,138 + -1.2 264,138 + -0.9 264,138 + -0.8 264,138 + +0.9 264,139 - -1.4 264,143 - -1.5 264,172 + -0.5 264,173 - +1.0 264,173 - -0.1 264,173 - -0.4 264,174 + -0.3 264,174 + -0.6 264,201 + -0.7 264,201 + +1.3 264,202 - -0.8 264,202 - -1.2 264,202 - -0.4 264,465 - EX28DRAFT_3605 0.43 -0.1 264,465 - EX28DRAFT_3605 0.43 -2.0 264,563 + EX28DRAFT_3605 0.58 -1.8 265,072 + EX28DRAFT_3606 0.27 +0.3 265,072 + EX28DRAFT_3606 0.27 -0.8 265,073 - EX28DRAFT_3606 0.28 +0.2 265,075 - EX28DRAFT_3606 0.28 +0.2 265,111 - EX28DRAFT_3606 0.32 +0.2 265,269 + EX28DRAFT_3606 0.50 -0.5 265,269 + EX28DRAFT_3606 0.50 +0.6 265,270 - EX28DRAFT_3606 0.50 -1.2 265,270 - EX28DRAFT_3606 0.50 +0.8 265,270 - EX28DRAFT_3606 0.50 +0.5 265,270 - EX28DRAFT_3606 0.50 +1.2 265,320 + EX28DRAFT_3606 0.56 -0.1 265,527 - EX28DRAFT_3606 0.80 +0.1 265,543 + EX28DRAFT_3606 0.82 +0.1 265,544 - EX28DRAFT_3606 0.82 -0.7 265,545 + EX28DRAFT_3606 0.82 -0.1 265,546 - EX28DRAFT_3606 0.82 -0.5 265,546 - EX28DRAFT_3606 0.82 -0.7 265,546 - EX28DRAFT_3606 0.82 -0.4 265,702 + -1.6 265,702 + -0.7 265,702 + +0.4 265,702 + +0.3 265,703 - -0.5 265,703 - -0.2 265,703 - -0.4 265,703 - -0.2 265,703 - +0.1 265,703 - +0.1 265,703 - -0.0 265,704 + -0.7 265,704 + -0.6 265,704 + -0.2 265,704 + +0.4 265,704 + -0.3 265,704 + +0.7 265,704 + -0.4 265,704 + +0.9 265,704 + +0.1 265,705 - -0.3 265,705 - +0.3 265,705 - +1.0 265,705 - -0.2 265,705 - +0.2 265,705 - -0.1 265,705 - +0.3 265,719 + +0.5 265,719 + -0.5 265,719 + -1.0 265,756 + +0.9 265,831 - -0.8 265,832 + -0.8
Or see this region's nucleotide sequence