Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3559 and EX28DRAFT_3560 are separated by 270 nucleotides EX28DRAFT_3560 and EX28DRAFT_3561 are separated by 80 nucleotides EX28DRAFT_3561 and EX28DRAFT_3562 overlap by 4 nucleotides
EX28DRAFT_3559: EX28DRAFT_3559 - Phosphoglycerol transferase and related proteins, alkaline phosphatase superfamily, at 222,522 to 224,813
_3559
EX28DRAFT_3560: EX28DRAFT_3560 - Protein of unknown function (DUF2501), at 225,084 to 225,569
_3560
EX28DRAFT_3561: EX28DRAFT_3561 - DNA replication protein, at 225,650 to 226,387
_3561
EX28DRAFT_3562: EX28DRAFT_3562 - hypothetical protein, at 226,384 to 226,929
_3562
Position (kb)
225
226 Strain fitness (log2 ratio)
-1
0
1 at 224.084 kb on + strand, within EX28DRAFT_3559 at 224.084 kb on + strand, within EX28DRAFT_3559 at 224.085 kb on - strand, within EX28DRAFT_3559 at 224.183 kb on + strand, within EX28DRAFT_3559 at 224.184 kb on - strand, within EX28DRAFT_3559 at 224.311 kb on + strand, within EX28DRAFT_3559 at 224.312 kb on - strand, within EX28DRAFT_3559 at 224.317 kb on + strand, within EX28DRAFT_3559 at 224.318 kb on - strand, within EX28DRAFT_3559 at 224.365 kb on + strand, within EX28DRAFT_3559 at 224.365 kb on + strand, within EX28DRAFT_3559 at 224.365 kb on + strand, within EX28DRAFT_3559 at 224.365 kb on + strand, within EX28DRAFT_3559 at 224.365 kb on + strand, within EX28DRAFT_3559 at 224.368 kb on + strand, within EX28DRAFT_3559 at 224.368 kb on + strand, within EX28DRAFT_3559 at 224.369 kb on - strand, within EX28DRAFT_3559 at 224.484 kb on - strand, within EX28DRAFT_3559 at 224.587 kb on + strand at 224.588 kb on - strand at 224.588 kb on - strand at 224.588 kb on - strand at 224.588 kb on - strand at 224.649 kb on - strand at 224.666 kb on - strand at 224.685 kb on - strand at 224.819 kb on + strand at 224.819 kb on + strand at 224.871 kb on + strand at 224.872 kb on - strand at 224.872 kb on - strand at 224.872 kb on - strand at 224.878 kb on + strand at 224.879 kb on - strand at 224.879 kb on - strand at 225.227 kb on + strand, within EX28DRAFT_3560 at 225.228 kb on - strand, within EX28DRAFT_3560 at 225.228 kb on - strand, within EX28DRAFT_3560 at 225.229 kb on + strand, within EX28DRAFT_3560 at 225.230 kb on - strand, within EX28DRAFT_3560 at 225.230 kb on - strand, within EX28DRAFT_3560 at 225.230 kb on - strand, within EX28DRAFT_3560 at 225.230 kb on - strand, within EX28DRAFT_3560 at 225.230 kb on - strand, within EX28DRAFT_3560 at 225.230 kb on - strand, within EX28DRAFT_3560 at 225.392 kb on + strand, within EX28DRAFT_3560 at 225.393 kb on - strand, within EX28DRAFT_3560 at 225.393 kb on - strand, within EX28DRAFT_3560 at 225.393 kb on - strand, within EX28DRAFT_3560 at 225.549 kb on + strand at 225.613 kb on - strand at 225.613 kb on - strand at 225.613 kb on - strand at 225.614 kb on + strand at 225.614 kb on + strand at 225.614 kb on + strand at 225.615 kb on - strand at 225.615 kb on - strand at 225.615 kb on - strand at 225.616 kb on + strand at 225.616 kb on + strand at 225.616 kb on + strand at 225.616 kb on + strand at 225.616 kb on + strand at 225.616 kb on + strand at 225.616 kb on + strand at 225.617 kb on - strand at 225.617 kb on - strand at 225.617 kb on - strand at 225.617 kb on - strand at 225.617 kb on - strand at 225.617 kb on - strand at 225.617 kb on - strand at 225.617 kb on - strand at 225.617 kb on - strand at 225.617 kb on - strand at 225.617 kb on - strand at 225.617 kb on - strand at 225.617 kb on - strand at 225.617 kb on - strand at 225.617 kb on - strand at 225.620 kb on + strand at 225.621 kb on - strand at 225.621 kb on - strand at 225.621 kb on - strand at 225.621 kb on - strand at 225.652 kb on - strand at 225.652 kb on - strand at 225.655 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 224,084 + EX28DRAFT_3559 0.68 +0.6 224,084 + EX28DRAFT_3559 0.68 +0.1 224,085 - EX28DRAFT_3559 0.68 +0.1 224,183 + EX28DRAFT_3559 0.72 +0.0 224,184 - EX28DRAFT_3559 0.73 +0.5 224,311 + EX28DRAFT_3559 0.78 -0.1 224,312 - EX28DRAFT_3559 0.78 -0.9 224,317 + EX28DRAFT_3559 0.78 +1.2 224,318 - EX28DRAFT_3559 0.78 -0.5 224,365 + EX28DRAFT_3559 0.80 -1.0 224,365 + EX28DRAFT_3559 0.80 +0.2 224,365 + EX28DRAFT_3559 0.80 -0.7 224,365 + EX28DRAFT_3559 0.80 +1.1 224,365 + EX28DRAFT_3559 0.80 -1.2 224,368 + EX28DRAFT_3559 0.81 -0.4 224,368 + EX28DRAFT_3559 0.81 -0.1 224,369 - EX28DRAFT_3559 0.81 -0.0 224,484 - EX28DRAFT_3559 0.86 -0.2 224,587 + +0.6 224,588 - -0.1 224,588 - -0.1 224,588 - +0.5 224,588 - -0.4 224,649 - +0.2 224,666 - +0.2 224,685 - -0.3 224,819 + -0.3 224,819 + +0.3 224,871 + +0.0 224,872 - -0.1 224,872 - -0.5 224,872 - +0.1 224,878 + -0.0 224,879 - +0.3 224,879 - +0.1 225,227 + EX28DRAFT_3560 0.29 -1.0 225,228 - EX28DRAFT_3560 0.30 +0.0 225,228 - EX28DRAFT_3560 0.30 -0.3 225,229 + EX28DRAFT_3560 0.30 -0.3 225,230 - EX28DRAFT_3560 0.30 -0.3 225,230 - EX28DRAFT_3560 0.30 -0.5 225,230 - EX28DRAFT_3560 0.30 -0.8 225,230 - EX28DRAFT_3560 0.30 +0.4 225,230 - EX28DRAFT_3560 0.30 +0.5 225,230 - EX28DRAFT_3560 0.30 -0.7 225,392 + EX28DRAFT_3560 0.63 -1.0 225,393 - EX28DRAFT_3560 0.64 -0.6 225,393 - EX28DRAFT_3560 0.64 -0.2 225,393 - EX28DRAFT_3560 0.64 -1.6 225,549 + -0.1 225,613 - -0.8 225,613 - -0.6 225,613 - -0.6 225,614 + +0.3 225,614 + +0.4 225,614 + -1.4 225,615 - -0.6 225,615 - -0.3 225,615 - -0.7 225,616 + +0.3 225,616 + +0.0 225,616 + +0.6 225,616 + -0.2 225,616 + -0.1 225,616 + -0.6 225,616 + +0.3 225,617 - +0.5 225,617 - +0.0 225,617 - +0.2 225,617 - +0.8 225,617 - -0.4 225,617 - +0.8 225,617 - -0.7 225,617 - -0.3 225,617 - +0.4 225,617 - +0.4 225,617 - +0.5 225,617 - -0.9 225,617 - -0.7 225,617 - -1.3 225,617 - -0.0 225,620 + -0.6 225,621 - +0.4 225,621 - -1.6 225,621 - +0.0 225,621 - -0.3 225,652 - -0.1 225,652 - +0.7 225,655 - +0.3
Or see this region's nucleotide sequence