Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3530 and EX28DRAFT_3531 are separated by 44 nucleotides EX28DRAFT_3531 and EX28DRAFT_3532 overlap by 1 nucleotides EX28DRAFT_3532 and EX28DRAFT_3533 are separated by 411 nucleotides
EX28DRAFT_3530: EX28DRAFT_3530 - NAD-dependent aldehyde dehydrogenases, at 192,822 to 194,192
_3530
EX28DRAFT_3531: EX28DRAFT_3531 - hypothetical protein, at 194,237 to 194,557
_3531
EX28DRAFT_3532: EX28DRAFT_3532 - YfaZ precursor, at 194,557 to 195,102
_3532
EX28DRAFT_3533: EX28DRAFT_3533 - Uncharacterized protein conserved in bacteria, at 195,514 to 196,872
_3533
Position (kb) 194
195
196 Strain fitness (log2 ratio) -2
-1
0
1 at 193.629 kb on - strand, within EX28DRAFT_3530 at 193.809 kb on + strand, within EX28DRAFT_3530 at 193.809 kb on + strand, within EX28DRAFT_3530 at 193.869 kb on - strand, within EX28DRAFT_3530 at 193.869 kb on - strand, within EX28DRAFT_3530 at 193.869 kb on - strand, within EX28DRAFT_3530 at 193.891 kb on - strand, within EX28DRAFT_3530 at 193.927 kb on - strand, within EX28DRAFT_3530 at 193.977 kb on + strand, within EX28DRAFT_3530 at 194.058 kb on + strand at 194.058 kb on + strand at 194.059 kb on - strand at 194.059 kb on - strand at 194.059 kb on - strand at 194.186 kb on + strand at 194.187 kb on - strand at 194.190 kb on + strand at 194.289 kb on - strand, within EX28DRAFT_3531 at 194.293 kb on + strand, within EX28DRAFT_3531 at 194.293 kb on + strand, within EX28DRAFT_3531 at 194.293 kb on + strand, within EX28DRAFT_3531 at 194.294 kb on - strand, within EX28DRAFT_3531 at 194.329 kb on - strand, within EX28DRAFT_3531 at 194.341 kb on + strand, within EX28DRAFT_3531 at 194.579 kb on - strand at 194.582 kb on + strand at 194.582 kb on + strand at 194.670 kb on + strand, within EX28DRAFT_3532 at 194.670 kb on + strand, within EX28DRAFT_3532 at 194.709 kb on - strand, within EX28DRAFT_3532 at 194.747 kb on + strand, within EX28DRAFT_3532 at 194.748 kb on - strand, within EX28DRAFT_3532 at 194.748 kb on - strand, within EX28DRAFT_3532 at 194.964 kb on - strand, within EX28DRAFT_3532 at 195.107 kb on + strand at 195.296 kb on - strand at 195.417 kb on + strand at 195.419 kb on + strand at 195.419 kb on + strand at 195.419 kb on + strand at 195.419 kb on + strand at 195.420 kb on - strand at 195.420 kb on - strand at 195.497 kb on - strand at 195.497 kb on - strand at 195.507 kb on + strand at 195.513 kb on + strand at 195.514 kb on - strand at 195.631 kb on + strand at 195.699 kb on + strand, within EX28DRAFT_3533 at 195.794 kb on + strand, within EX28DRAFT_3533 at 195.889 kb on - strand, within EX28DRAFT_3533
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 193,629 - EX28DRAFT_3530 0.59 -0.4 193,809 + EX28DRAFT_3530 0.72 -0.1 193,809 + EX28DRAFT_3530 0.72 +0.8 193,869 - EX28DRAFT_3530 0.76 -0.7 193,869 - EX28DRAFT_3530 0.76 +0.2 193,869 - EX28DRAFT_3530 0.76 +0.1 193,891 - EX28DRAFT_3530 0.78 +0.2 193,927 - EX28DRAFT_3530 0.81 +0.1 193,977 + EX28DRAFT_3530 0.84 -2.6 194,058 + +0.3 194,058 + -1.0 194,059 - -0.2 194,059 - -1.2 194,059 - +0.0 194,186 + -0.8 194,187 - -2.6 194,190 + +1.6 194,289 - EX28DRAFT_3531 0.16 +1.7 194,293 + EX28DRAFT_3531 0.17 -0.2 194,293 + EX28DRAFT_3531 0.17 +0.2 194,293 + EX28DRAFT_3531 0.17 -0.5 194,294 - EX28DRAFT_3531 0.18 -0.3 194,329 - EX28DRAFT_3531 0.29 +0.2 194,341 + EX28DRAFT_3531 0.32 -0.5 194,579 - -0.2 194,582 + +0.0 194,582 + +0.6 194,670 + EX28DRAFT_3532 0.21 -0.4 194,670 + EX28DRAFT_3532 0.21 -1.6 194,709 - EX28DRAFT_3532 0.28 -0.4 194,747 + EX28DRAFT_3532 0.35 -1.3 194,748 - EX28DRAFT_3532 0.35 -0.4 194,748 - EX28DRAFT_3532 0.35 -1.7 194,964 - EX28DRAFT_3532 0.75 +0.1 195,107 + +0.7 195,296 - -0.4 195,417 + -0.1 195,419 + -0.6 195,419 + -0.8 195,419 + -0.2 195,419 + -1.0 195,420 - +0.7 195,420 - -0.1 195,497 - +0.5 195,497 - -1.2 195,507 + +0.3 195,513 + -0.3 195,514 - -0.6 195,631 + -0.8 195,699 + EX28DRAFT_3533 0.14 +0.8 195,794 + EX28DRAFT_3533 0.21 -0.4 195,889 - EX28DRAFT_3533 0.28 -0.3
Or see this region's nucleotide sequence