Strain Fitness in Enterobacter asburiae PDN3 around EX28DRAFT_3032

Experiment: M9 with casaminos and trichloroethylene 76.3 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntEX28DRAFT_3030 and EX28DRAFT_3031 overlap by 11 nucleotidesEX28DRAFT_3031 and EX28DRAFT_3032 are separated by 24 nucleotidesEX28DRAFT_3032 and EX28DRAFT_3033 are separated by 141 nucleotidesEX28DRAFT_3033 and EX28DRAFT_3034 are separated by 10 nucleotides EX28DRAFT_3030: EX28DRAFT_3030 - Esterase/lipase, at 341,461 to 342,363 _3030 EX28DRAFT_3031: EX28DRAFT_3031 - Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily), at 342,353 to 343,249 _3031 EX28DRAFT_3032: EX28DRAFT_3032 - Sugar phosphate isomerases/epimerases, at 343,274 to 344,329 _3032 EX28DRAFT_3033: EX28DRAFT_3033 - transcriptional regulator, TetR family, at 344,471 to 345,061 _3033 EX28DRAFT_3034: EX28DRAFT_3034 - hypothetical protein, at 345,072 to 345,350 _3034 Position (kb) 343 344 345Strain fitness (log2 ratio) -3 -2 -1 0 1at 342.352 kb on - strandat 342.389 kb on + strandat 342.430 kb on + strandat 342.431 kb on - strandat 342.485 kb on + strand, within EX28DRAFT_3031at 342.653 kb on - strand, within EX28DRAFT_3031at 342.653 kb on - strand, within EX28DRAFT_3031at 342.654 kb on + strand, within EX28DRAFT_3031at 342.654 kb on + strand, within EX28DRAFT_3031at 342.654 kb on + strand, within EX28DRAFT_3031at 342.654 kb on + strand, within EX28DRAFT_3031at 342.655 kb on - strand, within EX28DRAFT_3031at 342.655 kb on - strand, within EX28DRAFT_3031at 342.655 kb on - strand, within EX28DRAFT_3031at 342.655 kb on - strand, within EX28DRAFT_3031at 342.655 kb on - strand, within EX28DRAFT_3031at 342.655 kb on - strand, within EX28DRAFT_3031at 342.655 kb on - strand, within EX28DRAFT_3031at 342.655 kb on - strand, within EX28DRAFT_3031at 342.704 kb on - strand, within EX28DRAFT_3031at 342.704 kb on - strand, within EX28DRAFT_3031at 342.715 kb on - strand, within EX28DRAFT_3031at 342.715 kb on - strand, within EX28DRAFT_3031at 342.929 kb on + strand, within EX28DRAFT_3031at 342.932 kb on + strand, within EX28DRAFT_3031at 342.932 kb on + strand, within EX28DRAFT_3031at 342.932 kb on + strand, within EX28DRAFT_3031at 342.932 kb on + strand, within EX28DRAFT_3031at 342.933 kb on - strand, within EX28DRAFT_3031at 342.937 kb on + strand, within EX28DRAFT_3031at 342.937 kb on + strand, within EX28DRAFT_3031at 342.938 kb on - strand, within EX28DRAFT_3031at 342.938 kb on - strand, within EX28DRAFT_3031at 342.938 kb on - strand, within EX28DRAFT_3031at 342.938 kb on - strand, within EX28DRAFT_3031at 342.938 kb on - strand, within EX28DRAFT_3031at 342.938 kb on - strand, within EX28DRAFT_3031at 342.938 kb on - strand, within EX28DRAFT_3031at 342.938 kb on - strand, within EX28DRAFT_3031at 342.971 kb on - strand, within EX28DRAFT_3031at 343.092 kb on + strand, within EX28DRAFT_3031at 343.092 kb on + strand, within EX28DRAFT_3031at 343.093 kb on - strand, within EX28DRAFT_3031at 343.102 kb on + strand, within EX28DRAFT_3031at 343.105 kb on - strand, within EX28DRAFT_3031at 343.194 kb on + strandat 343.194 kb on + strandat 343.195 kb on - strandat 343.236 kb on + strandat 343.289 kb on + strandat 343.361 kb on - strandat 343.452 kb on + strand, within EX28DRAFT_3032at 343.481 kb on - strand, within EX28DRAFT_3032at 343.557 kb on + strand, within EX28DRAFT_3032at 343.558 kb on - strand, within EX28DRAFT_3032at 343.561 kb on + strand, within EX28DRAFT_3032at 343.626 kb on + strand, within EX28DRAFT_3032at 343.648 kb on - strand, within EX28DRAFT_3032at 343.665 kb on - strand, within EX28DRAFT_3032at 343.723 kb on - strand, within EX28DRAFT_3032at 343.789 kb on + strand, within EX28DRAFT_3032at 344.043 kb on - strand, within EX28DRAFT_3032at 344.063 kb on + strand, within EX28DRAFT_3032at 344.063 kb on + strand, within EX28DRAFT_3032at 344.064 kb on - strand, within EX28DRAFT_3032at 344.131 kb on + strand, within EX28DRAFT_3032at 344.263 kb on + strandat 344.332 kb on + strandat 344.332 kb on + strandat 344.332 kb on + strandat 344.333 kb on - strandat 344.333 kb on - strandat 344.334 kb on + strandat 344.334 kb on + strandat 344.335 kb on - strandat 344.335 kb on - strandat 344.371 kb on - strandat 344.397 kb on + strandat 344.426 kb on - strandat 344.426 kb on - strandat 344.546 kb on + strand, within EX28DRAFT_3033at 344.547 kb on - strand, within EX28DRAFT_3033at 344.607 kb on - strand, within EX28DRAFT_3033at 344.785 kb on + strand, within EX28DRAFT_3033at 344.785 kb on + strand, within EX28DRAFT_3033at 344.785 kb on + strand, within EX28DRAFT_3033at 344.785 kb on + strand, within EX28DRAFT_3033at 344.785 kb on + strand, within EX28DRAFT_3033at 344.790 kb on + strand, within EX28DRAFT_3033at 344.951 kb on + strand, within EX28DRAFT_3033at 344.951 kb on + strand, within EX28DRAFT_3033at 345.175 kb on + strand, within EX28DRAFT_3034at 345.242 kb on + strand, within EX28DRAFT_3034at 345.243 kb on - strand, within EX28DRAFT_3034at 345.247 kb on - strand, within EX28DRAFT_3034at 345.247 kb on - strand, within EX28DRAFT_3034at 345.251 kb on + strand, within EX28DRAFT_3034at 345.251 kb on + strand, within EX28DRAFT_3034at 345.251 kb on + strand, within EX28DRAFT_3034at 345.252 kb on - strand, within EX28DRAFT_3034at 345.306 kb on - strand, within EX28DRAFT_3034

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM
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342,352 - -0.2
342,389 + +0.2
342,430 + -0.5
342,431 - -0.1
342,485 + EX28DRAFT_3031 0.15 -0.2
342,653 - EX28DRAFT_3031 0.33 -0.0
342,653 - EX28DRAFT_3031 0.33 +0.3
342,654 + EX28DRAFT_3031 0.34 -0.5
342,654 + EX28DRAFT_3031 0.34 +0.4
342,654 + EX28DRAFT_3031 0.34 -0.1
342,654 + EX28DRAFT_3031 0.34 +0.4
342,655 - EX28DRAFT_3031 0.34 -0.2
342,655 - EX28DRAFT_3031 0.34 -0.0
342,655 - EX28DRAFT_3031 0.34 +1.4
342,655 - EX28DRAFT_3031 0.34 -0.6
342,655 - EX28DRAFT_3031 0.34 -0.2
342,655 - EX28DRAFT_3031 0.34 +0.8
342,655 - EX28DRAFT_3031 0.34 +0.4
342,655 - EX28DRAFT_3031 0.34 -2.0
342,704 - EX28DRAFT_3031 0.39 +0.6
342,704 - EX28DRAFT_3031 0.39 -1.5
342,715 - EX28DRAFT_3031 0.40 +0.5
342,715 - EX28DRAFT_3031 0.40 +0.1
342,929 + EX28DRAFT_3031 0.64 +0.4
342,932 + EX28DRAFT_3031 0.65 +0.1
342,932 + EX28DRAFT_3031 0.65 -0.3
342,932 + EX28DRAFT_3031 0.65 -1.0
342,932 + EX28DRAFT_3031 0.65 -0.6
342,933 - EX28DRAFT_3031 0.65 -0.3
342,937 + EX28DRAFT_3031 0.65 -3.0
342,937 + EX28DRAFT_3031 0.65 -0.6
342,938 - EX28DRAFT_3031 0.65 +0.4
342,938 - EX28DRAFT_3031 0.65 +0.2
342,938 - EX28DRAFT_3031 0.65 +0.4
342,938 - EX28DRAFT_3031 0.65 +0.1
342,938 - EX28DRAFT_3031 0.65 +0.0
342,938 - EX28DRAFT_3031 0.65 +0.4
342,938 - EX28DRAFT_3031 0.65 -1.3
342,938 - EX28DRAFT_3031 0.65 -0.8
342,971 - EX28DRAFT_3031 0.69 -0.8
343,092 + EX28DRAFT_3031 0.82 +0.4
343,092 + EX28DRAFT_3031 0.82 +0.1
343,093 - EX28DRAFT_3031 0.82 -0.8
343,102 + EX28DRAFT_3031 0.84 +0.1
343,105 - EX28DRAFT_3031 0.84 +0.4
343,194 + +0.3
343,194 + +0.2
343,195 - -0.3
343,236 + +0.6
343,289 + -0.4
343,361 - -0.0
343,452 + EX28DRAFT_3032 0.17 +0.1
343,481 - EX28DRAFT_3032 0.20 -0.3
343,557 + EX28DRAFT_3032 0.27 -0.8
343,558 - EX28DRAFT_3032 0.27 +0.2
343,561 + EX28DRAFT_3032 0.27 -0.2
343,626 + EX28DRAFT_3032 0.33 -1.1
343,648 - EX28DRAFT_3032 0.35 -0.7
343,665 - EX28DRAFT_3032 0.37 +0.1
343,723 - EX28DRAFT_3032 0.43 -1.4
343,789 + EX28DRAFT_3032 0.49 -0.2
344,043 - EX28DRAFT_3032 0.73 +0.2
344,063 + EX28DRAFT_3032 0.75 -0.4
344,063 + EX28DRAFT_3032 0.75 -0.3
344,064 - EX28DRAFT_3032 0.75 -0.1
344,131 + EX28DRAFT_3032 0.81 -0.2
344,263 + +0.3
344,332 + +0.2
344,332 + -0.7
344,332 + +0.0
344,333 - +0.9
344,333 - -0.5
344,334 + +0.2
344,334 + +0.4
344,335 - -0.0
344,335 - -1.4
344,371 - -1.6
344,397 + +0.2
344,426 - +0.9
344,426 - -1.4
344,546 + EX28DRAFT_3033 0.13 +1.0
344,547 - EX28DRAFT_3033 0.13 -0.8
344,607 - EX28DRAFT_3033 0.23 -1.1
344,785 + EX28DRAFT_3033 0.53 -0.8
344,785 + EX28DRAFT_3033 0.53 -0.5
344,785 + EX28DRAFT_3033 0.53 -0.2
344,785 + EX28DRAFT_3033 0.53 -0.7
344,785 + EX28DRAFT_3033 0.53 +0.4
344,790 + EX28DRAFT_3033 0.54 -1.3
344,951 + EX28DRAFT_3033 0.81 +0.3
344,951 + EX28DRAFT_3033 0.81 -0.1
345,175 + EX28DRAFT_3034 0.37 +0.8
345,242 + EX28DRAFT_3034 0.61 -0.1
345,243 - EX28DRAFT_3034 0.61 +0.8
345,247 - EX28DRAFT_3034 0.63 +1.0
345,247 - EX28DRAFT_3034 0.63 +0.2
345,251 + EX28DRAFT_3034 0.64 -0.2
345,251 + EX28DRAFT_3034 0.64 +0.2
345,251 + EX28DRAFT_3034 0.64 -0.5
345,252 - EX28DRAFT_3034 0.65 +0.4
345,306 - EX28DRAFT_3034 0.84 +0.0

Or see this region's nucleotide sequence