Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2864 and EX28DRAFT_2865 are separated by 51 nucleotides EX28DRAFT_2865 and EX28DRAFT_2866 are separated by 57 nucleotides
EX28DRAFT_2864: EX28DRAFT_2864 - (p)ppGpp synthetase, RelA/SpoT family, at 166,835 to 169,066
_2864
EX28DRAFT_2865: EX28DRAFT_2865 - 23S rRNA (uracil-5-)-methyltransferase RumA, at 169,118 to 170,416
_2865
EX28DRAFT_2866: EX28DRAFT_2866 - Uncharacterized domain of BarA-like signal transduction histidine kinases, at 170,474 to 173,233
_2866
Position (kb)
169
170
171 Strain fitness (log2 ratio)
-1
0
1 at 168.139 kb on - strand, within EX28DRAFT_2864 at 168.182 kb on + strand, within EX28DRAFT_2864 at 168.183 kb on - strand, within EX28DRAFT_2864 at 168.298 kb on - strand, within EX28DRAFT_2864 at 168.302 kb on - strand, within EX28DRAFT_2864 at 169.080 kb on - strand at 169.120 kb on - strand at 169.260 kb on + strand, within EX28DRAFT_2865 at 169.260 kb on + strand, within EX28DRAFT_2865 at 169.260 kb on + strand, within EX28DRAFT_2865 at 169.260 kb on + strand, within EX28DRAFT_2865 at 169.261 kb on - strand, within EX28DRAFT_2865 at 169.285 kb on + strand, within EX28DRAFT_2865 at 169.288 kb on + strand, within EX28DRAFT_2865 at 169.289 kb on - strand, within EX28DRAFT_2865 at 169.289 kb on - strand, within EX28DRAFT_2865 at 169.291 kb on + strand, within EX28DRAFT_2865 at 169.291 kb on + strand, within EX28DRAFT_2865 at 169.291 kb on + strand, within EX28DRAFT_2865 at 169.291 kb on + strand, within EX28DRAFT_2865 at 169.291 kb on + strand, within EX28DRAFT_2865 at 169.292 kb on - strand, within EX28DRAFT_2865 at 169.292 kb on - strand, within EX28DRAFT_2865 at 169.292 kb on - strand, within EX28DRAFT_2865 at 169.292 kb on - strand, within EX28DRAFT_2865 at 169.704 kb on - strand, within EX28DRAFT_2865 at 170.081 kb on - strand, within EX28DRAFT_2865 at 170.121 kb on - strand, within EX28DRAFT_2865 at 170.121 kb on - strand, within EX28DRAFT_2865 at 170.295 kb on - strand at 170.309 kb on - strand at 170.366 kb on - strand at 170.403 kb on + strand at 170.404 kb on - strand at 170.436 kb on + strand at 170.436 kb on + strand at 170.436 kb on + strand at 170.437 kb on - strand at 170.442 kb on + strand at 170.442 kb on + strand at 170.443 kb on - strand at 170.443 kb on - strand at 170.443 kb on - strand at 170.447 kb on - strand at 170.447 kb on - strand at 170.452 kb on - strand at 170.551 kb on + strand at 170.721 kb on + strand at 170.891 kb on + strand, within EX28DRAFT_2866 at 170.891 kb on + strand, within EX28DRAFT_2866 at 170.892 kb on - strand, within EX28DRAFT_2866 at 170.938 kb on + strand, within EX28DRAFT_2866 at 170.938 kb on + strand, within EX28DRAFT_2866 at 170.938 kb on + strand, within EX28DRAFT_2866 at 170.939 kb on - strand, within EX28DRAFT_2866 at 170.993 kb on + strand, within EX28DRAFT_2866 at 170.994 kb on - strand, within EX28DRAFT_2866 at 170.994 kb on - strand, within EX28DRAFT_2866 at 171.158 kb on - strand, within EX28DRAFT_2866 at 171.370 kb on + strand, within EX28DRAFT_2866
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 168,139 - EX28DRAFT_2864 0.58 +0.7 168,182 + EX28DRAFT_2864 0.60 -0.2 168,183 - EX28DRAFT_2864 0.60 +0.4 168,298 - EX28DRAFT_2864 0.66 +0.2 168,302 - EX28DRAFT_2864 0.66 -1.4 169,080 - -0.3 169,120 - -0.1 169,260 + EX28DRAFT_2865 0.11 +0.4 169,260 + EX28DRAFT_2865 0.11 -1.7 169,260 + EX28DRAFT_2865 0.11 -0.5 169,260 + EX28DRAFT_2865 0.11 -0.6 169,261 - EX28DRAFT_2865 0.11 -0.1 169,285 + EX28DRAFT_2865 0.13 -0.1 169,288 + EX28DRAFT_2865 0.13 -1.6 169,289 - EX28DRAFT_2865 0.13 -0.7 169,289 - EX28DRAFT_2865 0.13 +0.0 169,291 + EX28DRAFT_2865 0.13 -0.3 169,291 + EX28DRAFT_2865 0.13 +0.2 169,291 + EX28DRAFT_2865 0.13 +0.1 169,291 + EX28DRAFT_2865 0.13 +0.3 169,291 + EX28DRAFT_2865 0.13 -0.2 169,292 - EX28DRAFT_2865 0.13 -0.3 169,292 - EX28DRAFT_2865 0.13 +0.8 169,292 - EX28DRAFT_2865 0.13 -0.2 169,292 - EX28DRAFT_2865 0.13 -0.5 169,704 - EX28DRAFT_2865 0.45 -1.5 170,081 - EX28DRAFT_2865 0.74 -1.6 170,121 - EX28DRAFT_2865 0.77 -0.5 170,121 - EX28DRAFT_2865 0.77 -0.1 170,295 - -0.2 170,309 - -0.1 170,366 - +0.3 170,403 + +0.5 170,404 - +0.1 170,436 + -0.3 170,436 + -1.1 170,436 + +0.2 170,437 - +0.6 170,442 + -0.3 170,442 + -0.2 170,443 - +0.3 170,443 - -0.7 170,443 - +0.5 170,447 - +0.3 170,447 - -0.3 170,452 - +0.3 170,551 + +0.2 170,721 + +0.8 170,891 + EX28DRAFT_2866 0.15 +1.3 170,891 + EX28DRAFT_2866 0.15 +0.5 170,892 - EX28DRAFT_2866 0.15 +1.0 170,938 + EX28DRAFT_2866 0.17 +0.6 170,938 + EX28DRAFT_2866 0.17 +0.6 170,938 + EX28DRAFT_2866 0.17 -1.0 170,939 - EX28DRAFT_2866 0.17 +0.8 170,993 + EX28DRAFT_2866 0.19 +0.5 170,994 - EX28DRAFT_2866 0.19 -0.0 170,994 - EX28DRAFT_2866 0.19 +0.7 171,158 - EX28DRAFT_2866 0.25 +0.5 171,370 + EX28DRAFT_2866 0.32 +0.8
Or see this region's nucleotide sequence