Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2228 and EX28DRAFT_2229 are separated by 65 nucleotides EX28DRAFT_2229 and EX28DRAFT_2230 are separated by 0 nucleotides EX28DRAFT_2230 and EX28DRAFT_2231 are separated by 2 nucleotides EX28DRAFT_2231 and EX28DRAFT_2232 overlap by 1 nucleotides
EX28DRAFT_2228: EX28DRAFT_2228 - hypothetical protein, at 2,253,217 to 2,253,561
_2228
EX28DRAFT_2229: EX28DRAFT_2229 - haloacid dehalogenase-like hydrolase, at 2,253,627 to 2,254,172
_2229
EX28DRAFT_2230: EX28DRAFT_2230 - molybdenum ABC transporter, ATP-binding protein, at 2,254,173 to 2,255,231
_2230
EX28DRAFT_2231: EX28DRAFT_2231 - molybdate ABC transporter, permease protein, at 2,255,234 to 2,255,923
_2231
EX28DRAFT_2232: EX28DRAFT_2232 - molybdenum ABC transporter, periplasmic molybdate-binding protein, at 2,255,923 to 2,256,696
_2232
Position (kb)
2254
2255
2256 Strain fitness (log2 ratio)
-2
-1
0
1 at 2253.185 kb on + strand at 2253.218 kb on + strand at 2253.293 kb on + strand, within EX28DRAFT_2228 at 2253.306 kb on + strand, within EX28DRAFT_2228 at 2253.343 kb on + strand, within EX28DRAFT_2228 at 2253.346 kb on - strand, within EX28DRAFT_2228 at 2253.398 kb on + strand, within EX28DRAFT_2228 at 2253.422 kb on + strand, within EX28DRAFT_2228 at 2253.564 kb on + strand at 2253.613 kb on + strand at 2253.710 kb on - strand, within EX28DRAFT_2229 at 2253.728 kb on - strand, within EX28DRAFT_2229 at 2253.860 kb on + strand, within EX28DRAFT_2229 at 2253.972 kb on + strand, within EX28DRAFT_2229 at 2253.973 kb on - strand, within EX28DRAFT_2229 at 2253.973 kb on - strand, within EX28DRAFT_2229 at 2253.973 kb on - strand, within EX28DRAFT_2229 at 2254.026 kb on + strand, within EX28DRAFT_2229 at 2254.026 kb on + strand, within EX28DRAFT_2229 at 2254.155 kb on + strand at 2254.155 kb on + strand at 2254.155 kb on + strand at 2254.156 kb on - strand at 2254.156 kb on - strand at 2254.197 kb on - strand at 2254.314 kb on - strand, within EX28DRAFT_2230 at 2254.349 kb on + strand, within EX28DRAFT_2230 at 2254.448 kb on + strand, within EX28DRAFT_2230 at 2254.449 kb on - strand, within EX28DRAFT_2230 at 2254.495 kb on + strand, within EX28DRAFT_2230 at 2254.681 kb on + strand, within EX28DRAFT_2230 at 2254.682 kb on - strand, within EX28DRAFT_2230 at 2254.698 kb on - strand, within EX28DRAFT_2230 at 2254.720 kb on - strand, within EX28DRAFT_2230 at 2254.720 kb on - strand, within EX28DRAFT_2230 at 2254.720 kb on - strand, within EX28DRAFT_2230 at 2254.723 kb on + strand, within EX28DRAFT_2230 at 2254.724 kb on - strand, within EX28DRAFT_2230 at 2254.783 kb on - strand, within EX28DRAFT_2230 at 2255.235 kb on + strand at 2255.235 kb on + strand at 2255.235 kb on + strand at 2255.236 kb on - strand at 2255.236 kb on - strand at 2255.314 kb on + strand, within EX28DRAFT_2231 at 2255.317 kb on + strand, within EX28DRAFT_2231 at 2255.365 kb on + strand, within EX28DRAFT_2231 at 2255.367 kb on + strand, within EX28DRAFT_2231 at 2255.367 kb on + strand, within EX28DRAFT_2231 at 2255.368 kb on - strand, within EX28DRAFT_2231 at 2255.476 kb on + strand, within EX28DRAFT_2231 at 2255.733 kb on + strand, within EX28DRAFT_2231 at 2255.879 kb on + strand at 2255.880 kb on - strand at 2255.918 kb on + strand at 2255.919 kb on - strand at 2256.012 kb on + strand, within EX28DRAFT_2232 at 2256.038 kb on + strand, within EX28DRAFT_2232 at 2256.085 kb on + strand, within EX28DRAFT_2232 at 2256.085 kb on + strand, within EX28DRAFT_2232 at 2256.094 kb on - strand, within EX28DRAFT_2232
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 2,253,185 + -0.4 2,253,218 + +0.2 2,253,293 + EX28DRAFT_2228 0.22 +0.1 2,253,306 + EX28DRAFT_2228 0.26 -0.2 2,253,343 + EX28DRAFT_2228 0.37 -2.5 2,253,346 - EX28DRAFT_2228 0.37 +0.1 2,253,398 + EX28DRAFT_2228 0.52 -0.5 2,253,422 + EX28DRAFT_2228 0.59 -0.1 2,253,564 + +0.7 2,253,613 + +0.9 2,253,710 - EX28DRAFT_2229 0.15 -0.4 2,253,728 - EX28DRAFT_2229 0.18 +1.2 2,253,860 + EX28DRAFT_2229 0.43 -0.3 2,253,972 + EX28DRAFT_2229 0.63 -0.0 2,253,973 - EX28DRAFT_2229 0.63 -0.4 2,253,973 - EX28DRAFT_2229 0.63 +0.1 2,253,973 - EX28DRAFT_2229 0.63 -0.1 2,254,026 + EX28DRAFT_2229 0.73 -0.1 2,254,026 + EX28DRAFT_2229 0.73 -0.5 2,254,155 + -0.3 2,254,155 + -0.3 2,254,155 + +0.6 2,254,156 - +0.2 2,254,156 - -2.0 2,254,197 - -1.8 2,254,314 - EX28DRAFT_2230 0.13 -0.4 2,254,349 + EX28DRAFT_2230 0.17 +0.4 2,254,448 + EX28DRAFT_2230 0.26 -0.9 2,254,449 - EX28DRAFT_2230 0.26 -0.3 2,254,495 + EX28DRAFT_2230 0.30 +0.0 2,254,681 + EX28DRAFT_2230 0.48 -0.5 2,254,682 - EX28DRAFT_2230 0.48 +0.4 2,254,698 - EX28DRAFT_2230 0.50 -0.4 2,254,720 - EX28DRAFT_2230 0.52 -0.7 2,254,720 - EX28DRAFT_2230 0.52 +1.2 2,254,720 - EX28DRAFT_2230 0.52 -0.1 2,254,723 + EX28DRAFT_2230 0.52 +0.6 2,254,724 - EX28DRAFT_2230 0.52 +0.5 2,254,783 - EX28DRAFT_2230 0.58 +0.1 2,255,235 + +1.7 2,255,235 + +0.1 2,255,235 + -1.0 2,255,236 - +0.2 2,255,236 - +0.2 2,255,314 + EX28DRAFT_2231 0.12 -0.5 2,255,317 + EX28DRAFT_2231 0.12 -0.7 2,255,365 + EX28DRAFT_2231 0.19 +0.2 2,255,367 + EX28DRAFT_2231 0.19 +0.5 2,255,367 + EX28DRAFT_2231 0.19 +0.5 2,255,368 - EX28DRAFT_2231 0.19 +0.5 2,255,476 + EX28DRAFT_2231 0.35 -0.6 2,255,733 + EX28DRAFT_2231 0.72 +0.6 2,255,879 + -0.2 2,255,880 - -0.1 2,255,918 + +0.2 2,255,919 - -0.5 2,256,012 + EX28DRAFT_2232 0.11 +0.0 2,256,038 + EX28DRAFT_2232 0.15 -1.7 2,256,085 + EX28DRAFT_2232 0.21 -1.4 2,256,085 + EX28DRAFT_2232 0.21 +0.2 2,256,094 - EX28DRAFT_2232 0.22 +0.5
Or see this region's nucleotide sequence