Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2227 and EX28DRAFT_2228 are separated by 843 nucleotides EX28DRAFT_2228 and EX28DRAFT_2229 are separated by 65 nucleotides EX28DRAFT_2229 and EX28DRAFT_2230 are separated by 0 nucleotides
EX28DRAFT_2227: EX28DRAFT_2227 - HAD-superfamily hydrolase, subfamily IIB, at 2,251,558 to 2,252,373
_2227
EX28DRAFT_2228: EX28DRAFT_2228 - hypothetical protein, at 2,253,217 to 2,253,561
_2228
EX28DRAFT_2229: EX28DRAFT_2229 - haloacid dehalogenase-like hydrolase, at 2,253,627 to 2,254,172
_2229
EX28DRAFT_2230: EX28DRAFT_2230 - molybdenum ABC transporter, ATP-binding protein, at 2,254,173 to 2,255,231
_2230
Position (kb)
2253
2254 Strain fitness (log2 ratio)
-2
-1
0
1 at 2252.240 kb on + strand, within EX28DRAFT_2227 at 2252.241 kb on - strand, within EX28DRAFT_2227 at 2252.245 kb on - strand, within EX28DRAFT_2227 at 2252.245 kb on - strand, within EX28DRAFT_2227 at 2252.316 kb on - strand at 2252.316 kb on - strand at 2252.325 kb on + strand at 2252.326 kb on - strand at 2252.371 kb on + strand at 2252.376 kb on + strand at 2252.389 kb on - strand at 2252.450 kb on - strand at 2252.523 kb on + strand at 2252.527 kb on + strand at 2252.528 kb on - strand at 2252.554 kb on - strand at 2252.683 kb on - strand at 2253.037 kb on + strand at 2253.082 kb on + strand at 2253.185 kb on + strand at 2253.218 kb on + strand at 2253.293 kb on + strand, within EX28DRAFT_2228 at 2253.306 kb on + strand, within EX28DRAFT_2228 at 2253.343 kb on + strand, within EX28DRAFT_2228 at 2253.346 kb on - strand, within EX28DRAFT_2228 at 2253.398 kb on + strand, within EX28DRAFT_2228 at 2253.422 kb on + strand, within EX28DRAFT_2228 at 2253.564 kb on + strand at 2253.613 kb on + strand at 2253.710 kb on - strand, within EX28DRAFT_2229 at 2253.728 kb on - strand, within EX28DRAFT_2229 at 2253.860 kb on + strand, within EX28DRAFT_2229 at 2253.972 kb on + strand, within EX28DRAFT_2229 at 2253.973 kb on - strand, within EX28DRAFT_2229 at 2253.973 kb on - strand, within EX28DRAFT_2229 at 2253.973 kb on - strand, within EX28DRAFT_2229 at 2254.026 kb on + strand, within EX28DRAFT_2229 at 2254.026 kb on + strand, within EX28DRAFT_2229 at 2254.155 kb on + strand at 2254.155 kb on + strand at 2254.155 kb on + strand at 2254.156 kb on - strand at 2254.156 kb on - strand at 2254.197 kb on - strand at 2254.314 kb on - strand, within EX28DRAFT_2230 at 2254.349 kb on + strand, within EX28DRAFT_2230 at 2254.448 kb on + strand, within EX28DRAFT_2230 at 2254.449 kb on - strand, within EX28DRAFT_2230 at 2254.495 kb on + strand, within EX28DRAFT_2230
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 2,252,240 + EX28DRAFT_2227 0.84 -0.3 2,252,241 - EX28DRAFT_2227 0.84 -0.2 2,252,245 - EX28DRAFT_2227 0.84 -1.6 2,252,245 - EX28DRAFT_2227 0.84 -0.2 2,252,316 - +0.5 2,252,316 - -0.8 2,252,325 + -0.2 2,252,326 - +0.4 2,252,371 + +0.1 2,252,376 + -0.2 2,252,389 - +0.7 2,252,450 - +0.2 2,252,523 + +0.8 2,252,527 + +0.7 2,252,528 - +0.5 2,252,554 - +0.0 2,252,683 - +0.9 2,253,037 + -0.4 2,253,082 + +0.7 2,253,185 + -0.4 2,253,218 + +0.2 2,253,293 + EX28DRAFT_2228 0.22 +0.1 2,253,306 + EX28DRAFT_2228 0.26 -0.2 2,253,343 + EX28DRAFT_2228 0.37 -2.5 2,253,346 - EX28DRAFT_2228 0.37 +0.1 2,253,398 + EX28DRAFT_2228 0.52 -0.5 2,253,422 + EX28DRAFT_2228 0.59 -0.1 2,253,564 + +0.7 2,253,613 + +0.9 2,253,710 - EX28DRAFT_2229 0.15 -0.4 2,253,728 - EX28DRAFT_2229 0.18 +1.2 2,253,860 + EX28DRAFT_2229 0.43 -0.3 2,253,972 + EX28DRAFT_2229 0.63 -0.0 2,253,973 - EX28DRAFT_2229 0.63 -0.4 2,253,973 - EX28DRAFT_2229 0.63 +0.1 2,253,973 - EX28DRAFT_2229 0.63 -0.1 2,254,026 + EX28DRAFT_2229 0.73 -0.1 2,254,026 + EX28DRAFT_2229 0.73 -0.5 2,254,155 + -0.3 2,254,155 + -0.3 2,254,155 + +0.6 2,254,156 - +0.2 2,254,156 - -2.0 2,254,197 - -1.8 2,254,314 - EX28DRAFT_2230 0.13 -0.4 2,254,349 + EX28DRAFT_2230 0.17 +0.4 2,254,448 + EX28DRAFT_2230 0.26 -0.9 2,254,449 - EX28DRAFT_2230 0.26 -0.3 2,254,495 + EX28DRAFT_2230 0.30 +0.0
Or see this region's nucleotide sequence