Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2082 and EX28DRAFT_2083 are separated by 17 nucleotides EX28DRAFT_2083 and EX28DRAFT_2084 are separated by 258 nucleotides EX28DRAFT_2084 and EX28DRAFT_2085 overlap by 4 nucleotides EX28DRAFT_2085 and EX28DRAFT_2086 are separated by 97 nucleotides
EX28DRAFT_2082: EX28DRAFT_2082 - hypothetical protein, at 2,108,509 to 2,109,123
_2082
EX28DRAFT_2083: EX28DRAFT_2083 - hypothetical protein, at 2,109,141 to 2,109,764
_2083
EX28DRAFT_2084: EX28DRAFT_2084 - Negative regulator of beta-lactamase expression, at 2,110,023 to 2,110,853
_2084
EX28DRAFT_2085: EX28DRAFT_2085 - Uncharacterized conserved protein, at 2,110,850 to 2,111,173
_2085
EX28DRAFT_2086: EX28DRAFT_2086 - hypothetical protein, at 2,111,271 to 2,111,789
_2086
Position (kb)
2110
2111 Strain fitness (log2 ratio)
-2
-1
0
1 at 2109.416 kb on + strand, within EX28DRAFT_2083 at 2109.416 kb on + strand, within EX28DRAFT_2083 at 2109.512 kb on - strand, within EX28DRAFT_2083 at 2109.512 kb on - strand, within EX28DRAFT_2083 at 2109.515 kb on + strand, within EX28DRAFT_2083 at 2109.516 kb on - strand, within EX28DRAFT_2083 at 2109.554 kb on + strand, within EX28DRAFT_2083 at 2109.579 kb on - strand, within EX28DRAFT_2083 at 2109.635 kb on + strand, within EX28DRAFT_2083 at 2109.635 kb on + strand, within EX28DRAFT_2083 at 2109.659 kb on + strand, within EX28DRAFT_2083 at 2109.884 kb on - strand at 2109.901 kb on - strand at 2109.952 kb on + strand at 2109.996 kb on + strand at 2109.996 kb on + strand at 2110.334 kb on + strand, within EX28DRAFT_2084 at 2110.397 kb on + strand, within EX28DRAFT_2084 at 2110.639 kb on + strand, within EX28DRAFT_2084 at 2110.702 kb on + strand, within EX28DRAFT_2084 at 2110.722 kb on + strand, within EX28DRAFT_2084 at 2110.722 kb on + strand, within EX28DRAFT_2084 at 2110.723 kb on - strand, within EX28DRAFT_2084 at 2111.125 kb on - strand, within EX28DRAFT_2085 at 2111.133 kb on - strand, within EX28DRAFT_2085 at 2111.234 kb on - strand at 2111.251 kb on + strand at 2111.252 kb on - strand at 2111.258 kb on + strand at 2111.259 kb on - strand at 2111.259 kb on - strand at 2111.418 kb on + strand, within EX28DRAFT_2086 at 2111.418 kb on + strand, within EX28DRAFT_2086 at 2111.530 kb on + strand, within EX28DRAFT_2086 at 2111.531 kb on - strand, within EX28DRAFT_2086
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 2,109,416 + EX28DRAFT_2083 0.44 +0.3 2,109,416 + EX28DRAFT_2083 0.44 -0.4 2,109,512 - EX28DRAFT_2083 0.59 +0.1 2,109,512 - EX28DRAFT_2083 0.59 -1.0 2,109,515 + EX28DRAFT_2083 0.60 +0.3 2,109,516 - EX28DRAFT_2083 0.60 -0.2 2,109,554 + EX28DRAFT_2083 0.66 -0.7 2,109,579 - EX28DRAFT_2083 0.70 -1.7 2,109,635 + EX28DRAFT_2083 0.79 -0.4 2,109,635 + EX28DRAFT_2083 0.79 -1.5 2,109,659 + EX28DRAFT_2083 0.83 +0.7 2,109,884 - +0.4 2,109,901 - -0.3 2,109,952 + +0.1 2,109,996 + +0.8 2,109,996 + +0.1 2,110,334 + EX28DRAFT_2084 0.37 -0.7 2,110,397 + EX28DRAFT_2084 0.45 -0.2 2,110,639 + EX28DRAFT_2084 0.74 -0.0 2,110,702 + EX28DRAFT_2084 0.82 -1.1 2,110,722 + EX28DRAFT_2084 0.84 -1.7 2,110,722 + EX28DRAFT_2084 0.84 -1.8 2,110,723 - EX28DRAFT_2084 0.84 -1.2 2,111,125 - EX28DRAFT_2085 0.85 -0.3 2,111,133 - EX28DRAFT_2085 0.87 -0.4 2,111,234 - +0.4 2,111,251 + +0.8 2,111,252 - -1.6 2,111,258 + +0.0 2,111,259 - +0.8 2,111,259 - -0.4 2,111,418 + EX28DRAFT_2086 0.28 -0.3 2,111,418 + EX28DRAFT_2086 0.28 -0.5 2,111,530 + EX28DRAFT_2086 0.50 +0.3 2,111,531 - EX28DRAFT_2086 0.50 -0.2
Or see this region's nucleotide sequence