Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2069 and EX28DRAFT_2070 overlap by 4 nucleotides EX28DRAFT_2070 and EX28DRAFT_2071 overlap by 4 nucleotides EX28DRAFT_2071 and EX28DRAFT_2072 are separated by 1 nucleotides
EX28DRAFT_2069: EX28DRAFT_2069 - Type II secretory pathway, prepilin signal peptidase PulO and related peptidases, at 2,095,838 to 2,096,629
_2069
EX28DRAFT_2070: EX28DRAFT_2070 - Type II secretory pathway, component PulM, at 2,096,626 to 2,097,111
_2070
EX28DRAFT_2071: EX28DRAFT_2071 - Type II secretory pathway, component PulL, at 2,097,108 to 2,098,262
_2071
EX28DRAFT_2072: EX28DRAFT_2072 - Type II secretory pathway, component PulK, at 2,098,264 to 2,099,283
_2072
Position (kb)
2097
2098
2099 Strain fitness (log2 ratio)
-1
0
1 at 2096.131 kb on - strand, within EX28DRAFT_2069 at 2096.134 kb on + strand, within EX28DRAFT_2069 at 2096.224 kb on + strand, within EX28DRAFT_2069 at 2096.225 kb on - strand, within EX28DRAFT_2069 at 2096.225 kb on - strand, within EX28DRAFT_2069 at 2096.522 kb on - strand, within EX28DRAFT_2069 at 2096.522 kb on - strand, within EX28DRAFT_2069 at 2096.946 kb on + strand, within EX28DRAFT_2070 at 2096.951 kb on - strand, within EX28DRAFT_2070 at 2096.951 kb on - strand, within EX28DRAFT_2070 at 2097.115 kb on + strand at 2097.115 kb on + strand at 2097.139 kb on + strand at 2097.139 kb on + strand at 2097.221 kb on + strand at 2097.376 kb on + strand, within EX28DRAFT_2071 at 2097.376 kb on + strand, within EX28DRAFT_2071 at 2097.377 kb on - strand, within EX28DRAFT_2071 at 2097.379 kb on + strand, within EX28DRAFT_2071 at 2097.379 kb on + strand, within EX28DRAFT_2071 at 2097.415 kb on + strand, within EX28DRAFT_2071 at 2097.415 kb on + strand, within EX28DRAFT_2071 at 2097.483 kb on - strand, within EX28DRAFT_2071 at 2097.678 kb on + strand, within EX28DRAFT_2071 at 2097.797 kb on - strand, within EX28DRAFT_2071 at 2097.821 kb on + strand, within EX28DRAFT_2071 at 2097.822 kb on - strand, within EX28DRAFT_2071 at 2098.168 kb on + strand at 2098.169 kb on - strand at 2098.255 kb on - strand at 2098.265 kb on + strand at 2098.265 kb on + strand at 2098.593 kb on + strand, within EX28DRAFT_2072 at 2098.594 kb on - strand, within EX28DRAFT_2072 at 2098.603 kb on + strand, within EX28DRAFT_2072 at 2098.603 kb on + strand, within EX28DRAFT_2072 at 2098.799 kb on + strand, within EX28DRAFT_2072 at 2098.818 kb on - strand, within EX28DRAFT_2072 at 2098.818 kb on - strand, within EX28DRAFT_2072 at 2099.003 kb on + strand, within EX28DRAFT_2072 at 2099.003 kb on + strand, within EX28DRAFT_2072 at 2099.003 kb on + strand, within EX28DRAFT_2072 at 2099.004 kb on - strand, within EX28DRAFT_2072 at 2099.004 kb on - strand, within EX28DRAFT_2072 at 2099.004 kb on - strand, within EX28DRAFT_2072 at 2099.007 kb on + strand, within EX28DRAFT_2072 at 2099.154 kb on - strand, within EX28DRAFT_2072
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 2,096,131 - EX28DRAFT_2069 0.37 -0.2 2,096,134 + EX28DRAFT_2069 0.37 +0.2 2,096,224 + EX28DRAFT_2069 0.49 +0.7 2,096,225 - EX28DRAFT_2069 0.49 +0.6 2,096,225 - EX28DRAFT_2069 0.49 +1.0 2,096,522 - EX28DRAFT_2069 0.86 -0.5 2,096,522 - EX28DRAFT_2069 0.86 +1.1 2,096,946 + EX28DRAFT_2070 0.66 -0.6 2,096,951 - EX28DRAFT_2070 0.67 +0.1 2,096,951 - EX28DRAFT_2070 0.67 -0.7 2,097,115 + -0.3 2,097,115 + -0.0 2,097,139 + +0.5 2,097,139 + -0.7 2,097,221 + +1.5 2,097,376 + EX28DRAFT_2071 0.23 +0.4 2,097,376 + EX28DRAFT_2071 0.23 +0.3 2,097,377 - EX28DRAFT_2071 0.23 -0.1 2,097,379 + EX28DRAFT_2071 0.23 +1.0 2,097,379 + EX28DRAFT_2071 0.23 +0.1 2,097,415 + EX28DRAFT_2071 0.27 +1.1 2,097,415 + EX28DRAFT_2071 0.27 +0.4 2,097,483 - EX28DRAFT_2071 0.32 +0.3 2,097,678 + EX28DRAFT_2071 0.49 +1.3 2,097,797 - EX28DRAFT_2071 0.60 +0.0 2,097,821 + EX28DRAFT_2071 0.62 -0.5 2,097,822 - EX28DRAFT_2071 0.62 +0.1 2,098,168 + +0.6 2,098,169 - -0.9 2,098,255 - -1.2 2,098,265 + +0.1 2,098,265 + +0.1 2,098,593 + EX28DRAFT_2072 0.32 -0.0 2,098,594 - EX28DRAFT_2072 0.32 +1.6 2,098,603 + EX28DRAFT_2072 0.33 +0.6 2,098,603 + EX28DRAFT_2072 0.33 -0.7 2,098,799 + EX28DRAFT_2072 0.52 -0.5 2,098,818 - EX28DRAFT_2072 0.54 +0.2 2,098,818 - EX28DRAFT_2072 0.54 -0.0 2,099,003 + EX28DRAFT_2072 0.72 -0.1 2,099,003 + EX28DRAFT_2072 0.72 +0.7 2,099,003 + EX28DRAFT_2072 0.72 -0.2 2,099,004 - EX28DRAFT_2072 0.73 +0.2 2,099,004 - EX28DRAFT_2072 0.73 +0.1 2,099,004 - EX28DRAFT_2072 0.73 -0.0 2,099,007 + EX28DRAFT_2072 0.73 -0.4 2,099,154 - EX28DRAFT_2072 0.87 +0.8
Or see this region's nucleotide sequence