Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1295 and EX28DRAFT_1296 overlap by 4 nucleotides EX28DRAFT_1296 and EX28DRAFT_1297 overlap by 14 nucleotides EX28DRAFT_1297 and EX28DRAFT_1298 are separated by 245 nucleotides EX28DRAFT_1298 and EX28DRAFT_1299 are separated by 82 nucleotides EX28DRAFT_1299 and EX28DRAFT_1300 overlap by 4 nucleotides EX28DRAFT_1300 and EX28DRAFT_1301 overlap by 4 nucleotides
EX28DRAFT_1295: EX28DRAFT_1295 - Protein of unknown function (DUF2570), at 1,307,769 to 1,308,155
_1295
EX28DRAFT_1296: EX28DRAFT_1296 - Phage-related lysozyme (muraminidase), at 1,308,152 to 1,308,592
_1296
EX28DRAFT_1297: EX28DRAFT_1297 - hypothetical protein, at 1,308,579 to 1,308,896
_1297
EX28DRAFT_1298: EX28DRAFT_1298 - hypothetical protein, at 1,309,142 to 1,309,351
_1298
EX28DRAFT_1299: EX28DRAFT_1299 - Phage antitermination protein Q, at 1,309,434 to 1,310,123
_1299
EX28DRAFT_1300: EX28DRAFT_1300 - hypothetical protein, at 1,310,120 to 1,310,260
_1300
EX28DRAFT_1301: EX28DRAFT_1301 - hypothetical protein, at 1,310,257 to 1,310,481
_1301
Position (kb)
1309
1310 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1308.258 kb on + strand, within EX28DRAFT_1296 at 1308.259 kb on - strand, within EX28DRAFT_1296 at 1308.320 kb on - strand, within EX28DRAFT_1296 at 1308.321 kb on + strand, within EX28DRAFT_1296 at 1308.321 kb on + strand, within EX28DRAFT_1296 at 1308.321 kb on + strand, within EX28DRAFT_1296 at 1308.322 kb on - strand, within EX28DRAFT_1296 at 1308.322 kb on - strand, within EX28DRAFT_1296 at 1308.515 kb on + strand, within EX28DRAFT_1296 at 1308.515 kb on + strand, within EX28DRAFT_1296 at 1308.515 kb on + strand, within EX28DRAFT_1296 at 1308.622 kb on + strand, within EX28DRAFT_1297 at 1308.623 kb on - strand, within EX28DRAFT_1297 at 1308.644 kb on + strand, within EX28DRAFT_1297 at 1308.679 kb on + strand, within EX28DRAFT_1297 at 1308.729 kb on - strand, within EX28DRAFT_1297 at 1308.801 kb on - strand, within EX28DRAFT_1297 at 1308.805 kb on + strand, within EX28DRAFT_1297 at 1308.875 kb on + strand at 1308.884 kb on + strand at 1308.885 kb on - strand at 1308.896 kb on + strand at 1308.897 kb on - strand at 1308.957 kb on + strand at 1308.957 kb on + strand at 1308.957 kb on + strand at 1308.957 kb on + strand at 1308.957 kb on + strand at 1308.958 kb on - strand at 1308.958 kb on - strand at 1308.958 kb on - strand at 1308.958 kb on - strand at 1308.994 kb on - strand at 1309.094 kb on + strand at 1309.143 kb on + strand at 1309.151 kb on + strand at 1309.151 kb on + strand at 1309.190 kb on - strand, within EX28DRAFT_1298 at 1309.308 kb on - strand, within EX28DRAFT_1298 at 1309.324 kb on + strand, within EX28DRAFT_1298 at 1309.325 kb on - strand, within EX28DRAFT_1298 at 1309.325 kb on - strand, within EX28DRAFT_1298 at 1309.343 kb on + strand at 1309.619 kb on - strand, within EX28DRAFT_1299 at 1309.676 kb on + strand, within EX28DRAFT_1299 at 1309.677 kb on - strand, within EX28DRAFT_1299 at 1309.798 kb on + strand, within EX28DRAFT_1299 at 1309.799 kb on - strand, within EX28DRAFT_1299 at 1309.799 kb on - strand, within EX28DRAFT_1299 at 1309.965 kb on - strand, within EX28DRAFT_1299 at 1310.037 kb on + strand, within EX28DRAFT_1299 at 1310.101 kb on + strand at 1310.146 kb on - strand, within EX28DRAFT_1300 at 1310.238 kb on - strand, within EX28DRAFT_1300 at 1310.285 kb on + strand, within EX28DRAFT_1301 at 1310.286 kb on - strand, within EX28DRAFT_1301 at 1310.286 kb on - strand, within EX28DRAFT_1301 at 1310.286 kb on - strand, within EX28DRAFT_1301 at 1310.294 kb on + strand, within EX28DRAFT_1301
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 1,308,258 + EX28DRAFT_1296 0.24 -0.0 1,308,259 - EX28DRAFT_1296 0.24 +0.8 1,308,320 - EX28DRAFT_1296 0.38 +0.2 1,308,321 + EX28DRAFT_1296 0.38 +2.8 1,308,321 + EX28DRAFT_1296 0.38 -0.4 1,308,321 + EX28DRAFT_1296 0.38 -0.9 1,308,322 - EX28DRAFT_1296 0.39 -0.4 1,308,322 - EX28DRAFT_1296 0.39 +1.8 1,308,515 + EX28DRAFT_1296 0.82 +0.3 1,308,515 + EX28DRAFT_1296 0.82 -0.6 1,308,515 + EX28DRAFT_1296 0.82 +0.1 1,308,622 + EX28DRAFT_1297 0.14 +0.1 1,308,623 - EX28DRAFT_1297 0.14 +0.7 1,308,644 + EX28DRAFT_1297 0.20 +1.6 1,308,679 + EX28DRAFT_1297 0.31 -0.1 1,308,729 - EX28DRAFT_1297 0.47 -0.2 1,308,801 - EX28DRAFT_1297 0.70 +0.6 1,308,805 + EX28DRAFT_1297 0.71 +0.7 1,308,875 + +1.6 1,308,884 + -0.3 1,308,885 - -0.1 1,308,896 + +0.3 1,308,897 - +0.0 1,308,957 + +0.3 1,308,957 + +0.6 1,308,957 + -0.8 1,308,957 + -0.4 1,308,957 + -1.0 1,308,958 - +0.2 1,308,958 - -0.4 1,308,958 - +0.4 1,308,958 - +0.2 1,308,994 - +0.0 1,309,094 + +0.5 1,309,143 + +1.8 1,309,151 + +0.3 1,309,151 + +0.2 1,309,190 - EX28DRAFT_1298 0.23 -0.8 1,309,308 - EX28DRAFT_1298 0.79 -0.5 1,309,324 + EX28DRAFT_1298 0.87 -0.2 1,309,325 - EX28DRAFT_1298 0.87 -0.1 1,309,325 - EX28DRAFT_1298 0.87 -1.6 1,309,343 + +0.0 1,309,619 - EX28DRAFT_1299 0.27 +0.1 1,309,676 + EX28DRAFT_1299 0.35 -0.1 1,309,677 - EX28DRAFT_1299 0.35 -0.6 1,309,798 + EX28DRAFT_1299 0.53 +0.2 1,309,799 - EX28DRAFT_1299 0.53 -0.5 1,309,799 - EX28DRAFT_1299 0.53 +0.4 1,309,965 - EX28DRAFT_1299 0.77 -0.2 1,310,037 + EX28DRAFT_1299 0.87 -0.0 1,310,101 + +0.2 1,310,146 - EX28DRAFT_1300 0.18 -0.8 1,310,238 - EX28DRAFT_1300 0.84 +0.1 1,310,285 + EX28DRAFT_1301 0.12 -0.3 1,310,286 - EX28DRAFT_1301 0.13 -0.2 1,310,286 - EX28DRAFT_1301 0.13 -0.2 1,310,286 - EX28DRAFT_1301 0.13 -0.4 1,310,294 + EX28DRAFT_1301 0.16 +0.4
Or see this region's nucleotide sequence