Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0654 and EX28DRAFT_0655 are separated by 176 nucleotides EX28DRAFT_0655 and EX28DRAFT_0656 overlap by 26 nucleotides EX28DRAFT_0656 and EX28DRAFT_0657 are separated by 15 nucleotides
EX28DRAFT_0654: EX28DRAFT_0654 - Ribose/xylose/arabinose/galactoside ABC-type transport systems, permease components, at 648,548 to 649,534
_0654
EX28DRAFT_0655: EX28DRAFT_0655 - trehalose-phosphatase, at 649,711 to 650,514
_0655
EX28DRAFT_0656: EX28DRAFT_0656 - alpha,alpha-trehalose-phosphate synthase [UDP-forming], at 650,489 to 651,913
_0656
EX28DRAFT_0657: EX28DRAFT_0657 - Universal stress protein UspA and related nucleotide-binding proteins, at 651,929 to 652,357
_0657
Position (kb)
650
651
652 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 649.565 kb on - strand at 649.572 kb on + strand at 649.572 kb on + strand at 649.572 kb on + strand at 649.647 kb on - strand at 649.649 kb on - strand at 649.649 kb on - strand at 649.652 kb on + strand at 649.652 kb on + strand at 649.652 kb on + strand at 649.652 kb on + strand at 649.753 kb on + strand at 649.754 kb on - strand at 649.754 kb on - strand at 649.819 kb on - strand, within EX28DRAFT_0655 at 649.992 kb on + strand, within EX28DRAFT_0655 at 650.213 kb on + strand, within EX28DRAFT_0655 at 650.214 kb on - strand, within EX28DRAFT_0655 at 650.232 kb on + strand, within EX28DRAFT_0655 at 650.430 kb on - strand, within EX28DRAFT_0655 at 650.462 kb on + strand at 650.463 kb on - strand at 650.499 kb on + strand at 650.574 kb on + strand at 650.574 kb on + strand at 650.575 kb on - strand at 650.575 kb on - strand at 650.575 kb on - strand at 650.575 kb on - strand at 650.575 kb on - strand at 650.671 kb on + strand, within EX28DRAFT_0656 at 650.671 kb on + strand, within EX28DRAFT_0656 at 650.705 kb on + strand, within EX28DRAFT_0656 at 650.714 kb on + strand, within EX28DRAFT_0656 at 650.717 kb on + strand, within EX28DRAFT_0656 at 650.718 kb on - strand, within EX28DRAFT_0656 at 650.760 kb on - strand, within EX28DRAFT_0656 at 651.088 kb on + strand, within EX28DRAFT_0656 at 651.089 kb on - strand, within EX28DRAFT_0656 at 651.233 kb on - strand, within EX28DRAFT_0656 at 651.600 kb on - strand, within EX28DRAFT_0656 at 651.760 kb on + strand, within EX28DRAFT_0656 at 651.805 kb on + strand at 651.841 kb on - strand at 651.841 kb on - strand at 651.916 kb on + strand at 651.982 kb on + strand, within EX28DRAFT_0657 at 652.044 kb on - strand, within EX28DRAFT_0657 at 652.044 kb on - strand, within EX28DRAFT_0657 at 652.069 kb on + strand, within EX28DRAFT_0657 at 652.079 kb on - strand, within EX28DRAFT_0657 at 652.116 kb on + strand, within EX28DRAFT_0657 at 652.197 kb on - strand, within EX28DRAFT_0657 at 652.220 kb on - strand, within EX28DRAFT_0657 at 652.221 kb on + strand, within EX28DRAFT_0657 at 652.221 kb on + strand, within EX28DRAFT_0657 at 652.222 kb on - strand, within EX28DRAFT_0657 at 652.222 kb on - strand, within EX28DRAFT_0657 at 652.222 kb on - strand, within EX28DRAFT_0657 at 652.442 kb on + strand at 652.474 kb on + strand at 652.641 kb on + strand at 652.643 kb on + strand at 652.691 kb on + strand at 652.692 kb on - strand at 652.775 kb on + strand at 652.909 kb on + strand at 652.909 kb on + strand at 652.909 kb on + strand at 652.909 kb on + strand at 652.909 kb on + strand at 652.909 kb on + strand at 652.910 kb on - strand at 652.910 kb on - strand at 652.910 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 649,565 - +0.6 649,572 + +0.9 649,572 + -0.5 649,572 + +0.4 649,647 - +0.2 649,649 - -0.0 649,649 - -0.1 649,652 + +0.4 649,652 + -0.8 649,652 + +0.6 649,652 + -0.3 649,753 + -0.3 649,754 - +0.5 649,754 - +1.6 649,819 - EX28DRAFT_0655 0.13 +0.0 649,992 + EX28DRAFT_0655 0.35 +0.4 650,213 + EX28DRAFT_0655 0.62 +0.1 650,214 - EX28DRAFT_0655 0.63 +1.2 650,232 + EX28DRAFT_0655 0.65 -0.7 650,430 - EX28DRAFT_0655 0.89 +1.4 650,462 + -0.6 650,463 - -0.4 650,499 + -0.2 650,574 + +0.1 650,574 + +1.7 650,575 - -0.3 650,575 - +0.2 650,575 - +0.2 650,575 - -0.3 650,575 - +0.9 650,671 + EX28DRAFT_0656 0.13 -0.5 650,671 + EX28DRAFT_0656 0.13 -0.7 650,705 + EX28DRAFT_0656 0.15 +0.7 650,714 + EX28DRAFT_0656 0.16 -0.2 650,717 + EX28DRAFT_0656 0.16 -0.2 650,718 - EX28DRAFT_0656 0.16 +0.3 650,760 - EX28DRAFT_0656 0.19 -0.4 651,088 + EX28DRAFT_0656 0.42 -0.2 651,089 - EX28DRAFT_0656 0.42 +0.8 651,233 - EX28DRAFT_0656 0.52 -0.5 651,600 - EX28DRAFT_0656 0.78 -1.4 651,760 + EX28DRAFT_0656 0.89 -0.1 651,805 + +0.3 651,841 - -1.3 651,841 - -0.6 651,916 + -0.8 651,982 + EX28DRAFT_0657 0.12 -0.2 652,044 - EX28DRAFT_0657 0.27 -0.9 652,044 - EX28DRAFT_0657 0.27 -0.9 652,069 + EX28DRAFT_0657 0.33 -0.4 652,079 - EX28DRAFT_0657 0.35 +0.6 652,116 + EX28DRAFT_0657 0.44 +0.4 652,197 - EX28DRAFT_0657 0.62 +0.6 652,220 - EX28DRAFT_0657 0.68 +0.2 652,221 + EX28DRAFT_0657 0.68 +0.9 652,221 + EX28DRAFT_0657 0.68 -1.4 652,222 - EX28DRAFT_0657 0.68 -0.6 652,222 - EX28DRAFT_0657 0.68 -3.4 652,222 - EX28DRAFT_0657 0.68 -0.3 652,442 + -1.0 652,474 + -0.8 652,641 + -1.3 652,643 + +0.6 652,691 + -0.6 652,692 - -0.1 652,775 + -1.0 652,909 + +0.0 652,909 + +1.0 652,909 + +0.2 652,909 + +0.2 652,909 + +0.2 652,909 + +0.2 652,910 - -0.2 652,910 - +0.3 652,910 - -0.1
Or see this region's nucleotide sequence