Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0502 and EX28DRAFT_0503 are separated by 2 nucleotides EX28DRAFT_0503 and EX28DRAFT_0504 are separated by 2 nucleotides EX28DRAFT_0504 and EX28DRAFT_0505 overlap by 4 nucleotides
EX28DRAFT_0502: EX28DRAFT_0502 - GDP-mannose 4,6-dehydratase, at 493,466 to 494,587
_0502
EX28DRAFT_0503: EX28DRAFT_0503 - Nucleoside-diphosphate-sugar epimerases, at 494,590 to 495,555
_0503
EX28DRAFT_0504: EX28DRAFT_0504 - ADP-ribose pyrophosphatase, at 495,558 to 496,037
_0504
EX28DRAFT_0505: EX28DRAFT_0505 - colanic acid biosynthesis glycosyl transferase WcaI, at 496,034 to 497,257
_0505
Position (kb) 495
496
497 Strain fitness (log2 ratio) -1
0
1
2 at 494.605 kb on - strand at 494.768 kb on - strand, within EX28DRAFT_0503 at 494.768 kb on - strand, within EX28DRAFT_0503 at 494.889 kb on + strand, within EX28DRAFT_0503 at 494.996 kb on - strand, within EX28DRAFT_0503 at 495.049 kb on + strand, within EX28DRAFT_0503 at 495.378 kb on - strand, within EX28DRAFT_0503 at 495.511 kb on - strand at 495.514 kb on + strand at 495.514 kb on + strand at 495.515 kb on - strand at 495.589 kb on + strand at 495.822 kb on + strand, within EX28DRAFT_0504 at 495.823 kb on - strand, within EX28DRAFT_0504 at 495.830 kb on + strand, within EX28DRAFT_0504 at 495.861 kb on + strand, within EX28DRAFT_0504 at 495.862 kb on - strand, within EX28DRAFT_0504 at 495.997 kb on - strand at 496.033 kb on + strand at 496.034 kb on - strand at 496.048 kb on - strand at 496.061 kb on + strand at 496.062 kb on - strand at 496.250 kb on + strand, within EX28DRAFT_0505 at 496.441 kb on + strand, within EX28DRAFT_0505 at 496.441 kb on + strand, within EX28DRAFT_0505 at 496.452 kb on - strand, within EX28DRAFT_0505 at 496.560 kb on - strand, within EX28DRAFT_0505 at 496.917 kb on - strand, within EX28DRAFT_0505
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 494,605 - -1.3 494,768 - EX28DRAFT_0503 0.18 -0.3 494,768 - EX28DRAFT_0503 0.18 +0.5 494,889 + EX28DRAFT_0503 0.31 -0.0 494,996 - EX28DRAFT_0503 0.42 +0.1 495,049 + EX28DRAFT_0503 0.48 +0.3 495,378 - EX28DRAFT_0503 0.82 +0.9 495,511 - -0.7 495,514 + -0.2 495,514 + -0.1 495,515 - -0.6 495,589 + +0.2 495,822 + EX28DRAFT_0504 0.55 +0.9 495,823 - EX28DRAFT_0504 0.55 +2.5 495,830 + EX28DRAFT_0504 0.57 +0.3 495,861 + EX28DRAFT_0504 0.63 -0.3 495,862 - EX28DRAFT_0504 0.63 +0.7 495,997 - +0.1 496,033 + -0.2 496,034 - -0.1 496,048 - +0.0 496,061 + -1.3 496,062 - +0.0 496,250 + EX28DRAFT_0505 0.18 +0.4 496,441 + EX28DRAFT_0505 0.33 +2.0 496,441 + EX28DRAFT_0505 0.33 -1.1 496,452 - EX28DRAFT_0505 0.34 -1.5 496,560 - EX28DRAFT_0505 0.43 +0.3 496,917 - EX28DRAFT_0505 0.72 +1.0
Or see this region's nucleotide sequence