Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0493 and EX28DRAFT_0494 are separated by 6 nucleotides EX28DRAFT_0494 and EX28DRAFT_0495 are separated by 2 nucleotides EX28DRAFT_0495 and EX28DRAFT_0496 are separated by 74 nucleotides EX28DRAFT_0496 and EX28DRAFT_0497 overlap by 1 nucleotides
EX28DRAFT_0493: EX28DRAFT_0493 - Periplasmic protein involved in polysaccharide export, at 484,522 to 485,661
_0493
EX28DRAFT_0494: EX28DRAFT_0494 - Protein-tyrosine-phosphatase, at 485,668 to 486,111
_0494
EX28DRAFT_0495: EX28DRAFT_0495 - capsular exopolysaccharide family, at 486,114 to 488,276
_0495
EX28DRAFT_0496: EX28DRAFT_0496 - colanic acid biosynthesis glycosyl transferase WcaA, at 488,351 to 489,214
_0496
EX28DRAFT_0497: EX28DRAFT_0497 - colanic acid biosynthesis acetyltransferase WcaB, at 489,214 to 489,705
_0497
Position (kb) 486
487
488
489 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 485.191 kb on - strand, within EX28DRAFT_0493 at 485.191 kb on - strand, within EX28DRAFT_0493 at 485.484 kb on + strand, within EX28DRAFT_0493 at 485.485 kb on - strand, within EX28DRAFT_0493 at 485.537 kb on - strand, within EX28DRAFT_0493 at 485.548 kb on + strand at 485.548 kb on + strand at 485.652 kb on + strand at 485.653 kb on - strand at 485.690 kb on + strand at 486.049 kb on + strand, within EX28DRAFT_0494 at 486.057 kb on - strand, within EX28DRAFT_0494 at 486.255 kb on + strand at 486.257 kb on + strand at 486.444 kb on - strand, within EX28DRAFT_0495 at 486.461 kb on + strand, within EX28DRAFT_0495 at 486.828 kb on - strand, within EX28DRAFT_0495 at 486.870 kb on + strand, within EX28DRAFT_0495 at 486.870 kb on + strand, within EX28DRAFT_0495 at 486.894 kb on - strand, within EX28DRAFT_0495 at 487.269 kb on + strand, within EX28DRAFT_0495 at 487.270 kb on - strand, within EX28DRAFT_0495 at 487.395 kb on - strand, within EX28DRAFT_0495 at 487.705 kb on + strand, within EX28DRAFT_0495 at 487.818 kb on + strand, within EX28DRAFT_0495 at 487.819 kb on - strand, within EX28DRAFT_0495 at 487.908 kb on - strand, within EX28DRAFT_0495 at 488.244 kb on + strand at 488.256 kb on + strand at 488.259 kb on - strand at 488.274 kb on + strand at 488.402 kb on + strand at 488.403 kb on - strand at 488.565 kb on - strand, within EX28DRAFT_0496 at 488.565 kb on - strand, within EX28DRAFT_0496 at 488.582 kb on - strand, within EX28DRAFT_0496 at 488.617 kb on + strand, within EX28DRAFT_0496 at 488.618 kb on - strand, within EX28DRAFT_0496 at 488.732 kb on + strand, within EX28DRAFT_0496 at 488.744 kb on + strand, within EX28DRAFT_0496 at 488.744 kb on + strand, within EX28DRAFT_0496 at 488.745 kb on - strand, within EX28DRAFT_0496 at 488.745 kb on - strand, within EX28DRAFT_0496 at 488.745 kb on - strand, within EX28DRAFT_0496 at 488.808 kb on - strand, within EX28DRAFT_0496 at 488.825 kb on + strand, within EX28DRAFT_0496 at 488.825 kb on + strand, within EX28DRAFT_0496 at 488.919 kb on - strand, within EX28DRAFT_0496 at 488.949 kb on - strand, within EX28DRAFT_0496 at 489.094 kb on + strand, within EX28DRAFT_0496 at 489.130 kb on + strand at 489.130 kb on + strand at 489.212 kb on + strand at 489.212 kb on + strand at 489.213 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 485,191 - EX28DRAFT_0493 0.59 +0.3 485,191 - EX28DRAFT_0493 0.59 +0.6 485,484 + EX28DRAFT_0493 0.84 +0.9 485,485 - EX28DRAFT_0493 0.84 +0.1 485,537 - EX28DRAFT_0493 0.89 -0.6 485,548 + -0.4 485,548 + -0.3 485,652 + -0.3 485,653 - -0.3 485,690 + +0.5 486,049 + EX28DRAFT_0494 0.86 -3.9 486,057 - EX28DRAFT_0494 0.88 -0.6 486,255 + -0.0 486,257 + +1.5 486,444 - EX28DRAFT_0495 0.15 +0.3 486,461 + EX28DRAFT_0495 0.16 +0.1 486,828 - EX28DRAFT_0495 0.33 -0.2 486,870 + EX28DRAFT_0495 0.35 -0.3 486,870 + EX28DRAFT_0495 0.35 +1.4 486,894 - EX28DRAFT_0495 0.36 -1.3 487,269 + EX28DRAFT_0495 0.53 +0.3 487,270 - EX28DRAFT_0495 0.53 -1.3 487,395 - EX28DRAFT_0495 0.59 +0.1 487,705 + EX28DRAFT_0495 0.74 +0.2 487,818 + EX28DRAFT_0495 0.79 -1.5 487,819 - EX28DRAFT_0495 0.79 -1.1 487,908 - EX28DRAFT_0495 0.83 -1.5 488,244 + -0.1 488,256 + -0.0 488,259 - -0.4 488,274 + +2.2 488,402 + -0.2 488,403 - +0.2 488,565 - EX28DRAFT_0496 0.25 +1.1 488,565 - EX28DRAFT_0496 0.25 +0.1 488,582 - EX28DRAFT_0496 0.27 +0.4 488,617 + EX28DRAFT_0496 0.31 -0.1 488,618 - EX28DRAFT_0496 0.31 +1.3 488,732 + EX28DRAFT_0496 0.44 +0.6 488,744 + EX28DRAFT_0496 0.45 +0.5 488,744 + EX28DRAFT_0496 0.45 +1.1 488,745 - EX28DRAFT_0496 0.46 -0.9 488,745 - EX28DRAFT_0496 0.46 -0.2 488,745 - EX28DRAFT_0496 0.46 -0.9 488,808 - EX28DRAFT_0496 0.53 -0.2 488,825 + EX28DRAFT_0496 0.55 -0.1 488,825 + EX28DRAFT_0496 0.55 +0.3 488,919 - EX28DRAFT_0496 0.66 +1.3 488,949 - EX28DRAFT_0496 0.69 -0.7 489,094 + EX28DRAFT_0496 0.86 -0.1 489,130 + +0.0 489,130 + +0.3 489,212 + +0.3 489,212 + -0.9 489,213 - +0.1
Or see this region's nucleotide sequence