Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0421 and EX28DRAFT_0422 are separated by 326 nucleotides EX28DRAFT_0422 and EX28DRAFT_0423 are separated by 50 nucleotides EX28DRAFT_0423 and EX28DRAFT_0424 are separated by 84 nucleotides
EX28DRAFT_0421: EX28DRAFT_0421 - Beta-glucosidase/6-phospho-beta-glucosidase/beta-galactosidase, at 399,899 to 401,293
_0421
EX28DRAFT_0422: EX28DRAFT_0422 - efflux transporter, outer membrane factor (OMF) lipoprotein, NodT family, at 401,620 to 403,038
_0422
EX28DRAFT_0423: EX28DRAFT_0423 - Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases), at 403,089 to 403,853
_0423
EX28DRAFT_0424: EX28DRAFT_0424 - Uncharacterized membrane-associated protein, at 403,938 to 404,510
_0424
Position (kb) 401
402
403
404 Strain fitness (log2 ratio) -2
-1
0
1
2 at 400.621 kb on - strand, within EX28DRAFT_0421 at 400.695 kb on - strand, within EX28DRAFT_0421 at 400.696 kb on + strand, within EX28DRAFT_0421 at 400.697 kb on - strand, within EX28DRAFT_0421 at 400.797 kb on - strand, within EX28DRAFT_0421 at 400.797 kb on - strand, within EX28DRAFT_0421 at 401.045 kb on - strand, within EX28DRAFT_0421 at 401.048 kb on + strand, within EX28DRAFT_0421 at 401.049 kb on - strand, within EX28DRAFT_0421 at 401.111 kb on + strand, within EX28DRAFT_0421 at 401.114 kb on - strand, within EX28DRAFT_0421 at 401.183 kb on + strand at 401.243 kb on + strand at 401.244 kb on - strand at 401.249 kb on + strand at 401.250 kb on - strand at 401.330 kb on - strand at 401.436 kb on - strand at 401.664 kb on + strand at 401.868 kb on + strand, within EX28DRAFT_0422 at 401.869 kb on - strand, within EX28DRAFT_0422 at 401.869 kb on - strand, within EX28DRAFT_0422 at 402.178 kb on + strand, within EX28DRAFT_0422 at 402.179 kb on - strand, within EX28DRAFT_0422 at 402.248 kb on + strand, within EX28DRAFT_0422 at 402.249 kb on - strand, within EX28DRAFT_0422 at 402.249 kb on - strand, within EX28DRAFT_0422 at 402.271 kb on + strand, within EX28DRAFT_0422 at 402.374 kb on + strand, within EX28DRAFT_0422 at 402.420 kb on + strand, within EX28DRAFT_0422 at 402.517 kb on + strand, within EX28DRAFT_0422 at 402.518 kb on - strand, within EX28DRAFT_0422 at 402.565 kb on + strand, within EX28DRAFT_0422 at 402.566 kb on - strand, within EX28DRAFT_0422 at 402.729 kb on + strand, within EX28DRAFT_0422 at 402.729 kb on + strand, within EX28DRAFT_0422 at 402.730 kb on - strand, within EX28DRAFT_0422 at 402.730 kb on - strand, within EX28DRAFT_0422 at 402.749 kb on - strand, within EX28DRAFT_0422 at 402.980 kb on - strand at 403.049 kb on + strand at 403.049 kb on + strand at 403.053 kb on + strand at 403.054 kb on - strand at 403.055 kb on + strand at 403.055 kb on + strand at 403.056 kb on - strand at 403.056 kb on - strand at 403.056 kb on - strand at 403.060 kb on - strand at 403.060 kb on - strand at 403.060 kb on - strand at 403.271 kb on - strand, within EX28DRAFT_0423 at 403.271 kb on - strand, within EX28DRAFT_0423 at 403.799 kb on + strand at 403.852 kb on - strand at 403.911 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 400,621 - EX28DRAFT_0421 0.52 +0.1 400,695 - EX28DRAFT_0421 0.57 -1.5 400,696 + EX28DRAFT_0421 0.57 +0.1 400,697 - EX28DRAFT_0421 0.57 -0.9 400,797 - EX28DRAFT_0421 0.64 +2.1 400,797 - EX28DRAFT_0421 0.64 -0.3 401,045 - EX28DRAFT_0421 0.82 -0.2 401,048 + EX28DRAFT_0421 0.82 -0.7 401,049 - EX28DRAFT_0421 0.82 -0.3 401,111 + EX28DRAFT_0421 0.87 +0.5 401,114 - EX28DRAFT_0421 0.87 -2.2 401,183 + +0.3 401,243 + -0.1 401,244 - +0.1 401,249 + +0.7 401,250 - +0.3 401,330 - -0.1 401,436 - +0.7 401,664 + -0.6 401,868 + EX28DRAFT_0422 0.17 -0.6 401,869 - EX28DRAFT_0422 0.18 -1.4 401,869 - EX28DRAFT_0422 0.18 -0.2 402,178 + EX28DRAFT_0422 0.39 -0.4 402,179 - EX28DRAFT_0422 0.39 +0.3 402,248 + EX28DRAFT_0422 0.44 +0.1 402,249 - EX28DRAFT_0422 0.44 +1.6 402,249 - EX28DRAFT_0422 0.44 -0.0 402,271 + EX28DRAFT_0422 0.46 -1.4 402,374 + EX28DRAFT_0422 0.53 -2.0 402,420 + EX28DRAFT_0422 0.56 -0.6 402,517 + EX28DRAFT_0422 0.63 -0.2 402,518 - EX28DRAFT_0422 0.63 +0.2 402,565 + EX28DRAFT_0422 0.67 +0.5 402,566 - EX28DRAFT_0422 0.67 -0.5 402,729 + EX28DRAFT_0422 0.78 +0.6 402,729 + EX28DRAFT_0422 0.78 -0.0 402,730 - EX28DRAFT_0422 0.78 -0.2 402,730 - EX28DRAFT_0422 0.78 -0.2 402,749 - EX28DRAFT_0422 0.80 +0.1 402,980 - -0.1 403,049 + -0.0 403,049 + -0.2 403,053 + -0.4 403,054 - -0.7 403,055 + -0.6 403,055 + +0.4 403,056 - -0.6 403,056 - -0.0 403,056 - -0.6 403,060 - -0.3 403,060 - +1.8 403,060 - -0.4 403,271 - EX28DRAFT_0423 0.24 -0.4 403,271 - EX28DRAFT_0423 0.24 -0.3 403,799 + -1.9 403,852 - +0.1 403,911 - +0.0
Or see this region's nucleotide sequence