Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0196 and EX28DRAFT_0197 are separated by 19 nucleotides EX28DRAFT_0197 and EX28DRAFT_0198 are separated by 289 nucleotides
EX28DRAFT_0196: EX28DRAFT_0196 - transketolase, bacterial and yeast, at 149,310 to 151,298
_0196
EX28DRAFT_0197: EX28DRAFT_0197 - transaldolase, at 151,318 to 152,268
_0197
EX28DRAFT_0198: EX28DRAFT_0198 - Malic enzyme, at 152,558 to 154,837
_0198
Position (kb)
151
152
153 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 150.430 kb on + strand, within EX28DRAFT_0196 at 150.431 kb on - strand, within EX28DRAFT_0196 at 150.431 kb on - strand, within EX28DRAFT_0196 at 150.431 kb on - strand, within EX28DRAFT_0196 at 150.578 kb on + strand, within EX28DRAFT_0196 at 150.679 kb on + strand, within EX28DRAFT_0196 at 150.846 kb on - strand, within EX28DRAFT_0196 at 150.853 kb on + strand, within EX28DRAFT_0196 at 150.853 kb on + strand, within EX28DRAFT_0196 at 150.853 kb on + strand, within EX28DRAFT_0196 at 150.854 kb on - strand, within EX28DRAFT_0196 at 150.854 kb on - strand, within EX28DRAFT_0196 at 150.854 kb on - strand, within EX28DRAFT_0196 at 150.854 kb on - strand, within EX28DRAFT_0196 at 150.857 kb on + strand, within EX28DRAFT_0196 at 150.858 kb on - strand, within EX28DRAFT_0196 at 150.858 kb on - strand, within EX28DRAFT_0196 at 150.858 kb on - strand, within EX28DRAFT_0196 at 150.858 kb on - strand, within EX28DRAFT_0196 at 151.088 kb on - strand, within EX28DRAFT_0196 at 151.281 kb on + strand at 151.298 kb on + strand at 151.298 kb on + strand at 151.298 kb on + strand at 151.298 kb on + strand at 151.298 kb on + strand at 151.299 kb on - strand at 151.299 kb on - strand at 151.299 kb on - strand at 151.299 kb on - strand at 151.516 kb on + strand, within EX28DRAFT_0197 at 151.517 kb on - strand, within EX28DRAFT_0197 at 151.644 kb on - strand, within EX28DRAFT_0197 at 151.644 kb on - strand, within EX28DRAFT_0197 at 151.648 kb on - strand, within EX28DRAFT_0197 at 151.688 kb on + strand, within EX28DRAFT_0197 at 151.688 kb on + strand, within EX28DRAFT_0197 at 151.689 kb on - strand, within EX28DRAFT_0197 at 151.689 kb on - strand, within EX28DRAFT_0197 at 151.724 kb on + strand, within EX28DRAFT_0197 at 151.724 kb on + strand, within EX28DRAFT_0197 at 151.725 kb on - strand, within EX28DRAFT_0197 at 151.725 kb on - strand, within EX28DRAFT_0197 at 151.897 kb on - strand, within EX28DRAFT_0197 at 151.897 kb on - strand, within EX28DRAFT_0197 at 151.897 kb on - strand, within EX28DRAFT_0197 at 151.945 kb on - strand, within EX28DRAFT_0197 at 152.035 kb on - strand, within EX28DRAFT_0197 at 152.099 kb on + strand, within EX28DRAFT_0197 at 152.158 kb on + strand, within EX28DRAFT_0197 at 152.268 kb on + strand at 152.269 kb on - strand at 152.276 kb on + strand at 152.277 kb on - strand at 152.404 kb on - strand at 152.406 kb on - strand at 152.484 kb on + strand at 152.484 kb on + strand at 152.519 kb on - strand at 152.519 kb on - strand at 152.554 kb on + strand at 152.555 kb on - strand at 152.736 kb on - strand at 152.736 kb on - strand at 152.845 kb on - strand, within EX28DRAFT_0198 at 152.990 kb on + strand, within EX28DRAFT_0198 at 152.990 kb on + strand, within EX28DRAFT_0198 at 152.991 kb on - strand, within EX28DRAFT_0198 at 152.991 kb on - strand, within EX28DRAFT_0198 at 153.230 kb on - strand, within EX28DRAFT_0198 at 153.233 kb on + strand, within EX28DRAFT_0198 at 153.233 kb on + strand, within EX28DRAFT_0198 at 153.234 kb on - strand, within EX28DRAFT_0198 at 153.234 kb on - strand, within EX28DRAFT_0198
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 150,430 + EX28DRAFT_0196 0.56 +0.3 150,431 - EX28DRAFT_0196 0.56 +0.4 150,431 - EX28DRAFT_0196 0.56 +0.5 150,431 - EX28DRAFT_0196 0.56 +0.2 150,578 + EX28DRAFT_0196 0.64 -0.3 150,679 + EX28DRAFT_0196 0.69 -0.6 150,846 - EX28DRAFT_0196 0.77 +0.2 150,853 + EX28DRAFT_0196 0.78 +0.1 150,853 + EX28DRAFT_0196 0.78 +0.9 150,853 + EX28DRAFT_0196 0.78 +0.4 150,854 - EX28DRAFT_0196 0.78 -0.9 150,854 - EX28DRAFT_0196 0.78 -0.4 150,854 - EX28DRAFT_0196 0.78 +0.8 150,854 - EX28DRAFT_0196 0.78 -0.5 150,857 + EX28DRAFT_0196 0.78 +0.2 150,858 - EX28DRAFT_0196 0.78 -0.6 150,858 - EX28DRAFT_0196 0.78 -0.1 150,858 - EX28DRAFT_0196 0.78 +1.2 150,858 - EX28DRAFT_0196 0.78 -0.1 151,088 - EX28DRAFT_0196 0.89 -0.2 151,281 + -0.2 151,298 + +0.1 151,298 + +1.0 151,298 + +0.0 151,298 + +0.9 151,298 + -0.3 151,299 - -0.4 151,299 - -1.1 151,299 - -0.4 151,299 - -1.4 151,516 + EX28DRAFT_0197 0.21 +0.1 151,517 - EX28DRAFT_0197 0.21 -0.4 151,644 - EX28DRAFT_0197 0.34 -1.3 151,644 - EX28DRAFT_0197 0.34 +0.1 151,648 - EX28DRAFT_0197 0.35 +0.2 151,688 + EX28DRAFT_0197 0.39 +0.1 151,688 + EX28DRAFT_0197 0.39 +0.2 151,689 - EX28DRAFT_0197 0.39 +0.9 151,689 - EX28DRAFT_0197 0.39 -0.3 151,724 + EX28DRAFT_0197 0.43 +0.1 151,724 + EX28DRAFT_0197 0.43 +0.2 151,725 - EX28DRAFT_0197 0.43 -0.4 151,725 - EX28DRAFT_0197 0.43 -0.4 151,897 - EX28DRAFT_0197 0.61 -1.3 151,897 - EX28DRAFT_0197 0.61 +2.5 151,897 - EX28DRAFT_0197 0.61 -0.3 151,945 - EX28DRAFT_0197 0.66 -0.0 152,035 - EX28DRAFT_0197 0.75 -0.5 152,099 + EX28DRAFT_0197 0.82 +0.0 152,158 + EX28DRAFT_0197 0.88 +0.8 152,268 + -0.3 152,269 - +0.4 152,276 + +0.0 152,277 - +0.6 152,404 - -0.1 152,406 - +0.5 152,484 + -0.8 152,484 + +1.3 152,519 - -2.2 152,519 - -0.8 152,554 + -1.8 152,555 - -0.8 152,736 - -0.2 152,736 - +0.3 152,845 - EX28DRAFT_0198 0.13 -0.3 152,990 + EX28DRAFT_0198 0.19 +0.5 152,990 + EX28DRAFT_0198 0.19 -0.1 152,991 - EX28DRAFT_0198 0.19 +0.0 152,991 - EX28DRAFT_0198 0.19 +0.4 153,230 - EX28DRAFT_0198 0.29 +0.3 153,233 + EX28DRAFT_0198 0.30 +0.2 153,233 + EX28DRAFT_0198 0.30 -1.7 153,234 - EX28DRAFT_0198 0.30 -1.1 153,234 - EX28DRAFT_0198 0.30 -0.7
Or see this region's nucleotide sequence