Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0133 and EX28DRAFT_0134 are separated by 299 nucleotides EX28DRAFT_0134 and EX28DRAFT_0135 are separated by 55 nucleotides EX28DRAFT_0135 and EX28DRAFT_0136 overlap by 4 nucleotides EX28DRAFT_0136 and EX28DRAFT_0137 are separated by 0 nucleotides
EX28DRAFT_0133: EX28DRAFT_0133 - ribosome-associated GTPase EngA, at 76,519 to 77,991
_0133
EX28DRAFT_0134: EX28DRAFT_0134 - amino acid/peptide transporter (Peptide:H+ symporter), bacterial, at 78,291 to 79,835
_0134
EX28DRAFT_0135: EX28DRAFT_0135 - replication restart DNA helicase PriA, at 79,891 to 80,112
_0135
EX28DRAFT_0136: EX28DRAFT_0136 - hypothetical protein, at 80,109 to 80,459
_0136
EX28DRAFT_0137: EX28DRAFT_0137 - Thermolysin metallopeptidase, alpha-helical domain/Thermolysin metallopeptidase, catalytic domain, at 80,460 to 81,479
_0137
Position (kb) 78
79
80 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 77.989 kb on + strand at 78.227 kb on + strand at 78.227 kb on + strand at 78.227 kb on + strand at 78.228 kb on - strand at 78.228 kb on - strand at 78.232 kb on - strand at 78.258 kb on + strand at 78.259 kb on - strand at 78.259 kb on - strand at 78.274 kb on + strand at 78.290 kb on + strand at 78.290 kb on + strand at 78.507 kb on + strand, within EX28DRAFT_0134 at 78.507 kb on + strand, within EX28DRAFT_0134 at 78.508 kb on - strand, within EX28DRAFT_0134 at 78.508 kb on - strand, within EX28DRAFT_0134 at 78.766 kb on - strand, within EX28DRAFT_0134 at 78.766 kb on - strand, within EX28DRAFT_0134 at 78.766 kb on - strand, within EX28DRAFT_0134 at 78.766 kb on - strand, within EX28DRAFT_0134 at 78.767 kb on + strand, within EX28DRAFT_0134 at 78.780 kb on - strand, within EX28DRAFT_0134 at 78.884 kb on + strand, within EX28DRAFT_0134 at 78.922 kb on - strand, within EX28DRAFT_0134 at 79.117 kb on - strand, within EX28DRAFT_0134 at 79.117 kb on - strand, within EX28DRAFT_0134 at 79.125 kb on + strand, within EX28DRAFT_0134 at 79.184 kb on + strand, within EX28DRAFT_0134 at 79.264 kb on - strand, within EX28DRAFT_0134 at 79.355 kb on + strand, within EX28DRAFT_0134 at 79.356 kb on - strand, within EX28DRAFT_0134 at 79.890 kb on + strand at 79.890 kb on + strand at 79.890 kb on + strand at 79.891 kb on - strand at 79.891 kb on - strand at 79.891 kb on - strand at 79.962 kb on + strand, within EX28DRAFT_0135 at 79.963 kb on - strand, within EX28DRAFT_0135 at 79.963 kb on - strand, within EX28DRAFT_0135 at 79.965 kb on + strand, within EX28DRAFT_0135 at 80.009 kb on + strand, within EX28DRAFT_0135 at 80.009 kb on + strand, within EX28DRAFT_0135 at 80.010 kb on - strand, within EX28DRAFT_0135 at 80.018 kb on + strand, within EX28DRAFT_0135 at 80.018 kb on + strand, within EX28DRAFT_0135 at 80.022 kb on - strand, within EX28DRAFT_0135 at 80.070 kb on + strand, within EX28DRAFT_0135 at 80.070 kb on + strand, within EX28DRAFT_0135 at 80.071 kb on - strand, within EX28DRAFT_0135 at 80.076 kb on + strand, within EX28DRAFT_0135 at 80.110 kb on + strand at 80.393 kb on + strand, within EX28DRAFT_0136 at 80.461 kb on + strand at 80.461 kb on + strand at 80.462 kb on - strand at 80.462 kb on - strand at 80.605 kb on + strand, within EX28DRAFT_0137 at 80.662 kb on + strand, within EX28DRAFT_0137
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 77,989 + +0.4 78,227 + +0.9 78,227 + -0.6 78,227 + +0.5 78,228 - -0.8 78,228 - -0.1 78,232 - -1.2 78,258 + -0.5 78,259 - +0.3 78,259 - +0.5 78,274 + -1.8 78,290 + +0.5 78,290 + +1.0 78,507 + EX28DRAFT_0134 0.14 -0.2 78,507 + EX28DRAFT_0134 0.14 +0.3 78,508 - EX28DRAFT_0134 0.14 +0.2 78,508 - EX28DRAFT_0134 0.14 -1.4 78,766 - EX28DRAFT_0134 0.31 +0.1 78,766 - EX28DRAFT_0134 0.31 +0.3 78,766 - EX28DRAFT_0134 0.31 +0.5 78,766 - EX28DRAFT_0134 0.31 +0.6 78,767 + EX28DRAFT_0134 0.31 +0.1 78,780 - EX28DRAFT_0134 0.32 -0.5 78,884 + EX28DRAFT_0134 0.38 +0.2 78,922 - EX28DRAFT_0134 0.41 +0.6 79,117 - EX28DRAFT_0134 0.53 +1.6 79,117 - EX28DRAFT_0134 0.53 +0.8 79,125 + EX28DRAFT_0134 0.54 +0.5 79,184 + EX28DRAFT_0134 0.58 +0.4 79,264 - EX28DRAFT_0134 0.63 -0.1 79,355 + EX28DRAFT_0134 0.69 +0.3 79,356 - EX28DRAFT_0134 0.69 +0.7 79,890 + +0.0 79,890 + -0.2 79,890 + -0.6 79,891 - -0.4 79,891 - +0.7 79,891 - +0.0 79,962 + EX28DRAFT_0135 0.32 +0.4 79,963 - EX28DRAFT_0135 0.32 -0.2 79,963 - EX28DRAFT_0135 0.32 -0.8 79,965 + EX28DRAFT_0135 0.33 -0.3 80,009 + EX28DRAFT_0135 0.53 +1.0 80,009 + EX28DRAFT_0135 0.53 +0.0 80,010 - EX28DRAFT_0135 0.54 +0.1 80,018 + EX28DRAFT_0135 0.57 +0.7 80,018 + EX28DRAFT_0135 0.57 -0.3 80,022 - EX28DRAFT_0135 0.59 -0.5 80,070 + EX28DRAFT_0135 0.81 -0.4 80,070 + EX28DRAFT_0135 0.81 -0.1 80,071 - EX28DRAFT_0135 0.81 -0.3 80,076 + EX28DRAFT_0135 0.83 +0.0 80,110 + +1.1 80,393 + EX28DRAFT_0136 0.81 +0.7 80,461 + -0.6 80,461 + +0.6 80,462 - -1.1 80,462 - +2.8 80,605 + EX28DRAFT_0137 0.14 +0.1 80,662 + EX28DRAFT_0137 0.20 +1.4
Or see this region's nucleotide sequence