Experiment: 150_mag_1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS07965 and AMB_RS07970 are separated by 5 nucleotides AMB_RS07970 and AMB_RS07975 overlap by 4 nucleotides AMB_RS07975 and AMB_RS07980 are separated by 80 nucleotides AMB_RS07980 and AMB_RS07985 are separated by 20 nucleotides AMB_RS07985 and AMB_RS07990 are separated by 148 nucleotides
AMB_RS07965: AMB_RS07965 - SIR2 family protein, at 1,710,070 to 1,710,942
_RS07965
AMB_RS07970: AMB_RS07970 - putative nitrogen fixation protein NifT, at 1,710,948 to 1,711,163
_RS07970
AMB_RS07975: AMB_RS07975 - nitrogen fixation protein NifZ, at 1,711,160 to 1,711,441
_RS07975
AMB_RS07980: AMB_RS07980 - 4Fe-4S ferredoxin, at 1,711,522 to 1,711,710
_RS07980
AMB_RS07985: AMB_RS07985 - nitrogenase cofactor biosynthesis protein NifB, at 1,711,731 to 1,713,227
_RS07985
AMB_RS07990: AMB_RS07990 - Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase GatCAB subunit B, at 1,713,376 to 1,714,833
_RS07990
Position (kb)
1711
1712
1713
1714 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1710.742 kb on - strand, within AMB_RS07965 at 1711.120 kb on + strand, within AMB_RS07970 at 1711.120 kb on + strand, within AMB_RS07970 at 1711.120 kb on + strand, within AMB_RS07970 at 1711.120 kb on + strand, within AMB_RS07970 at 1711.120 kb on + strand, within AMB_RS07970 at 1711.120 kb on + strand, within AMB_RS07970 at 1711.121 kb on - strand, within AMB_RS07970 at 1711.121 kb on - strand, within AMB_RS07970 at 1711.251 kb on + strand, within AMB_RS07975 at 1711.251 kb on + strand, within AMB_RS07975 at 1711.252 kb on - strand, within AMB_RS07975 at 1711.257 kb on + strand, within AMB_RS07975 at 1711.257 kb on + strand, within AMB_RS07975 at 1711.257 kb on + strand, within AMB_RS07975 at 1711.257 kb on + strand, within AMB_RS07975 at 1711.257 kb on + strand, within AMB_RS07975 at 1711.257 kb on + strand, within AMB_RS07975 at 1711.257 kb on + strand, within AMB_RS07975 at 1711.258 kb on - strand, within AMB_RS07975 at 1711.258 kb on - strand, within AMB_RS07975 at 1711.258 kb on - strand, within AMB_RS07975 at 1711.258 kb on - strand, within AMB_RS07975 at 1711.272 kb on + strand, within AMB_RS07975 at 1711.272 kb on + strand, within AMB_RS07975 at 1711.273 kb on - strand, within AMB_RS07975 at 1711.273 kb on - strand, within AMB_RS07975 at 1711.273 kb on - strand, within AMB_RS07975 at 1711.293 kb on + strand, within AMB_RS07975 at 1711.293 kb on + strand, within AMB_RS07975 at 1711.294 kb on - strand, within AMB_RS07975 at 1711.399 kb on - strand, within AMB_RS07975 at 1711.399 kb on - strand, within AMB_RS07975 at 1711.399 kb on - strand, within AMB_RS07975 at 1711.616 kb on + strand, within AMB_RS07980 at 1711.616 kb on + strand, within AMB_RS07980 at 1711.616 kb on + strand, within AMB_RS07980 at 1711.617 kb on - strand, within AMB_RS07980 at 1711.617 kb on - strand, within AMB_RS07980 at 1711.777 kb on + strand at 1711.804 kb on + strand at 1711.804 kb on + strand at 1711.805 kb on - strand at 1711.917 kb on + strand, within AMB_RS07985 at 1711.917 kb on + strand, within AMB_RS07985 at 1711.918 kb on - strand, within AMB_RS07985 at 1711.946 kb on - strand, within AMB_RS07985 at 1711.960 kb on + strand, within AMB_RS07985 at 1711.960 kb on + strand, within AMB_RS07985 at 1711.960 kb on + strand, within AMB_RS07985 at 1711.960 kb on + strand, within AMB_RS07985 at 1712.441 kb on + strand, within AMB_RS07985 at 1712.441 kb on + strand, within AMB_RS07985 at 1712.441 kb on + strand, within AMB_RS07985 at 1712.442 kb on - strand, within AMB_RS07985 at 1712.442 kb on - strand, within AMB_RS07985 at 1712.442 kb on - strand, within AMB_RS07985 at 1712.442 kb on - strand, within AMB_RS07985 at 1712.614 kb on + strand, within AMB_RS07985 at 1712.632 kb on + strand, within AMB_RS07985 at 1712.632 kb on + strand, within AMB_RS07985 at 1712.633 kb on - strand, within AMB_RS07985 at 1712.644 kb on + strand, within AMB_RS07985 at 1712.644 kb on + strand, within AMB_RS07985 at 1712.764 kb on + strand, within AMB_RS07985 at 1712.764 kb on + strand, within AMB_RS07985 at 1712.969 kb on - strand, within AMB_RS07985 at 1712.969 kb on - strand, within AMB_RS07985 at 1712.983 kb on + strand, within AMB_RS07985 at 1712.983 kb on + strand, within AMB_RS07985 at 1712.983 kb on + strand, within AMB_RS07985 at 1712.983 kb on + strand, within AMB_RS07985 at 1713.037 kb on + strand, within AMB_RS07985 at 1713.037 kb on + strand, within AMB_RS07985 at 1713.037 kb on + strand, within AMB_RS07985 at 1713.038 kb on - strand, within AMB_RS07985 at 1713.038 kb on - strand, within AMB_RS07985 at 1713.089 kb on + strand at 1713.089 kb on + strand at 1713.090 kb on - strand at 1713.090 kb on - strand at 1713.251 kb on + strand at 1713.377 kb on + strand at 1713.378 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 150_mag_1 remove 1,710,742 - AMB_RS07965 0.77 -0.4 1,711,120 + AMB_RS07970 0.80 -0.5 1,711,120 + AMB_RS07970 0.80 +2.8 1,711,120 + AMB_RS07970 0.80 -1.0 1,711,120 + AMB_RS07970 0.80 +0.2 1,711,120 + AMB_RS07970 0.80 -0.6 1,711,120 + AMB_RS07970 0.80 -0.1 1,711,121 - AMB_RS07970 0.80 +1.4 1,711,121 - AMB_RS07970 0.80 -0.5 1,711,251 + AMB_RS07975 0.32 -0.9 1,711,251 + AMB_RS07975 0.32 -0.6 1,711,252 - AMB_RS07975 0.33 -0.5 1,711,257 + AMB_RS07975 0.34 -1.3 1,711,257 + AMB_RS07975 0.34 -0.5 1,711,257 + AMB_RS07975 0.34 -0.7 1,711,257 + AMB_RS07975 0.34 -0.5 1,711,257 + AMB_RS07975 0.34 -0.4 1,711,257 + AMB_RS07975 0.34 -1.0 1,711,257 + AMB_RS07975 0.34 +0.6 1,711,258 - AMB_RS07975 0.35 -0.5 1,711,258 - AMB_RS07975 0.35 +0.4 1,711,258 - AMB_RS07975 0.35 -0.7 1,711,258 - AMB_RS07975 0.35 +1.6 1,711,272 + AMB_RS07975 0.40 +0.6 1,711,272 + AMB_RS07975 0.40 +0.3 1,711,273 - AMB_RS07975 0.40 -2.2 1,711,273 - AMB_RS07975 0.40 -0.2 1,711,273 - AMB_RS07975 0.40 -1.7 1,711,293 + AMB_RS07975 0.47 -0.5 1,711,293 + AMB_RS07975 0.47 +0.2 1,711,294 - AMB_RS07975 0.48 -1.2 1,711,399 - AMB_RS07975 0.85 -2.9 1,711,399 - AMB_RS07975 0.85 +0.1 1,711,399 - AMB_RS07975 0.85 +0.6 1,711,616 + AMB_RS07980 0.50 +0.7 1,711,616 + AMB_RS07980 0.50 -1.0 1,711,616 + AMB_RS07980 0.50 -0.2 1,711,617 - AMB_RS07980 0.50 +0.8 1,711,617 - AMB_RS07980 0.50 +1.0 1,711,777 + +1.0 1,711,804 + +0.1 1,711,804 + -0.3 1,711,805 - +0.4 1,711,917 + AMB_RS07985 0.12 -0.5 1,711,917 + AMB_RS07985 0.12 -0.4 1,711,918 - AMB_RS07985 0.12 +1.6 1,711,946 - AMB_RS07985 0.14 -0.5 1,711,960 + AMB_RS07985 0.15 +1.4 1,711,960 + AMB_RS07985 0.15 +1.1 1,711,960 + AMB_RS07985 0.15 -1.4 1,711,960 + AMB_RS07985 0.15 -1.7 1,712,441 + AMB_RS07985 0.47 +0.5 1,712,441 + AMB_RS07985 0.47 -3.5 1,712,441 + AMB_RS07985 0.47 -0.5 1,712,442 - AMB_RS07985 0.47 +0.5 1,712,442 - AMB_RS07985 0.47 -1.9 1,712,442 - AMB_RS07985 0.47 -0.5 1,712,442 - AMB_RS07985 0.47 -0.3 1,712,614 + AMB_RS07985 0.59 -0.5 1,712,632 + AMB_RS07985 0.60 +0.1 1,712,632 + AMB_RS07985 0.60 -1.1 1,712,633 - AMB_RS07985 0.60 -0.0 1,712,644 + AMB_RS07985 0.61 -1.7 1,712,644 + AMB_RS07985 0.61 -0.1 1,712,764 + AMB_RS07985 0.69 -0.4 1,712,764 + AMB_RS07985 0.69 -0.4 1,712,969 - AMB_RS07985 0.83 -0.7 1,712,969 - AMB_RS07985 0.83 -0.2 1,712,983 + AMB_RS07985 0.84 -0.1 1,712,983 + AMB_RS07985 0.84 +0.3 1,712,983 + AMB_RS07985 0.84 -3.0 1,712,983 + AMB_RS07985 0.84 -0.8 1,713,037 + AMB_RS07985 0.87 -0.2 1,713,037 + AMB_RS07985 0.87 +0.5 1,713,037 + AMB_RS07985 0.87 +0.2 1,713,038 - AMB_RS07985 0.87 +0.1 1,713,038 - AMB_RS07985 0.87 -0.1 1,713,089 + +0.4 1,713,089 + -0.4 1,713,090 - +0.6 1,713,090 - +0.6 1,713,251 + -0.4 1,713,377 + -0.3 1,713,378 - -1.3
Or see this region's nucleotide sequence