Strain Fitness in Escherichia coli BW25113 around b1260

Experiment: casaminos (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyciF and yciG are separated by 85 nucleotidesyciG and trpA are separated by 380 nucleotidestrpA and trpB overlap by 1 nucleotides b1258: yciF - hypothetical protein (NCBI), at 1,313,294 to 1,313,794 yciF b1259: yciG - orf, hypothetical protein (VIMSS), at 1,313,880 to 1,314,059 yciG b1260: trpA - tryptophan synthase subunit alpha (NCBI), at 1,314,440 to 1,315,246 trpA b1261: trpB - tryptophan synthase subunit beta (NCBI), at 1,315,246 to 1,316,439 trpB Position (kb) 1314 1315 1316Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 1313.479 kb on - strand, within yciFat 1313.499 kb on - strand, within yciFat 1313.642 kb on + strand, within yciFat 1313.939 kb on - strand, within yciGat 1313.996 kb on - strand, within yciGat 1314.336 kb on + strandat 1314.440 kb on - strandat 1314.440 kb on - strandat 1314.440 kb on - strandat 1314.666 kb on + strand, within trpAat 1314.931 kb on - strand, within trpAat 1315.091 kb on - strand, within trpAat 1315.100 kb on - strand, within trpAat 1315.174 kb on + strandat 1315.218 kb on - strandat 1315.218 kb on - strandat 1315.453 kb on + strand, within trpBat 1315.671 kb on + strand, within trpBat 1315.984 kb on - strand, within trpB

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Per-strain Table

Position Strand Gene LocusTag Fraction casaminos (C)
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1,313,479 - yciF b1258 0.37 +0.3
1,313,499 - yciF b1258 0.41 -0.5
1,313,642 + yciF b1258 0.69 -0.7
1,313,939 - yciG b1259 0.33 +0.2
1,313,996 - yciG b1259 0.64 +0.6
1,314,336 + -1.1
1,314,440 - -0.1
1,314,440 - +0.8
1,314,440 - -1.1
1,314,666 + trpA b1260 0.28 -3.5
1,314,931 - trpA b1260 0.61 -2.2
1,315,091 - trpA b1260 0.81 -3.9
1,315,100 - trpA b1260 0.82 -3.6
1,315,174 + -4.2
1,315,218 - -2.0
1,315,218 - -4.3
1,315,453 + trpB b1261 0.17 -3.3
1,315,671 + trpB b1261 0.36 -4.6
1,315,984 - trpB b1261 0.62 -3.5

Or see this region's nucleotide sequence