Strain Fitness in Enterobacter asburiae PDN3 around EX28DRAFT_4371

Experiment: M9 with casaminos and trichloroethylene 76.3 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntEX28DRAFT_4369 and EX28DRAFT_4370 are separated by 44 nucleotidesEX28DRAFT_4370 and EX28DRAFT_4371 are separated by 8 nucleotidesEX28DRAFT_4371 and EX28DRAFT_4372 are separated by 199 nucleotidesEX28DRAFT_4372 and EX28DRAFT_4373 are separated by 2 nucleotidesEX28DRAFT_4373 and EX28DRAFT_4374 are separated by 39 nucleotides EX28DRAFT_4369: EX28DRAFT_4369 - D-tyrosyl-tRNA(Tyr) deacylase, at 35,480 to 35,917 _4369 EX28DRAFT_4370: EX28DRAFT_4370 - thioesterase domain, putative, at 35,962 to 36,903 _4370 EX28DRAFT_4371: EX28DRAFT_4371 - Esterase/lipase, at 36,912 to 37,832 _4371 EX28DRAFT_4372: EX28DRAFT_4372 - Ribbon-helix-helix protein, copG family, at 38,032 to 38,274 _4372 EX28DRAFT_4373: EX28DRAFT_4373 - Predicted nucleic acid-binding protein, contains PIN domain, at 38,277 to 38,648 _4373 EX28DRAFT_4374: EX28DRAFT_4374 - formate dehydrogenase accessory protein FdhE, at 38,688 to 39,617 _4374 Position (kb) 36 37 38Strain fitness (log2 ratio) -2 -1 0 1at 35.956 kb on - strandat 35.956 kb on - strandat 35.956 kb on - strandat 35.961 kb on + strandat 35.961 kb on + strandat 35.962 kb on - strandat 35.962 kb on - strandat 35.962 kb on - strandat 35.965 kb on + strandat 35.965 kb on + strandat 35.965 kb on + strandat 35.966 kb on - strandat 35.966 kb on - strandat 36.010 kb on + strandat 36.010 kb on + strandat 36.011 kb on - strandat 36.145 kb on + strand, within EX28DRAFT_4370at 36.145 kb on + strand, within EX28DRAFT_4370at 36.146 kb on - strand, within EX28DRAFT_4370at 36.146 kb on - strand, within EX28DRAFT_4370at 36.146 kb on - strand, within EX28DRAFT_4370at 36.225 kb on - strand, within EX28DRAFT_4370at 36.411 kb on + strand, within EX28DRAFT_4370at 36.411 kb on + strand, within EX28DRAFT_4370at 36.412 kb on - strand, within EX28DRAFT_4370at 36.505 kb on + strand, within EX28DRAFT_4370at 36.506 kb on - strand, within EX28DRAFT_4370at 36.506 kb on - strand, within EX28DRAFT_4370at 36.522 kb on + strand, within EX28DRAFT_4370at 36.687 kb on + strand, within EX28DRAFT_4370at 36.783 kb on - strand, within EX28DRAFT_4370at 36.842 kb on - strandat 36.842 kb on - strandat 36.842 kb on - strandat 36.842 kb on - strandat 36.842 kb on - strandat 36.842 kb on - strandat 36.842 kb on - strandat 36.901 kb on + strandat 36.932 kb on + strandat 36.933 kb on - strandat 36.933 kb on - strandat 37.063 kb on + strand, within EX28DRAFT_4371at 37.064 kb on - strand, within EX28DRAFT_4371at 37.064 kb on - strand, within EX28DRAFT_4371at 37.097 kb on - strand, within EX28DRAFT_4371at 37.230 kb on - strand, within EX28DRAFT_4371at 37.232 kb on + strand, within EX28DRAFT_4371at 37.232 kb on + strand, within EX28DRAFT_4371at 37.232 kb on + strand, within EX28DRAFT_4371at 37.232 kb on + strand, within EX28DRAFT_4371at 37.232 kb on + strand, within EX28DRAFT_4371at 37.232 kb on + strand, within EX28DRAFT_4371at 37.232 kb on + strand, within EX28DRAFT_4371at 37.232 kb on + strand, within EX28DRAFT_4371at 37.232 kb on + strand, within EX28DRAFT_4371at 37.233 kb on - strand, within EX28DRAFT_4371at 37.233 kb on - strand, within EX28DRAFT_4371at 37.233 kb on - strand, within EX28DRAFT_4371at 37.233 kb on - strand, within EX28DRAFT_4371at 37.235 kb on - strand, within EX28DRAFT_4371at 37.248 kb on - strand, within EX28DRAFT_4371at 37.255 kb on + strand, within EX28DRAFT_4371at 37.255 kb on + strand, within EX28DRAFT_4371at 37.291 kb on + strand, within EX28DRAFT_4371at 37.294 kb on - strand, within EX28DRAFT_4371at 37.295 kb on + strand, within EX28DRAFT_4371at 37.296 kb on - strand, within EX28DRAFT_4371at 37.296 kb on - strand, within EX28DRAFT_4371at 37.296 kb on - strand, within EX28DRAFT_4371at 37.296 kb on - strand, within EX28DRAFT_4371at 37.296 kb on - strand, within EX28DRAFT_4371at 37.301 kb on + strand, within EX28DRAFT_4371at 37.301 kb on + strand, within EX28DRAFT_4371at 37.302 kb on - strand, within EX28DRAFT_4371at 37.308 kb on + strand, within EX28DRAFT_4371at 37.309 kb on - strand, within EX28DRAFT_4371at 37.403 kb on + strand, within EX28DRAFT_4371at 37.404 kb on - strand, within EX28DRAFT_4371at 37.404 kb on - strand, within EX28DRAFT_4371at 37.404 kb on - strand, within EX28DRAFT_4371at 37.481 kb on + strand, within EX28DRAFT_4371at 37.482 kb on - strand, within EX28DRAFT_4371at 37.482 kb on - strand, within EX28DRAFT_4371at 37.482 kb on - strand, within EX28DRAFT_4371at 37.482 kb on - strand, within EX28DRAFT_4371at 37.532 kb on - strand, within EX28DRAFT_4371at 37.583 kb on + strand, within EX28DRAFT_4371at 37.600 kb on + strand, within EX28DRAFT_4371at 37.603 kb on + strand, within EX28DRAFT_4371at 37.603 kb on + strand, within EX28DRAFT_4371at 37.604 kb on - strand, within EX28DRAFT_4371at 37.604 kb on - strand, within EX28DRAFT_4371at 37.634 kb on + strand, within EX28DRAFT_4371at 37.635 kb on - strand, within EX28DRAFT_4371at 37.635 kb on - strand, within EX28DRAFT_4371at 37.732 kb on + strand, within EX28DRAFT_4371at 37.732 kb on + strand, within EX28DRAFT_4371at 37.732 kb on + strand, within EX28DRAFT_4371at 37.733 kb on - strand, within EX28DRAFT_4371at 37.859 kb on + strandat 37.860 kb on - strandat 37.879 kb on + strandat 37.880 kb on - strandat 37.953 kb on + strandat 37.953 kb on + strandat 37.987 kb on + strandat 37.987 kb on + strandat 37.988 kb on - strandat 38.019 kb on - strandat 38.019 kb on - strandat 38.022 kb on - strandat 38.031 kb on + strandat 38.031 kb on + strandat 38.032 kb on - strandat 38.032 kb on - strandat 38.090 kb on + strand, within EX28DRAFT_4372at 38.091 kb on - strand, within EX28DRAFT_4372at 38.167 kb on + strand, within EX28DRAFT_4372at 38.168 kb on - strand, within EX28DRAFT_4372at 38.246 kb on - strand, within EX28DRAFT_4372at 38.272 kb on + strandat 38.273 kb on - strandat 38.307 kb on - strandat 38.307 kb on - strandat 38.307 kb on - strandat 38.307 kb on - strandat 38.325 kb on - strand, within EX28DRAFT_4373at 38.597 kb on + strand, within EX28DRAFT_4373at 38.646 kb on + strandat 38.685 kb on + strandat 38.685 kb on + strandat 38.685 kb on + strandat 38.689 kb on + strandat 38.689 kb on + strandat 38.689 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM
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35,956 - +0.1
35,956 - -0.7
35,956 - -0.3
35,961 + +0.4
35,961 + +0.5
35,962 - +0.3
35,962 - +0.2
35,962 - -0.1
35,965 + +0.3
35,965 + +0.3
35,965 + +0.4
35,966 - -0.1
35,966 - -0.4
36,010 + +0.6
36,010 + -0.2
36,011 - -0.9
36,145 + EX28DRAFT_4370 0.19 +0.6
36,145 + EX28DRAFT_4370 0.19 +0.6
36,146 - EX28DRAFT_4370 0.20 -0.6
36,146 - EX28DRAFT_4370 0.20 +1.1
36,146 - EX28DRAFT_4370 0.20 +0.4
36,225 - EX28DRAFT_4370 0.28 +0.3
36,411 + EX28DRAFT_4370 0.48 +0.0
36,411 + EX28DRAFT_4370 0.48 +0.3
36,412 - EX28DRAFT_4370 0.48 +1.3
36,505 + EX28DRAFT_4370 0.58 -1.3
36,506 - EX28DRAFT_4370 0.58 +0.1
36,506 - EX28DRAFT_4370 0.58 -0.2
36,522 + EX28DRAFT_4370 0.59 -0.8
36,687 + EX28DRAFT_4370 0.77 +0.5
36,783 - EX28DRAFT_4370 0.87 -1.1
36,842 - -2.1
36,842 - +1.1
36,842 - -0.2
36,842 - -1.9
36,842 - -0.3
36,842 - -1.4
36,842 - -0.1
36,901 + +0.2
36,932 + -0.2
36,933 - +0.1
36,933 - -0.6
37,063 + EX28DRAFT_4371 0.16 +0.3
37,064 - EX28DRAFT_4371 0.17 +1.1
37,064 - EX28DRAFT_4371 0.17 +0.9
37,097 - EX28DRAFT_4371 0.20 -0.7
37,230 - EX28DRAFT_4371 0.35 -0.6
37,232 + EX28DRAFT_4371 0.35 -0.3
37,232 + EX28DRAFT_4371 0.35 -0.4
37,232 + EX28DRAFT_4371 0.35 -0.1
37,232 + EX28DRAFT_4371 0.35 -1.1
37,232 + EX28DRAFT_4371 0.35 +0.1
37,232 + EX28DRAFT_4371 0.35 +0.4
37,232 + EX28DRAFT_4371 0.35 +0.8
37,232 + EX28DRAFT_4371 0.35 +1.0
37,232 + EX28DRAFT_4371 0.35 +1.0
37,233 - EX28DRAFT_4371 0.35 +0.0
37,233 - EX28DRAFT_4371 0.35 +0.9
37,233 - EX28DRAFT_4371 0.35 +0.5
37,233 - EX28DRAFT_4371 0.35 +0.6
37,235 - EX28DRAFT_4371 0.35 +0.1
37,248 - EX28DRAFT_4371 0.36 +0.0
37,255 + EX28DRAFT_4371 0.37 -1.5
37,255 + EX28DRAFT_4371 0.37 +0.8
37,291 + EX28DRAFT_4371 0.41 +0.2
37,294 - EX28DRAFT_4371 0.41 -1.7
37,295 + EX28DRAFT_4371 0.42 -0.1
37,296 - EX28DRAFT_4371 0.42 +0.4
37,296 - EX28DRAFT_4371 0.42 -0.1
37,296 - EX28DRAFT_4371 0.42 +0.1
37,296 - EX28DRAFT_4371 0.42 +0.0
37,296 - EX28DRAFT_4371 0.42 +0.4
37,301 + EX28DRAFT_4371 0.42 +0.1
37,301 + EX28DRAFT_4371 0.42 +0.3
37,302 - EX28DRAFT_4371 0.42 -0.1
37,308 + EX28DRAFT_4371 0.43 +0.1
37,309 - EX28DRAFT_4371 0.43 -0.2
37,403 + EX28DRAFT_4371 0.53 +0.6
37,404 - EX28DRAFT_4371 0.53 -0.1
37,404 - EX28DRAFT_4371 0.53 -0.0
37,404 - EX28DRAFT_4371 0.53 +0.2
37,481 + EX28DRAFT_4371 0.62 -0.0
37,482 - EX28DRAFT_4371 0.62 +0.1
37,482 - EX28DRAFT_4371 0.62 -0.4
37,482 - EX28DRAFT_4371 0.62 -0.4
37,482 - EX28DRAFT_4371 0.62 -0.2
37,532 - EX28DRAFT_4371 0.67 -0.3
37,583 + EX28DRAFT_4371 0.73 +0.3
37,600 + EX28DRAFT_4371 0.75 +0.2
37,603 + EX28DRAFT_4371 0.75 -0.1
37,603 + EX28DRAFT_4371 0.75 -0.1
37,604 - EX28DRAFT_4371 0.75 +0.4
37,604 - EX28DRAFT_4371 0.75 -0.1
37,634 + EX28DRAFT_4371 0.78 +0.9
37,635 - EX28DRAFT_4371 0.79 -1.7
37,635 - EX28DRAFT_4371 0.79 -0.3
37,732 + EX28DRAFT_4371 0.89 -0.1
37,732 + EX28DRAFT_4371 0.89 +1.6
37,732 + EX28DRAFT_4371 0.89 -0.7
37,733 - EX28DRAFT_4371 0.89 +0.4
37,859 + -0.9
37,860 - -0.4
37,879 + +0.4
37,880 - +0.8
37,953 + -1.9
37,953 + +0.1
37,987 + -0.2
37,987 + +0.1
37,988 - -0.2
38,019 - -1.9
38,019 - -0.3
38,022 - -1.3
38,031 + +0.6
38,031 + +0.4
38,032 - -1.5
38,032 - -0.2
38,090 + EX28DRAFT_4372 0.24 -0.5
38,091 - EX28DRAFT_4372 0.24 +0.3
38,167 + EX28DRAFT_4372 0.56 -0.3
38,168 - EX28DRAFT_4372 0.56 -0.3
38,246 - EX28DRAFT_4372 0.88 -0.7
38,272 + +1.1
38,273 - +0.6
38,307 - +0.7
38,307 - -0.1
38,307 - +1.4
38,307 - +0.5
38,325 - EX28DRAFT_4373 0.13 -0.3
38,597 + EX28DRAFT_4373 0.86 -0.1
38,646 + -0.3
38,685 + +0.9
38,685 + +0.1
38,685 + +1.5
38,689 + +0.9
38,689 + +0.4
38,689 + +0.4

Or see this region's nucleotide sequence