Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_4261 and EX28DRAFT_4262 are separated by 184 nucleotides EX28DRAFT_4262 and EX28DRAFT_4263 are separated by 136 nucleotides EX28DRAFT_4263 and EX28DRAFT_4264 are separated by 82 nucleotides
EX28DRAFT_4261: EX28DRAFT_4261 - NADPH:quinone reductase and related Zn-dependent oxidoreductases, at 66,324 to 67,307
_4261
EX28DRAFT_4262: EX28DRAFT_4262 - phage shock protein G, at 67,492 to 67,734
_4262
EX28DRAFT_4263: EX28DRAFT_4263 - tRNA dihydrouridine synthase A, at 67,871 to 68,865
_4263
EX28DRAFT_4264: EX28DRAFT_4264 - F plasmid transfer operon, TraF, protein, at 68,948 to 70,258
_4264
Position (kb)
67
68
69 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 66.893 kb on + strand, within EX28DRAFT_4261 at 66.893 kb on + strand, within EX28DRAFT_4261 at 66.894 kb on - strand, within EX28DRAFT_4261 at 66.894 kb on - strand, within EX28DRAFT_4261 at 66.897 kb on + strand, within EX28DRAFT_4261 at 66.963 kb on + strand, within EX28DRAFT_4261 at 66.963 kb on + strand, within EX28DRAFT_4261 at 67.077 kb on - strand, within EX28DRAFT_4261 at 67.101 kb on + strand, within EX28DRAFT_4261 at 67.101 kb on + strand, within EX28DRAFT_4261 at 67.102 kb on - strand, within EX28DRAFT_4261 at 67.102 kb on - strand, within EX28DRAFT_4261 at 67.128 kb on + strand, within EX28DRAFT_4261 at 67.210 kb on + strand at 67.211 kb on - strand at 67.211 kb on - strand at 67.365 kb on + strand at 67.365 kb on + strand at 67.366 kb on - strand at 67.369 kb on + strand at 67.369 kb on + strand at 67.374 kb on + strand at 67.406 kb on - strand at 67.420 kb on + strand at 67.424 kb on + strand at 67.425 kb on - strand at 67.464 kb on - strand at 67.470 kb on - strand at 67.489 kb on - strand at 67.489 kb on - strand at 67.583 kb on - strand, within EX28DRAFT_4262 at 67.768 kb on - strand at 68.002 kb on + strand, within EX28DRAFT_4263 at 68.025 kb on + strand, within EX28DRAFT_4263 at 68.025 kb on + strand, within EX28DRAFT_4263 at 68.025 kb on + strand, within EX28DRAFT_4263 at 68.055 kb on + strand, within EX28DRAFT_4263 at 68.055 kb on + strand, within EX28DRAFT_4263 at 68.061 kb on + strand, within EX28DRAFT_4263 at 68.062 kb on - strand, within EX28DRAFT_4263 at 68.155 kb on - strand, within EX28DRAFT_4263 at 68.185 kb on + strand, within EX28DRAFT_4263 at 68.186 kb on - strand, within EX28DRAFT_4263 at 68.689 kb on - strand, within EX28DRAFT_4263 at 68.689 kb on - strand, within EX28DRAFT_4263 at 68.698 kb on - strand, within EX28DRAFT_4263 at 68.745 kb on - strand, within EX28DRAFT_4263 at 68.755 kb on - strand, within EX28DRAFT_4263 at 68.781 kb on - strand at 68.852 kb on - strand at 68.876 kb on + strand at 68.904 kb on + strand at 68.904 kb on + strand at 68.905 kb on - strand at 68.905 kb on - strand at 68.905 kb on - strand at 68.906 kb on + strand at 68.949 kb on + strand at 68.950 kb on - strand at 69.000 kb on + strand at 69.107 kb on + strand, within EX28DRAFT_4264 at 69.131 kb on - strand, within EX28DRAFT_4264 at 69.163 kb on + strand, within EX28DRAFT_4264 at 69.163 kb on + strand, within EX28DRAFT_4264 at 69.163 kb on + strand, within EX28DRAFT_4264 at 69.164 kb on - strand, within EX28DRAFT_4264 at 69.271 kb on - strand, within EX28DRAFT_4264 at 69.281 kb on - strand, within EX28DRAFT_4264 at 69.282 kb on + strand, within EX28DRAFT_4264 at 69.283 kb on - strand, within EX28DRAFT_4264 at 69.283 kb on - strand, within EX28DRAFT_4264 at 69.477 kb on + strand, within EX28DRAFT_4264 at 69.492 kb on + strand, within EX28DRAFT_4264 at 69.493 kb on - strand, within EX28DRAFT_4264 at 69.493 kb on - strand, within EX28DRAFT_4264 at 69.496 kb on + strand, within EX28DRAFT_4264 at 69.496 kb on + strand, within EX28DRAFT_4264 at 69.497 kb on - strand, within EX28DRAFT_4264 at 69.498 kb on + strand, within EX28DRAFT_4264 at 69.498 kb on + strand, within EX28DRAFT_4264 at 69.498 kb on + strand, within EX28DRAFT_4264 at 69.498 kb on + strand, within EX28DRAFT_4264 at 69.499 kb on - strand, within EX28DRAFT_4264 at 69.499 kb on - strand, within EX28DRAFT_4264 at 69.499 kb on - strand, within EX28DRAFT_4264 at 69.499 kb on - strand, within EX28DRAFT_4264 at 69.499 kb on - strand, within EX28DRAFT_4264 at 69.499 kb on - strand, within EX28DRAFT_4264 at 69.717 kb on + strand, within EX28DRAFT_4264 at 69.718 kb on - strand, within EX28DRAFT_4264
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 66,893 + EX28DRAFT_4261 0.58 +0.3 66,893 + EX28DRAFT_4261 0.58 +0.4 66,894 - EX28DRAFT_4261 0.58 +0.1 66,894 - EX28DRAFT_4261 0.58 -0.1 66,897 + EX28DRAFT_4261 0.58 -0.0 66,963 + EX28DRAFT_4261 0.65 -0.5 66,963 + EX28DRAFT_4261 0.65 -0.8 67,077 - EX28DRAFT_4261 0.77 -0.5 67,101 + EX28DRAFT_4261 0.79 +0.4 67,101 + EX28DRAFT_4261 0.79 +0.7 67,102 - EX28DRAFT_4261 0.79 +0.0 67,102 - EX28DRAFT_4261 0.79 +0.1 67,128 + EX28DRAFT_4261 0.82 -0.8 67,210 + +0.9 67,211 - -0.8 67,211 - -0.1 67,365 + -1.2 67,365 + -0.2 67,366 - +0.8 67,369 + +0.2 67,369 + +0.2 67,374 + -0.6 67,406 - +0.3 67,420 + -0.3 67,424 + -0.6 67,425 - -0.1 67,464 - +0.0 67,470 - +0.8 67,489 - +0.6 67,489 - +0.8 67,583 - EX28DRAFT_4262 0.37 -0.6 67,768 - +0.5 68,002 + EX28DRAFT_4263 0.13 +2.4 68,025 + EX28DRAFT_4263 0.15 +1.3 68,025 + EX28DRAFT_4263 0.15 -0.8 68,025 + EX28DRAFT_4263 0.15 +0.1 68,055 + EX28DRAFT_4263 0.18 -0.5 68,055 + EX28DRAFT_4263 0.18 -0.3 68,061 + EX28DRAFT_4263 0.19 -0.7 68,062 - EX28DRAFT_4263 0.19 -0.1 68,155 - EX28DRAFT_4263 0.29 -1.8 68,185 + EX28DRAFT_4263 0.32 -0.3 68,186 - EX28DRAFT_4263 0.32 +0.1 68,689 - EX28DRAFT_4263 0.82 +0.8 68,689 - EX28DRAFT_4263 0.82 -3.1 68,698 - EX28DRAFT_4263 0.83 -0.3 68,745 - EX28DRAFT_4263 0.88 -0.4 68,755 - EX28DRAFT_4263 0.89 -0.6 68,781 - -0.4 68,852 - +0.4 68,876 + +0.3 68,904 + +0.8 68,904 + +0.8 68,905 - -0.1 68,905 - +0.4 68,905 - -0.0 68,906 + -0.1 68,949 + +0.2 68,950 - -0.1 69,000 + -0.0 69,107 + EX28DRAFT_4264 0.12 +0.7 69,131 - EX28DRAFT_4264 0.14 -0.3 69,163 + EX28DRAFT_4264 0.16 +0.0 69,163 + EX28DRAFT_4264 0.16 +0.8 69,163 + EX28DRAFT_4264 0.16 -1.0 69,164 - EX28DRAFT_4264 0.16 +2.4 69,271 - EX28DRAFT_4264 0.25 +0.8 69,281 - EX28DRAFT_4264 0.25 -1.0 69,282 + EX28DRAFT_4264 0.25 -1.1 69,283 - EX28DRAFT_4264 0.26 -0.2 69,283 - EX28DRAFT_4264 0.26 +0.3 69,477 + EX28DRAFT_4264 0.40 +0.2 69,492 + EX28DRAFT_4264 0.41 +0.6 69,493 - EX28DRAFT_4264 0.42 -0.7 69,493 - EX28DRAFT_4264 0.42 -1.4 69,496 + EX28DRAFT_4264 0.42 +1.2 69,496 + EX28DRAFT_4264 0.42 -0.4 69,497 - EX28DRAFT_4264 0.42 -0.7 69,498 + EX28DRAFT_4264 0.42 -0.4 69,498 + EX28DRAFT_4264 0.42 -1.8 69,498 + EX28DRAFT_4264 0.42 -0.1 69,498 + EX28DRAFT_4264 0.42 -0.7 69,499 - EX28DRAFT_4264 0.42 -0.1 69,499 - EX28DRAFT_4264 0.42 -1.2 69,499 - EX28DRAFT_4264 0.42 +0.4 69,499 - EX28DRAFT_4264 0.42 -0.6 69,499 - EX28DRAFT_4264 0.42 -0.1 69,499 - EX28DRAFT_4264 0.42 +0.7 69,717 + EX28DRAFT_4264 0.59 -1.5 69,718 - EX28DRAFT_4264 0.59 -1.8
Or see this region's nucleotide sequence