Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_4108 and EX28DRAFT_4109 are separated by 145 nucleotides EX28DRAFT_4109 and EX28DRAFT_4110 are separated by 4 nucleotides EX28DRAFT_4110 and EX28DRAFT_4111 are separated by 72 nucleotides
EX28DRAFT_4108: EX28DRAFT_4108 - Predicted permease, at 79,800 to 80,849
_4108
EX28DRAFT_4109: EX28DRAFT_4109 - Arabinose efflux permease, at 80,995 to 82,212
_4109
EX28DRAFT_4110: EX28DRAFT_4110 - hypothetical protein, at 82,217 to 82,774
_4110
EX28DRAFT_4111: EX28DRAFT_4111 - conserved hypothetical integral membrane protein, at 82,847 to 83,512
_4111
Position (kb) 80
81
82
83 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 80.033 kb on + strand, within EX28DRAFT_4108 at 80.034 kb on - strand, within EX28DRAFT_4108 at 80.034 kb on - strand, within EX28DRAFT_4108 at 80.034 kb on - strand, within EX28DRAFT_4108 at 80.074 kb on + strand, within EX28DRAFT_4108 at 80.075 kb on - strand, within EX28DRAFT_4108 at 80.084 kb on + strand, within EX28DRAFT_4108 at 80.146 kb on + strand, within EX28DRAFT_4108 at 80.147 kb on - strand, within EX28DRAFT_4108 at 80.200 kb on - strand, within EX28DRAFT_4108 at 80.200 kb on - strand, within EX28DRAFT_4108 at 80.263 kb on + strand, within EX28DRAFT_4108 at 80.264 kb on - strand, within EX28DRAFT_4108 at 80.264 kb on - strand, within EX28DRAFT_4108 at 80.341 kb on + strand, within EX28DRAFT_4108 at 80.341 kb on + strand, within EX28DRAFT_4108 at 80.342 kb on - strand, within EX28DRAFT_4108 at 80.370 kb on - strand, within EX28DRAFT_4108 at 80.370 kb on - strand, within EX28DRAFT_4108 at 80.370 kb on - strand, within EX28DRAFT_4108 at 80.443 kb on + strand, within EX28DRAFT_4108 at 80.496 kb on - strand, within EX28DRAFT_4108 at 80.496 kb on - strand, within EX28DRAFT_4108 at 80.504 kb on + strand, within EX28DRAFT_4108 at 80.560 kb on + strand, within EX28DRAFT_4108 at 80.560 kb on + strand, within EX28DRAFT_4108 at 80.708 kb on + strand, within EX28DRAFT_4108 at 80.708 kb on + strand, within EX28DRAFT_4108 at 80.750 kb on + strand at 80.750 kb on + strand at 80.750 kb on + strand at 80.860 kb on + strand at 80.863 kb on + strand at 80.864 kb on - strand at 80.864 kb on - strand at 80.877 kb on - strand at 80.882 kb on + strand at 80.883 kb on - strand at 80.883 kb on - strand at 80.883 kb on - strand at 80.941 kb on + strand at 80.957 kb on + strand at 81.082 kb on + strand at 81.083 kb on - strand at 81.083 kb on - strand at 81.121 kb on + strand, within EX28DRAFT_4109 at 81.121 kb on + strand, within EX28DRAFT_4109 at 81.170 kb on + strand, within EX28DRAFT_4109 at 81.182 kb on - strand, within EX28DRAFT_4109 at 81.293 kb on - strand, within EX28DRAFT_4109 at 81.293 kb on - strand, within EX28DRAFT_4109 at 81.473 kb on - strand, within EX28DRAFT_4109 at 81.727 kb on + strand, within EX28DRAFT_4109 at 81.774 kb on + strand, within EX28DRAFT_4109 at 81.870 kb on - strand, within EX28DRAFT_4109 at 81.983 kb on + strand, within EX28DRAFT_4109 at 82.027 kb on + strand, within EX28DRAFT_4109 at 82.027 kb on + strand, within EX28DRAFT_4109 at 82.028 kb on - strand, within EX28DRAFT_4109 at 82.044 kb on + strand, within EX28DRAFT_4109 at 82.111 kb on + strand at 82.111 kb on + strand at 82.111 kb on + strand at 82.111 kb on + strand at 82.112 kb on - strand at 82.237 kb on + strand at 82.238 kb on - strand at 82.418 kb on - strand, within EX28DRAFT_4110 at 82.712 kb on - strand, within EX28DRAFT_4110 at 82.756 kb on - strand at 82.756 kb on - strand at 82.760 kb on - strand at 82.880 kb on - strand at 82.904 kb on - strand at 82.965 kb on + strand, within EX28DRAFT_4111 at 83.050 kb on + strand, within EX28DRAFT_4111 at 83.165 kb on - strand, within EX28DRAFT_4111
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 80,033 + EX28DRAFT_4108 0.22 +2.0 80,034 - EX28DRAFT_4108 0.22 +0.1 80,034 - EX28DRAFT_4108 0.22 +0.3 80,034 - EX28DRAFT_4108 0.22 -0.2 80,074 + EX28DRAFT_4108 0.26 +1.0 80,075 - EX28DRAFT_4108 0.26 -0.2 80,084 + EX28DRAFT_4108 0.27 +1.3 80,146 + EX28DRAFT_4108 0.33 -0.2 80,147 - EX28DRAFT_4108 0.33 +1.2 80,200 - EX28DRAFT_4108 0.38 +0.3 80,200 - EX28DRAFT_4108 0.38 -0.5 80,263 + EX28DRAFT_4108 0.44 +0.6 80,264 - EX28DRAFT_4108 0.44 -0.5 80,264 - EX28DRAFT_4108 0.44 -0.2 80,341 + EX28DRAFT_4108 0.52 -2.9 80,341 + EX28DRAFT_4108 0.52 -0.1 80,342 - EX28DRAFT_4108 0.52 +0.1 80,370 - EX28DRAFT_4108 0.54 -0.7 80,370 - EX28DRAFT_4108 0.54 +0.5 80,370 - EX28DRAFT_4108 0.54 +0.4 80,443 + EX28DRAFT_4108 0.61 -2.8 80,496 - EX28DRAFT_4108 0.66 +0.1 80,496 - EX28DRAFT_4108 0.66 -0.2 80,504 + EX28DRAFT_4108 0.67 +0.3 80,560 + EX28DRAFT_4108 0.72 +0.1 80,560 + EX28DRAFT_4108 0.72 +0.2 80,708 + EX28DRAFT_4108 0.86 -1.4 80,708 + EX28DRAFT_4108 0.86 +0.1 80,750 + +0.0 80,750 + -0.3 80,750 + -0.2 80,860 + +0.4 80,863 + -0.3 80,864 - +0.1 80,864 - +0.1 80,877 - -0.0 80,882 + +0.3 80,883 - -0.4 80,883 - +0.0 80,883 - +1.3 80,941 + -0.0 80,957 + -0.2 81,082 + -1.7 81,083 - -0.2 81,083 - +0.8 81,121 + EX28DRAFT_4109 0.10 +0.1 81,121 + EX28DRAFT_4109 0.10 +1.2 81,170 + EX28DRAFT_4109 0.14 +0.6 81,182 - EX28DRAFT_4109 0.15 -0.8 81,293 - EX28DRAFT_4109 0.24 +0.3 81,293 - EX28DRAFT_4109 0.24 -0.8 81,473 - EX28DRAFT_4109 0.39 -0.2 81,727 + EX28DRAFT_4109 0.60 +0.1 81,774 + EX28DRAFT_4109 0.64 +0.2 81,870 - EX28DRAFT_4109 0.72 -0.6 81,983 + EX28DRAFT_4109 0.81 -0.4 82,027 + EX28DRAFT_4109 0.85 -1.2 82,027 + EX28DRAFT_4109 0.85 +1.3 82,028 - EX28DRAFT_4109 0.85 -1.4 82,044 + EX28DRAFT_4109 0.86 -0.4 82,111 + +0.1 82,111 + +0.4 82,111 + -0.5 82,111 + +0.5 82,112 - -0.9 82,237 + +2.6 82,238 - -0.2 82,418 - EX28DRAFT_4110 0.36 -0.6 82,712 - EX28DRAFT_4110 0.89 -0.9 82,756 - -0.7 82,756 - +0.5 82,760 - -0.1 82,880 - +0.4 82,904 - -0.1 82,965 + EX28DRAFT_4111 0.18 +0.6 83,050 + EX28DRAFT_4111 0.30 -0.7 83,165 - EX28DRAFT_4111 0.48 +1.2
Or see this region's nucleotide sequence