Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3964 and EX28DRAFT_3965 are separated by 204 nucleotides EX28DRAFT_3965 and EX28DRAFT_3966 are separated by 17 nucleotides
EX28DRAFT_3964: EX28DRAFT_3964 - D-serine deaminase transcriptional activator, at 172,067 to 172,993
_3964
EX28DRAFT_3965: EX28DRAFT_3965 - gluconate transporter, at 173,198 to 174,535
_3965
EX28DRAFT_3966: EX28DRAFT_3966 - D-serine ammonia-lyase (EC 4.3.1.18), at 174,553 to 175,839
_3966
Position (kb) 173
174
175 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 172.295 kb on - strand, within EX28DRAFT_3964 at 172.450 kb on - strand, within EX28DRAFT_3964 at 172.519 kb on + strand, within EX28DRAFT_3964 at 172.932 kb on + strand at 173.027 kb on + strand at 173.129 kb on + strand at 173.181 kb on + strand at 173.181 kb on + strand at 173.181 kb on + strand at 173.182 kb on - strand at 173.182 kb on - strand at 173.197 kb on + strand at 173.510 kb on - strand, within EX28DRAFT_3965 at 173.704 kb on + strand, within EX28DRAFT_3965 at 173.704 kb on + strand, within EX28DRAFT_3965 at 173.709 kb on + strand, within EX28DRAFT_3965 at 173.751 kb on + strand, within EX28DRAFT_3965 at 173.752 kb on - strand, within EX28DRAFT_3965 at 173.973 kb on - strand, within EX28DRAFT_3965 at 173.975 kb on - strand, within EX28DRAFT_3965 at 174.038 kb on + strand, within EX28DRAFT_3965 at 174.039 kb on - strand, within EX28DRAFT_3965 at 174.061 kb on + strand, within EX28DRAFT_3965 at 174.224 kb on - strand, within EX28DRAFT_3965 at 174.462 kb on - strand at 174.462 kb on - strand at 174.464 kb on + strand at 174.465 kb on - strand at 174.466 kb on + strand at 174.529 kb on + strand at 174.530 kb on - strand at 174.549 kb on + strand at 174.549 kb on + strand at 174.550 kb on - strand at 174.553 kb on - strand at 174.553 kb on - strand at 174.568 kb on - strand at 174.568 kb on - strand at 174.568 kb on - strand at 174.573 kb on + strand at 174.574 kb on - strand at 174.578 kb on + strand at 174.673 kb on + strand at 174.736 kb on + strand, within EX28DRAFT_3966 at 174.903 kb on + strand, within EX28DRAFT_3966 at 174.922 kb on + strand, within EX28DRAFT_3966 at 174.922 kb on + strand, within EX28DRAFT_3966 at 174.922 kb on + strand, within EX28DRAFT_3966 at 174.923 kb on - strand, within EX28DRAFT_3966 at 174.923 kb on - strand, within EX28DRAFT_3966 at 174.923 kb on - strand, within EX28DRAFT_3966 at 174.989 kb on - strand, within EX28DRAFT_3966 at 175.014 kb on + strand, within EX28DRAFT_3966 at 175.119 kb on + strand, within EX28DRAFT_3966 at 175.119 kb on + strand, within EX28DRAFT_3966 at 175.119 kb on + strand, within EX28DRAFT_3966 at 175.119 kb on + strand, within EX28DRAFT_3966 at 175.119 kb on + strand, within EX28DRAFT_3966 at 175.120 kb on - strand, within EX28DRAFT_3966 at 175.120 kb on - strand, within EX28DRAFT_3966 at 175.370 kb on - strand, within EX28DRAFT_3966 at 175.376 kb on - strand, within EX28DRAFT_3966 at 175.502 kb on - strand, within EX28DRAFT_3966
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 172,295 - EX28DRAFT_3964 0.25 -0.3 172,450 - EX28DRAFT_3964 0.41 +0.7 172,519 + EX28DRAFT_3964 0.49 -0.0 172,932 + -0.1 173,027 + -0.8 173,129 + -1.0 173,181 + -0.1 173,181 + +0.4 173,181 + -0.3 173,182 - +0.2 173,182 - -0.1 173,197 + -0.7 173,510 - EX28DRAFT_3965 0.23 +0.0 173,704 + EX28DRAFT_3965 0.38 +0.3 173,704 + EX28DRAFT_3965 0.38 +1.2 173,709 + EX28DRAFT_3965 0.38 +0.2 173,751 + EX28DRAFT_3965 0.41 -0.3 173,752 - EX28DRAFT_3965 0.41 -0.7 173,973 - EX28DRAFT_3965 0.58 +1.2 173,975 - EX28DRAFT_3965 0.58 +0.9 174,038 + EX28DRAFT_3965 0.63 +1.1 174,039 - EX28DRAFT_3965 0.63 -0.1 174,061 + EX28DRAFT_3965 0.64 -0.2 174,224 - EX28DRAFT_3965 0.77 -0.1 174,462 - +0.3 174,462 - +2.9 174,464 + -0.1 174,465 - +0.3 174,466 + -0.9 174,529 + -1.3 174,530 - -0.7 174,549 + -1.5 174,549 + -0.6 174,550 - +0.0 174,553 - -0.3 174,553 - +0.3 174,568 - -0.2 174,568 - -0.8 174,568 - -0.2 174,573 + -1.1 174,574 - -1.7 174,578 + -0.9 174,673 + +0.9 174,736 + EX28DRAFT_3966 0.14 -0.9 174,903 + EX28DRAFT_3966 0.27 -0.9 174,922 + EX28DRAFT_3966 0.29 -0.5 174,922 + EX28DRAFT_3966 0.29 -0.7 174,922 + EX28DRAFT_3966 0.29 -0.8 174,923 - EX28DRAFT_3966 0.29 -0.3 174,923 - EX28DRAFT_3966 0.29 -2.0 174,923 - EX28DRAFT_3966 0.29 +0.3 174,989 - EX28DRAFT_3966 0.34 +0.6 175,014 + EX28DRAFT_3966 0.36 +0.9 175,119 + EX28DRAFT_3966 0.44 -0.8 175,119 + EX28DRAFT_3966 0.44 -0.1 175,119 + EX28DRAFT_3966 0.44 -0.5 175,119 + EX28DRAFT_3966 0.44 -2.1 175,119 + EX28DRAFT_3966 0.44 -0.7 175,120 - EX28DRAFT_3966 0.44 -0.8 175,120 - EX28DRAFT_3966 0.44 +0.9 175,370 - EX28DRAFT_3966 0.63 +0.2 175,376 - EX28DRAFT_3966 0.64 +0.1 175,502 - EX28DRAFT_3966 0.74 +0.2
Or see this region's nucleotide sequence