Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3899 and EX28DRAFT_3900 are separated by 37 nucleotides EX28DRAFT_3900 and EX28DRAFT_3901 are separated by 3 nucleotides EX28DRAFT_3901 and EX28DRAFT_3902 are separated by 2 nucleotides
EX28DRAFT_3899: EX28DRAFT_3899 - (p)ppGpp synthetase, RelA/SpoT family, at 106,117 to 108,231
_3899
EX28DRAFT_3900: EX28DRAFT_3900 - SpoU rRNA Methylase family/SpoU, rRNA methylase, C-terminal, at 108,269 to 108,925
_3900
EX28DRAFT_3901: EX28DRAFT_3901 - ATP-dependent DNA helicase RecG (EC 3.6.1.-), at 108,929 to 111,010
_3901
EX28DRAFT_3902: EX28DRAFT_3902 - sodium--glutamate symport carrier (gltS), at 111,013 to 112,215
_3902
Position (kb)
108
109
110
111
112 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 107.977 kb on - strand, within EX28DRAFT_3899 at 108.010 kb on - strand, within EX28DRAFT_3899 at 108.018 kb on + strand, within EX28DRAFT_3899 at 108.018 kb on + strand, within EX28DRAFT_3899 at 108.018 kb on + strand, within EX28DRAFT_3899 at 108.171 kb on + strand at 108.230 kb on - strand at 108.230 kb on - strand at 108.251 kb on + strand at 108.259 kb on + strand at 108.319 kb on + strand at 108.319 kb on + strand at 108.319 kb on + strand at 108.320 kb on - strand at 108.320 kb on - strand at 108.711 kb on - strand, within EX28DRAFT_3900 at 108.746 kb on + strand, within EX28DRAFT_3900 at 108.747 kb on - strand, within EX28DRAFT_3900 at 109.109 kb on + strand at 109.325 kb on + strand, within EX28DRAFT_3901 at 109.558 kb on + strand, within EX28DRAFT_3901 at 109.566 kb on + strand, within EX28DRAFT_3901 at 109.614 kb on + strand, within EX28DRAFT_3901 at 109.681 kb on - strand, within EX28DRAFT_3901 at 109.730 kb on - strand, within EX28DRAFT_3901 at 109.879 kb on + strand, within EX28DRAFT_3901 at 110.027 kb on - strand, within EX28DRAFT_3901 at 110.256 kb on + strand, within EX28DRAFT_3901 at 110.257 kb on - strand, within EX28DRAFT_3901 at 110.260 kb on + strand, within EX28DRAFT_3901 at 110.399 kb on - strand, within EX28DRAFT_3901 at 110.399 kb on - strand, within EX28DRAFT_3901 at 110.462 kb on - strand, within EX28DRAFT_3901 at 111.038 kb on + strand at 111.039 kb on - strand at 111.218 kb on + strand, within EX28DRAFT_3902 at 111.583 kb on - strand, within EX28DRAFT_3902 at 111.583 kb on - strand, within EX28DRAFT_3902 at 111.583 kb on - strand, within EX28DRAFT_3902
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 107,977 - EX28DRAFT_3899 0.88 +0.1 108,010 - EX28DRAFT_3899 0.90 -0.9 108,018 + EX28DRAFT_3899 0.90 +1.0 108,018 + EX28DRAFT_3899 0.90 +0.0 108,018 + EX28DRAFT_3899 0.90 +0.4 108,171 + -0.0 108,230 - +1.2 108,230 - -0.6 108,251 + -0.1 108,259 + -0.1 108,319 + +0.3 108,319 + +0.6 108,319 + +2.1 108,320 - +0.4 108,320 - -0.3 108,711 - EX28DRAFT_3900 0.67 +1.1 108,746 + EX28DRAFT_3900 0.73 -0.5 108,747 - EX28DRAFT_3900 0.73 -0.2 109,109 + -0.7 109,325 + EX28DRAFT_3901 0.19 -1.3 109,558 + EX28DRAFT_3901 0.30 -2.3 109,566 + EX28DRAFT_3901 0.31 -0.9 109,614 + EX28DRAFT_3901 0.33 -1.0 109,681 - EX28DRAFT_3901 0.36 -0.8 109,730 - EX28DRAFT_3901 0.38 -0.5 109,879 + EX28DRAFT_3901 0.46 -4.1 110,027 - EX28DRAFT_3901 0.53 +0.7 110,256 + EX28DRAFT_3901 0.64 +0.4 110,257 - EX28DRAFT_3901 0.64 -0.5 110,260 + EX28DRAFT_3901 0.64 -0.7 110,399 - EX28DRAFT_3901 0.71 -0.5 110,399 - EX28DRAFT_3901 0.71 -0.6 110,462 - EX28DRAFT_3901 0.74 -1.0 111,038 + -0.0 111,039 - +0.4 111,218 + EX28DRAFT_3902 0.17 -0.9 111,583 - EX28DRAFT_3902 0.47 +0.4 111,583 - EX28DRAFT_3902 0.47 -0.5 111,583 - EX28DRAFT_3902 0.47 +0.2
Or see this region's nucleotide sequence