Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3811 and EX28DRAFT_3812 are separated by 163 nucleotides EX28DRAFT_3812 and EX28DRAFT_3813 are separated by 129 nucleotides EX28DRAFT_3813 and EX28DRAFT_3814 are separated by 118 nucleotides EX28DRAFT_3814 and EX28DRAFT_3815 are separated by 3 nucleotides
EX28DRAFT_3811: EX28DRAFT_3811 - molybdopterin guanine dinucleotide-containing S/N-oxide reductases, at 14,365 to 16,692
_3811
EX28DRAFT_3812: EX28DRAFT_3812 - Outer membrane protein and related peptidoglycan-associated (lipo)proteins, at 16,856 to 17,518
_3812
EX28DRAFT_3813: EX28DRAFT_3813 - Transcriptional regulators, at 17,648 to 18,664
_3813
EX28DRAFT_3814: EX28DRAFT_3814 - hypothetical protein, at 18,783 to 19,517
_3814
EX28DRAFT_3815: EX28DRAFT_3815 - Sugar kinases, ribokinase family, at 19,521 to 20,459
_3815
Position (kb) 17
18
19 Strain fitness (log2 ratio) -2
-1
0
1 at 16.721 kb on - strand at 16.745 kb on - strand at 16.769 kb on + strand at 16.769 kb on + strand at 16.802 kb on + strand at 16.803 kb on - strand at 16.869 kb on + strand at 17.093 kb on + strand, within EX28DRAFT_3812 at 17.093 kb on + strand, within EX28DRAFT_3812 at 17.094 kb on - strand, within EX28DRAFT_3812 at 17.094 kb on - strand, within EX28DRAFT_3812 at 17.182 kb on + strand, within EX28DRAFT_3812 at 17.277 kb on - strand, within EX28DRAFT_3812 at 17.497 kb on + strand at 17.516 kb on + strand at 17.619 kb on + strand at 17.713 kb on + strand at 17.770 kb on + strand, within EX28DRAFT_3813 at 17.950 kb on + strand, within EX28DRAFT_3813 at 17.954 kb on - strand, within EX28DRAFT_3813 at 17.985 kb on - strand, within EX28DRAFT_3813 at 18.005 kb on + strand, within EX28DRAFT_3813 at 18.006 kb on - strand, within EX28DRAFT_3813 at 18.006 kb on - strand, within EX28DRAFT_3813 at 18.085 kb on + strand, within EX28DRAFT_3813 at 18.086 kb on - strand, within EX28DRAFT_3813 at 18.687 kb on + strand at 18.688 kb on - strand at 18.734 kb on + strand at 18.734 kb on + strand at 18.738 kb on - strand at 18.741 kb on - strand at 18.746 kb on + strand at 18.746 kb on + strand at 18.747 kb on - strand at 18.747 kb on - strand at 18.783 kb on - strand at 18.973 kb on - strand, within EX28DRAFT_3814 at 18.996 kb on - strand, within EX28DRAFT_3814 at 19.121 kb on + strand, within EX28DRAFT_3814 at 19.323 kb on + strand, within EX28DRAFT_3814 at 19.324 kb on - strand, within EX28DRAFT_3814 at 19.324 kb on - strand, within EX28DRAFT_3814 at 19.324 kb on - strand, within EX28DRAFT_3814 at 19.324 kb on - strand, within EX28DRAFT_3814 at 19.324 kb on - strand, within EX28DRAFT_3814 at 19.359 kb on - strand, within EX28DRAFT_3814 at 19.515 kb on + strand at 19.613 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 16,721 - -1.1 16,745 - +0.5 16,769 + -0.9 16,769 + +0.1 16,802 + -0.4 16,803 - -0.3 16,869 + +0.4 17,093 + EX28DRAFT_3812 0.36 +0.4 17,093 + EX28DRAFT_3812 0.36 +0.5 17,094 - EX28DRAFT_3812 0.36 +1.3 17,094 - EX28DRAFT_3812 0.36 +0.1 17,182 + EX28DRAFT_3812 0.49 -0.2 17,277 - EX28DRAFT_3812 0.63 +0.2 17,497 + +0.1 17,516 + +0.2 17,619 + -0.0 17,713 + +0.6 17,770 + EX28DRAFT_3813 0.12 -0.7 17,950 + EX28DRAFT_3813 0.30 +0.3 17,954 - EX28DRAFT_3813 0.30 -0.6 17,985 - EX28DRAFT_3813 0.33 +0.5 18,005 + EX28DRAFT_3813 0.35 +0.4 18,006 - EX28DRAFT_3813 0.35 +1.7 18,006 - EX28DRAFT_3813 0.35 +0.9 18,085 + EX28DRAFT_3813 0.43 +0.4 18,086 - EX28DRAFT_3813 0.43 -0.0 18,687 + -0.3 18,688 - +0.3 18,734 + -0.2 18,734 + +1.1 18,738 - -0.8 18,741 - -1.2 18,746 + -0.1 18,746 + -0.1 18,747 - +0.5 18,747 - +0.5 18,783 - +0.2 18,973 - EX28DRAFT_3814 0.26 +0.2 18,996 - EX28DRAFT_3814 0.29 +0.7 19,121 + EX28DRAFT_3814 0.46 -0.4 19,323 + EX28DRAFT_3814 0.73 +0.2 19,324 - EX28DRAFT_3814 0.74 +1.0 19,324 - EX28DRAFT_3814 0.74 -0.9 19,324 - EX28DRAFT_3814 0.74 -1.9 19,324 - EX28DRAFT_3814 0.74 -0.0 19,324 - EX28DRAFT_3814 0.74 +0.5 19,359 - EX28DRAFT_3814 0.78 -0.3 19,515 + +0.2 19,613 + +0.4
Or see this region's nucleotide sequence