Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3800 and EX28DRAFT_3801 overlap by 4 nucleotides EX28DRAFT_3801 and EX28DRAFT_3802 are separated by 10 nucleotides EX28DRAFT_3802 and EX28DRAFT_3803 are separated by 9 nucleotides
EX28DRAFT_3800: EX28DRAFT_3800 - oligopeptide/dipeptide ABC transporter, ATP-binding protein, C-terminal domain, at 2,676 to 3,689
_3800
EX28DRAFT_3801: EX28DRAFT_3801 - oligopeptide/dipeptide ABC transporter, ATP-binding protein, C-terminal domain, at 3,686 to 4,669
_3801
EX28DRAFT_3802: EX28DRAFT_3802 - ABC-type dipeptide/oligopeptide/nickel transport systems, permease components, at 4,680 to 5,582
_3802
EX28DRAFT_3803: EX28DRAFT_3803 - ABC-type dipeptide/oligopeptide/nickel transport systems, permease components, at 5,592 to 6,611
_3803
Position (kb) 4
5
6 Strain fitness (log2 ratio) -1
0
1
2 at 3.683 kb on + strand at 3.689 kb on + strand at 3.690 kb on - strand at 3.837 kb on + strand, within EX28DRAFT_3801 at 3.837 kb on + strand, within EX28DRAFT_3801 at 3.838 kb on - strand, within EX28DRAFT_3801 at 3.838 kb on - strand, within EX28DRAFT_3801 at 3.838 kb on - strand, within EX28DRAFT_3801 at 4.058 kb on - strand, within EX28DRAFT_3801 at 4.301 kb on - strand, within EX28DRAFT_3801 at 4.353 kb on + strand, within EX28DRAFT_3801 at 4.459 kb on + strand, within EX28DRAFT_3801 at 4.580 kb on + strand at 4.580 kb on + strand at 4.581 kb on - strand at 4.659 kb on - strand at 4.681 kb on + strand at 4.733 kb on + strand at 4.734 kb on - strand at 4.734 kb on - strand at 4.943 kb on + strand, within EX28DRAFT_3802 at 4.943 kb on + strand, within EX28DRAFT_3802 at 4.943 kb on + strand, within EX28DRAFT_3802 at 4.952 kb on + strand, within EX28DRAFT_3802 at 5.056 kb on + strand, within EX28DRAFT_3802 at 5.056 kb on + strand, within EX28DRAFT_3802 at 5.057 kb on - strand, within EX28DRAFT_3802 at 5.229 kb on + strand, within EX28DRAFT_3802 at 5.230 kb on - strand, within EX28DRAFT_3802 at 5.297 kb on - strand, within EX28DRAFT_3802 at 5.419 kb on + strand, within EX28DRAFT_3802 at 5.420 kb on - strand, within EX28DRAFT_3802 at 5.463 kb on + strand, within EX28DRAFT_3802 at 5.464 kb on - strand, within EX28DRAFT_3802 at 5.464 kb on - strand, within EX28DRAFT_3802 at 5.470 kb on + strand, within EX28DRAFT_3802 at 5.471 kb on - strand, within EX28DRAFT_3802 at 5.562 kb on - strand at 5.562 kb on - strand at 5.593 kb on + strand at 5.594 kb on - strand at 5.600 kb on + strand at 5.600 kb on + strand at 5.601 kb on - strand at 5.630 kb on - strand at 5.683 kb on - strand at 5.683 kb on - strand at 5.683 kb on - strand at 5.840 kb on + strand, within EX28DRAFT_3803 at 5.903 kb on + strand, within EX28DRAFT_3803 at 5.905 kb on + strand, within EX28DRAFT_3803 at 6.041 kb on + strand, within EX28DRAFT_3803 at 6.060 kb on - strand, within EX28DRAFT_3803 at 6.098 kb on + strand, within EX28DRAFT_3803 at 6.098 kb on + strand, within EX28DRAFT_3803 at 6.179 kb on + strand, within EX28DRAFT_3803 at 6.378 kb on + strand, within EX28DRAFT_3803 at 6.404 kb on + strand, within EX28DRAFT_3803 at 6.404 kb on + strand, within EX28DRAFT_3803 at 6.404 kb on + strand, within EX28DRAFT_3803 at 6.404 kb on + strand, within EX28DRAFT_3803 at 6.405 kb on - strand, within EX28DRAFT_3803 at 6.405 kb on - strand, within EX28DRAFT_3803 at 6.406 kb on + strand, within EX28DRAFT_3803 at 6.406 kb on + strand, within EX28DRAFT_3803 at 6.407 kb on - strand, within EX28DRAFT_3803 at 6.407 kb on - strand, within EX28DRAFT_3803 at 6.482 kb on + strand, within EX28DRAFT_3803 at 6.500 kb on + strand, within EX28DRAFT_3803 at 6.501 kb on - strand, within EX28DRAFT_3803 at 6.527 kb on + strand at 6.528 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 3,683 + +0.6 3,689 + +0.0 3,690 - +1.5 3,837 + EX28DRAFT_3801 0.15 +0.1 3,837 + EX28DRAFT_3801 0.15 -0.3 3,838 - EX28DRAFT_3801 0.15 -0.1 3,838 - EX28DRAFT_3801 0.15 +0.5 3,838 - EX28DRAFT_3801 0.15 -0.5 4,058 - EX28DRAFT_3801 0.38 +0.8 4,301 - EX28DRAFT_3801 0.62 +0.7 4,353 + EX28DRAFT_3801 0.68 +0.2 4,459 + EX28DRAFT_3801 0.79 +0.3 4,580 + +0.6 4,580 + +0.6 4,581 - +0.2 4,659 - +0.3 4,681 + +0.7 4,733 + +0.6 4,734 - +0.3 4,734 - +0.4 4,943 + EX28DRAFT_3802 0.29 -0.1 4,943 + EX28DRAFT_3802 0.29 +0.3 4,943 + EX28DRAFT_3802 0.29 +0.2 4,952 + EX28DRAFT_3802 0.30 -0.7 5,056 + EX28DRAFT_3802 0.42 +0.6 5,056 + EX28DRAFT_3802 0.42 +0.0 5,057 - EX28DRAFT_3802 0.42 -1.4 5,229 + EX28DRAFT_3802 0.61 -0.5 5,230 - EX28DRAFT_3802 0.61 -0.8 5,297 - EX28DRAFT_3802 0.68 +0.2 5,419 + EX28DRAFT_3802 0.82 -0.5 5,420 - EX28DRAFT_3802 0.82 -0.3 5,463 + EX28DRAFT_3802 0.87 -0.7 5,464 - EX28DRAFT_3802 0.87 -0.3 5,464 - EX28DRAFT_3802 0.87 -0.7 5,470 + EX28DRAFT_3802 0.87 +0.3 5,471 - EX28DRAFT_3802 0.88 -0.4 5,562 - -0.0 5,562 - +0.2 5,593 + -0.1 5,594 - -0.1 5,600 + +0.6 5,600 + +0.9 5,601 - -1.4 5,630 - -0.7 5,683 - +0.5 5,683 - -0.3 5,683 - -0.2 5,840 + EX28DRAFT_3803 0.24 +0.4 5,903 + EX28DRAFT_3803 0.30 +0.6 5,905 + EX28DRAFT_3803 0.31 -0.5 6,041 + EX28DRAFT_3803 0.44 +0.1 6,060 - EX28DRAFT_3803 0.46 +0.2 6,098 + EX28DRAFT_3803 0.50 +1.0 6,098 + EX28DRAFT_3803 0.50 +0.7 6,179 + EX28DRAFT_3803 0.58 +0.2 6,378 + EX28DRAFT_3803 0.77 -0.7 6,404 + EX28DRAFT_3803 0.80 -1.0 6,404 + EX28DRAFT_3803 0.80 +0.8 6,404 + EX28DRAFT_3803 0.80 -0.2 6,404 + EX28DRAFT_3803 0.80 +0.1 6,405 - EX28DRAFT_3803 0.80 +0.4 6,405 - EX28DRAFT_3803 0.80 -0.1 6,406 + EX28DRAFT_3803 0.80 +0.5 6,406 + EX28DRAFT_3803 0.80 +0.4 6,407 - EX28DRAFT_3803 0.80 +0.6 6,407 - EX28DRAFT_3803 0.80 +0.0 6,482 + EX28DRAFT_3803 0.87 -0.0 6,500 + EX28DRAFT_3803 0.89 +2.7 6,501 - EX28DRAFT_3803 0.89 -0.1 6,527 + -0.2 6,528 - -0.0
Or see this region's nucleotide sequence