Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3733 and EX28DRAFT_3734 are separated by 59 nucleotides EX28DRAFT_3734 and EX28DRAFT_3735 are separated by 176 nucleotides EX28DRAFT_3735 and EX28DRAFT_3736 overlap by 11 nucleotides EX28DRAFT_3736 and EX28DRAFT_3737 are separated by 73 nucleotides
EX28DRAFT_3733: EX28DRAFT_3733 - glutamyl-queuosine tRNA(Asp) synthetase, at 408,021 to 408,911
_3733
EX28DRAFT_3734: EX28DRAFT_3734 - transcriptional regulator, TraR/DksA family, at 408,971 to 409,426
_3734
EX28DRAFT_3735: EX28DRAFT_3735 - sugar fermentation stimulation protein, at 409,603 to 410,307
_3735
EX28DRAFT_3736: EX28DRAFT_3736 - 2'-5' RNA ligase, at 410,297 to 410,851
_3736
EX28DRAFT_3737: EX28DRAFT_3737 - ATP-dependent helicase HrpB, at 410,925 to 413,354
_3737
Position (kb) 409
410
411 Strain fitness (log2 ratio) -1
0
1
2 at 408.616 kb on + strand, within EX28DRAFT_3733 at 408.616 kb on + strand, within EX28DRAFT_3733 at 408.616 kb on + strand, within EX28DRAFT_3733 at 408.617 kb on - strand, within EX28DRAFT_3733 at 408.823 kb on + strand at 408.843 kb on + strand at 408.859 kb on - strand at 408.859 kb on - strand at 408.894 kb on - strand at 408.895 kb on + strand at 408.896 kb on - strand at 408.896 kb on - strand at 408.911 kb on + strand at 408.911 kb on + strand at 408.911 kb on + strand at 408.957 kb on - strand at 408.969 kb on + strand at 408.970 kb on - strand at 409.487 kb on - strand at 409.487 kb on - strand at 409.487 kb on - strand at 409.487 kb on - strand at 409.491 kb on - strand at 409.491 kb on - strand at 409.517 kb on - strand at 409.521 kb on - strand at 409.521 kb on - strand at 409.526 kb on + strand at 409.527 kb on - strand at 409.604 kb on + strand at 409.606 kb on + strand at 409.607 kb on - strand at 409.607 kb on - strand at 409.607 kb on - strand at 409.639 kb on - strand at 409.639 kb on - strand at 409.639 kb on - strand at 409.662 kb on + strand at 409.720 kb on - strand, within EX28DRAFT_3735 at 409.816 kb on + strand, within EX28DRAFT_3735 at 409.817 kb on - strand, within EX28DRAFT_3735 at 409.910 kb on + strand, within EX28DRAFT_3735 at 409.911 kb on - strand, within EX28DRAFT_3735 at 409.970 kb on + strand, within EX28DRAFT_3735 at 409.970 kb on + strand, within EX28DRAFT_3735 at 409.986 kb on + strand, within EX28DRAFT_3735 at 409.986 kb on + strand, within EX28DRAFT_3735 at 410.077 kb on - strand, within EX28DRAFT_3735 at 410.077 kb on - strand, within EX28DRAFT_3735 at 410.112 kb on + strand, within EX28DRAFT_3735 at 410.113 kb on - strand, within EX28DRAFT_3735 at 410.120 kb on + strand, within EX28DRAFT_3735 at 410.120 kb on + strand, within EX28DRAFT_3735 at 410.120 kb on + strand, within EX28DRAFT_3735 at 410.133 kb on + strand, within EX28DRAFT_3735 at 410.476 kb on + strand, within EX28DRAFT_3736 at 410.477 kb on - strand, within EX28DRAFT_3736 at 410.872 kb on - strand at 410.872 kb on - strand at 410.884 kb on + strand at 410.892 kb on - strand at 410.907 kb on - strand at 410.974 kb on + strand at 410.975 kb on - strand at 410.975 kb on - strand at 411.203 kb on + strand, within EX28DRAFT_3737 at 411.231 kb on - strand, within EX28DRAFT_3737
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 408,616 + EX28DRAFT_3733 0.67 +0.1 408,616 + EX28DRAFT_3733 0.67 +0.1 408,616 + EX28DRAFT_3733 0.67 +1.2 408,617 - EX28DRAFT_3733 0.67 +0.4 408,823 + +0.7 408,843 + -1.0 408,859 - +1.2 408,859 - +0.0 408,894 - +0.2 408,895 + +0.5 408,896 - -0.1 408,896 - +0.7 408,911 + -0.1 408,911 + +0.2 408,911 + -1.7 408,957 - +0.6 408,969 + +0.1 408,970 - -0.7 409,487 - +0.3 409,487 - +0.0 409,487 - +0.1 409,487 - +0.4 409,491 - +0.3 409,491 - +0.4 409,517 - +0.5 409,521 - +1.0 409,521 - +0.0 409,526 + +1.2 409,527 - +0.1 409,604 + -0.5 409,606 + +0.9 409,607 - +0.1 409,607 - +0.1 409,607 - -1.7 409,639 - -0.3 409,639 - +0.9 409,639 - -0.3 409,662 + +0.9 409,720 - EX28DRAFT_3735 0.17 +0.3 409,816 + EX28DRAFT_3735 0.30 +0.5 409,817 - EX28DRAFT_3735 0.30 -0.3 409,910 + EX28DRAFT_3735 0.44 -0.2 409,911 - EX28DRAFT_3735 0.44 +1.6 409,970 + EX28DRAFT_3735 0.52 +1.3 409,970 + EX28DRAFT_3735 0.52 +1.2 409,986 + EX28DRAFT_3735 0.54 -0.7 409,986 + EX28DRAFT_3735 0.54 +1.2 410,077 - EX28DRAFT_3735 0.67 -0.3 410,077 - EX28DRAFT_3735 0.67 -0.9 410,112 + EX28DRAFT_3735 0.72 -0.8 410,113 - EX28DRAFT_3735 0.72 -0.1 410,120 + EX28DRAFT_3735 0.73 -1.0 410,120 + EX28DRAFT_3735 0.73 +2.1 410,120 + EX28DRAFT_3735 0.73 +0.5 410,133 + EX28DRAFT_3735 0.75 +0.5 410,476 + EX28DRAFT_3736 0.32 -0.1 410,477 - EX28DRAFT_3736 0.32 -0.4 410,872 - -0.1 410,872 - +0.3 410,884 + +1.0 410,892 - +0.2 410,907 - +0.4 410,974 + +0.4 410,975 - -0.6 410,975 - -0.2 411,203 + EX28DRAFT_3737 0.11 +0.3 411,231 - EX28DRAFT_3737 0.13 -1.0
Or see this region's nucleotide sequence