Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3587 and EX28DRAFT_3588 overlap by 6 nucleotides EX28DRAFT_3588 and EX28DRAFT_3589 overlap by 26 nucleotides EX28DRAFT_3589 and EX28DRAFT_3590 are separated by 262 nucleotides
EX28DRAFT_3587: EX28DRAFT_3587 - Mg-dependent DNase, at 243,880 to 244,662
_3587
EX28DRAFT_3588: EX28DRAFT_3588 - glycine radical enzyme activase, YjjW family, at 244,657 to 245,517
_3588
EX28DRAFT_3589: EX28DRAFT_3589 - glycine radical enzyme, YjjI family, at 245,492 to 247,039
_3589
EX28DRAFT_3590: EX28DRAFT_3590 - deoxyribose-phosphate aldolase, at 247,302 to 248,081
_3590
Position (kb) 245
246
247
248 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 244.660 kb on + strand at 244.660 kb on + strand at 244.664 kb on + strand at 244.664 kb on + strand at 244.665 kb on - strand at 244.665 kb on - strand at 244.687 kb on + strand at 244.687 kb on + strand at 244.876 kb on - strand, within EX28DRAFT_3588 at 244.935 kb on + strand, within EX28DRAFT_3588 at 244.936 kb on - strand, within EX28DRAFT_3588 at 245.120 kb on - strand, within EX28DRAFT_3588 at 245.241 kb on + strand, within EX28DRAFT_3588 at 245.584 kb on + strand at 245.587 kb on + strand at 245.642 kb on - strand at 245.643 kb on + strand at 245.644 kb on - strand at 245.776 kb on + strand, within EX28DRAFT_3589 at 245.797 kb on - strand, within EX28DRAFT_3589 at 245.797 kb on - strand, within EX28DRAFT_3589 at 245.797 kb on - strand, within EX28DRAFT_3589 at 245.797 kb on - strand, within EX28DRAFT_3589 at 246.059 kb on - strand, within EX28DRAFT_3589 at 246.081 kb on + strand, within EX28DRAFT_3589 at 246.081 kb on + strand, within EX28DRAFT_3589 at 246.082 kb on - strand, within EX28DRAFT_3589 at 246.142 kb on + strand, within EX28DRAFT_3589 at 246.143 kb on - strand, within EX28DRAFT_3589 at 246.143 kb on - strand, within EX28DRAFT_3589 at 246.417 kb on - strand, within EX28DRAFT_3589 at 246.468 kb on + strand, within EX28DRAFT_3589 at 246.662 kb on + strand, within EX28DRAFT_3589 at 246.665 kb on + strand, within EX28DRAFT_3589 at 246.666 kb on - strand, within EX28DRAFT_3589 at 246.687 kb on + strand, within EX28DRAFT_3589 at 246.688 kb on - strand, within EX28DRAFT_3589 at 246.828 kb on + strand, within EX28DRAFT_3589 at 246.872 kb on - strand, within EX28DRAFT_3589 at 246.975 kb on - strand at 247.039 kb on + strand at 247.039 kb on + strand at 247.040 kb on - strand at 247.080 kb on + strand at 247.131 kb on + strand at 247.249 kb on + strand at 247.275 kb on + strand at 247.301 kb on + strand at 247.313 kb on - strand at 247.373 kb on + strand at 247.374 kb on - strand at 247.443 kb on - strand, within EX28DRAFT_3590 at 247.443 kb on - strand, within EX28DRAFT_3590 at 247.519 kb on + strand, within EX28DRAFT_3590 at 247.523 kb on + strand, within EX28DRAFT_3590 at 247.523 kb on + strand, within EX28DRAFT_3590 at 247.524 kb on - strand, within EX28DRAFT_3590 at 247.677 kb on - strand, within EX28DRAFT_3590 at 247.758 kb on - strand, within EX28DRAFT_3590 at 247.999 kb on - strand, within EX28DRAFT_3590
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 244,660 + -0.2 244,660 + +0.5 244,664 + -0.1 244,664 + +1.3 244,665 - +1.4 244,665 - +0.3 244,687 + +1.7 244,687 + +0.3 244,876 - EX28DRAFT_3588 0.25 +0.1 244,935 + EX28DRAFT_3588 0.32 +0.5 244,936 - EX28DRAFT_3588 0.32 +0.6 245,120 - EX28DRAFT_3588 0.54 -0.1 245,241 + EX28DRAFT_3588 0.68 +0.7 245,584 + -0.6 245,587 + -0.2 245,642 - -0.7 245,643 + -0.2 245,644 - +0.6 245,776 + EX28DRAFT_3589 0.18 -0.2 245,797 - EX28DRAFT_3589 0.20 -0.6 245,797 - EX28DRAFT_3589 0.20 +0.5 245,797 - EX28DRAFT_3589 0.20 -0.3 245,797 - EX28DRAFT_3589 0.20 -1.5 246,059 - EX28DRAFT_3589 0.37 -0.3 246,081 + EX28DRAFT_3589 0.38 -2.0 246,081 + EX28DRAFT_3589 0.38 -0.5 246,082 - EX28DRAFT_3589 0.38 -0.2 246,142 + EX28DRAFT_3589 0.42 -1.5 246,143 - EX28DRAFT_3589 0.42 +0.3 246,143 - EX28DRAFT_3589 0.42 -0.3 246,417 - EX28DRAFT_3589 0.60 -0.4 246,468 + EX28DRAFT_3589 0.63 -0.1 246,662 + EX28DRAFT_3589 0.76 -0.5 246,665 + EX28DRAFT_3589 0.76 -0.2 246,666 - EX28DRAFT_3589 0.76 -0.2 246,687 + EX28DRAFT_3589 0.77 +0.7 246,688 - EX28DRAFT_3589 0.77 -0.7 246,828 + EX28DRAFT_3589 0.86 -0.7 246,872 - EX28DRAFT_3589 0.89 -0.1 246,975 - +1.1 247,039 + -1.1 247,039 + -0.7 247,040 - +1.0 247,080 + +0.0 247,131 + +0.0 247,249 + +0.4 247,275 + +0.5 247,301 + -2.8 247,313 - +0.6 247,373 + -0.5 247,374 - -0.6 247,443 - EX28DRAFT_3590 0.18 -0.7 247,443 - EX28DRAFT_3590 0.18 -0.6 247,519 + EX28DRAFT_3590 0.28 +0.3 247,523 + EX28DRAFT_3590 0.28 -0.3 247,523 + EX28DRAFT_3590 0.28 -0.5 247,524 - EX28DRAFT_3590 0.28 +0.3 247,677 - EX28DRAFT_3590 0.48 +0.2 247,758 - EX28DRAFT_3590 0.58 +0.9 247,999 - EX28DRAFT_3590 0.89 +1.6
Or see this region's nucleotide sequence