Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3583 and EX28DRAFT_3584 are separated by 299 nucleotides EX28DRAFT_3584 and EX28DRAFT_3585 are separated by 109 nucleotides EX28DRAFT_3585 and EX28DRAFT_3586 are separated by 122 nucleotides
EX28DRAFT_3583: EX28DRAFT_3583 - peptide chain release factor 3, at 239,913 to 241,502
_3583
EX28DRAFT_3584: EX28DRAFT_3584 - Predicted periplasmic or secreted lipoprotein, at 241,802 to 242,419
_3584
EX28DRAFT_3585: EX28DRAFT_3585 - Small integral membrane protein, at 242,529 to 242,708
_3585
EX28DRAFT_3586: EX28DRAFT_3586 - Predicted esterase of the alpha-beta hydrolase superfamily, at 242,831 to 243,883
_3586
Position (kb)
241
242
243 Strain fitness (log2 ratio)
-2
-1
0
1 at 240.881 kb on + strand, within EX28DRAFT_3583 at 241.367 kb on + strand at 241.419 kb on + strand at 241.419 kb on + strand at 241.419 kb on + strand at 241.420 kb on - strand at 241.500 kb on + strand at 241.576 kb on + strand at 241.647 kb on + strand at 241.647 kb on + strand at 241.648 kb on - strand at 241.653 kb on + strand at 241.686 kb on + strand at 241.746 kb on + strand at 241.764 kb on - strand at 241.764 kb on - strand at 241.764 kb on - strand at 241.767 kb on + strand at 241.951 kb on + strand, within EX28DRAFT_3584 at 241.957 kb on + strand, within EX28DRAFT_3584 at 241.957 kb on + strand, within EX28DRAFT_3584 at 241.958 kb on - strand, within EX28DRAFT_3584 at 241.958 kb on - strand, within EX28DRAFT_3584 at 241.958 kb on - strand, within EX28DRAFT_3584 at 242.175 kb on + strand, within EX28DRAFT_3584 at 242.175 kb on + strand, within EX28DRAFT_3584 at 242.176 kb on - strand, within EX28DRAFT_3584 at 242.176 kb on - strand, within EX28DRAFT_3584 at 242.207 kb on + strand, within EX28DRAFT_3584 at 242.227 kb on + strand, within EX28DRAFT_3584 at 242.227 kb on + strand, within EX28DRAFT_3584 at 242.289 kb on + strand, within EX28DRAFT_3584 at 242.289 kb on + strand, within EX28DRAFT_3584 at 242.289 kb on + strand, within EX28DRAFT_3584 at 242.289 kb on + strand, within EX28DRAFT_3584 at 242.418 kb on - strand at 242.462 kb on + strand at 242.463 kb on - strand at 242.463 kb on - strand at 242.524 kb on + strand at 242.525 kb on - strand at 242.525 kb on - strand at 242.528 kb on + strand at 242.528 kb on + strand at 242.528 kb on + strand at 242.528 kb on + strand at 242.529 kb on - strand at 242.529 kb on - strand at 242.529 kb on - strand at 242.534 kb on - strand at 242.546 kb on + strand at 242.547 kb on - strand, within EX28DRAFT_3585 at 242.547 kb on - strand, within EX28DRAFT_3585 at 242.564 kb on + strand, within EX28DRAFT_3585 at 242.564 kb on + strand, within EX28DRAFT_3585 at 242.564 kb on + strand, within EX28DRAFT_3585 at 242.564 kb on + strand, within EX28DRAFT_3585 at 242.566 kb on + strand, within EX28DRAFT_3585 at 242.567 kb on - strand, within EX28DRAFT_3585 at 242.567 kb on - strand, within EX28DRAFT_3585 at 242.567 kb on - strand, within EX28DRAFT_3585 at 242.621 kb on + strand, within EX28DRAFT_3585 at 242.622 kb on - strand, within EX28DRAFT_3585 at 242.663 kb on + strand, within EX28DRAFT_3585 at 242.663 kb on + strand, within EX28DRAFT_3585 at 242.697 kb on - strand at 242.718 kb on + strand at 242.718 kb on + strand at 242.803 kb on - strand at 242.999 kb on + strand, within EX28DRAFT_3586 at 243.038 kb on + strand, within EX28DRAFT_3586 at 243.039 kb on - strand, within EX28DRAFT_3586 at 243.046 kb on + strand, within EX28DRAFT_3586 at 243.047 kb on - strand, within EX28DRAFT_3586 at 243.047 kb on - strand, within EX28DRAFT_3586 at 243.260 kb on + strand, within EX28DRAFT_3586 at 243.338 kb on + strand, within EX28DRAFT_3586 at 243.338 kb on + strand, within EX28DRAFT_3586 at 243.367 kb on + strand, within EX28DRAFT_3586 at 243.368 kb on - strand, within EX28DRAFT_3586 at 243.368 kb on - strand, within EX28DRAFT_3586 at 243.371 kb on + strand, within EX28DRAFT_3586
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 240,881 + EX28DRAFT_3583 0.61 -0.3 241,367 + -1.5 241,419 + -0.9 241,419 + +0.2 241,419 + -0.5 241,420 - +1.5 241,500 + +0.3 241,576 + -2.3 241,647 + +0.3 241,647 + +0.7 241,648 - +0.3 241,653 + -0.7 241,686 + -0.2 241,746 + +0.5 241,764 - -0.3 241,764 - +0.1 241,764 - -0.1 241,767 + -0.3 241,951 + EX28DRAFT_3584 0.24 +0.3 241,957 + EX28DRAFT_3584 0.25 +0.6 241,957 + EX28DRAFT_3584 0.25 +0.0 241,958 - EX28DRAFT_3584 0.25 -0.7 241,958 - EX28DRAFT_3584 0.25 -1.3 241,958 - EX28DRAFT_3584 0.25 -1.2 242,175 + EX28DRAFT_3584 0.60 -1.3 242,175 + EX28DRAFT_3584 0.60 +1.7 242,176 - EX28DRAFT_3584 0.61 +0.3 242,176 - EX28DRAFT_3584 0.61 -0.2 242,207 + EX28DRAFT_3584 0.66 -0.9 242,227 + EX28DRAFT_3584 0.69 +0.6 242,227 + EX28DRAFT_3584 0.69 +0.4 242,289 + EX28DRAFT_3584 0.79 -0.3 242,289 + EX28DRAFT_3584 0.79 +0.8 242,289 + EX28DRAFT_3584 0.79 -0.9 242,289 + EX28DRAFT_3584 0.79 -0.0 242,418 - +0.2 242,462 + +0.1 242,463 - -0.3 242,463 - +0.3 242,524 + +0.2 242,525 - -1.7 242,525 - +1.5 242,528 + -0.5 242,528 + +1.3 242,528 + -0.6 242,528 + -0.0 242,529 - -1.1 242,529 - -0.2 242,529 - +0.5 242,534 - +0.9 242,546 + +0.8 242,547 - EX28DRAFT_3585 0.10 +0.2 242,547 - EX28DRAFT_3585 0.10 +0.3 242,564 + EX28DRAFT_3585 0.19 -0.6 242,564 + EX28DRAFT_3585 0.19 +0.3 242,564 + EX28DRAFT_3585 0.19 +0.7 242,564 + EX28DRAFT_3585 0.19 -0.3 242,566 + EX28DRAFT_3585 0.21 +0.2 242,567 - EX28DRAFT_3585 0.21 -0.9 242,567 - EX28DRAFT_3585 0.21 -0.5 242,567 - EX28DRAFT_3585 0.21 +0.5 242,621 + EX28DRAFT_3585 0.51 -2.0 242,622 - EX28DRAFT_3585 0.52 -0.3 242,663 + EX28DRAFT_3585 0.74 -0.4 242,663 + EX28DRAFT_3585 0.74 +0.3 242,697 - +1.0 242,718 + +0.1 242,718 + -0.6 242,803 - -0.1 242,999 + EX28DRAFT_3586 0.16 -0.2 243,038 + EX28DRAFT_3586 0.20 -0.9 243,039 - EX28DRAFT_3586 0.20 -2.1 243,046 + EX28DRAFT_3586 0.20 +0.3 243,047 - EX28DRAFT_3586 0.21 +0.4 243,047 - EX28DRAFT_3586 0.21 -0.2 243,260 + EX28DRAFT_3586 0.41 +0.1 243,338 + EX28DRAFT_3586 0.48 +1.5 243,338 + EX28DRAFT_3586 0.48 -0.1 243,367 + EX28DRAFT_3586 0.51 +0.4 243,368 - EX28DRAFT_3586 0.51 -0.2 243,368 - EX28DRAFT_3586 0.51 +1.1 243,371 + EX28DRAFT_3586 0.51 -0.7
Or see this region's nucleotide sequence