Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3495 and EX28DRAFT_3496 are separated by 41 nucleotides EX28DRAFT_3496 and EX28DRAFT_3497 are separated by 87 nucleotides EX28DRAFT_3497 and EX28DRAFT_3498 are separated by 125 nucleotides EX28DRAFT_3498 and EX28DRAFT_3499 are separated by 30 nucleotides
EX28DRAFT_3495: EX28DRAFT_3495 - sugar (Glycoside-Pentoside-Hexuronide) transporter, at 158,023 to 159,417
_3495
EX28DRAFT_3496: EX28DRAFT_3496 - Alpha/beta hydrolase family, at 159,459 to 159,659
_3496
EX28DRAFT_3497: EX28DRAFT_3497 - hypothetical protein, at 159,747 to 160,988
_3497
EX28DRAFT_3498: EX28DRAFT_3498 - Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily), at 161,114 to 161,935
_3498
EX28DRAFT_3499: EX28DRAFT_3499 - Arabinose efflux permease, at 161,966 to 163,585
_3499
Position (kb) 159
160
161 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 158.779 kb on - strand, within EX28DRAFT_3495 at 158.907 kb on + strand, within EX28DRAFT_3495 at 158.908 kb on - strand, within EX28DRAFT_3495 at 158.908 kb on - strand, within EX28DRAFT_3495 at 158.985 kb on + strand, within EX28DRAFT_3495 at 159.073 kb on + strand, within EX28DRAFT_3495 at 159.073 kb on + strand, within EX28DRAFT_3495 at 159.288 kb on + strand at 159.300 kb on + strand at 159.416 kb on - strand at 159.424 kb on + strand at 159.712 kb on - strand at 159.716 kb on - strand at 159.771 kb on + strand at 159.775 kb on - strand at 159.826 kb on - strand at 159.846 kb on + strand at 159.984 kb on - strand, within EX28DRAFT_3497 at 160.044 kb on - strand, within EX28DRAFT_3497 at 160.055 kb on - strand, within EX28DRAFT_3497 at 160.111 kb on - strand, within EX28DRAFT_3497 at 160.241 kb on - strand, within EX28DRAFT_3497 at 160.364 kb on + strand, within EX28DRAFT_3497 at 160.485 kb on + strand, within EX28DRAFT_3497 at 160.511 kb on + strand, within EX28DRAFT_3497 at 160.511 kb on + strand, within EX28DRAFT_3497 at 160.512 kb on - strand, within EX28DRAFT_3497 at 160.512 kb on - strand, within EX28DRAFT_3497 at 160.545 kb on + strand, within EX28DRAFT_3497 at 160.569 kb on + strand, within EX28DRAFT_3497 at 160.570 kb on - strand, within EX28DRAFT_3497 at 160.791 kb on + strand, within EX28DRAFT_3497 at 160.885 kb on + strand at 160.896 kb on - strand at 160.991 kb on + strand at 160.991 kb on + strand at 160.992 kb on - strand at 160.992 kb on - strand at 160.992 kb on - strand at 161.022 kb on + strand at 161.022 kb on + strand at 161.045 kb on + strand at 161.045 kb on + strand at 161.046 kb on - strand at 161.116 kb on - strand at 161.783 kb on + strand, within EX28DRAFT_3498 at 161.812 kb on - strand, within EX28DRAFT_3498 at 161.812 kb on - strand, within EX28DRAFT_3498 at 161.812 kb on - strand, within EX28DRAFT_3498 at 161.895 kb on + strand at 161.899 kb on - strand at 161.935 kb on + strand at 161.935 kb on + strand at 161.936 kb on - strand at 161.936 kb on - strand at 161.936 kb on - strand at 161.936 kb on - strand at 161.967 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 158,779 - EX28DRAFT_3495 0.54 -0.2 158,907 + EX28DRAFT_3495 0.63 +0.7 158,908 - EX28DRAFT_3495 0.63 +0.1 158,908 - EX28DRAFT_3495 0.63 -0.1 158,985 + EX28DRAFT_3495 0.69 -0.2 159,073 + EX28DRAFT_3495 0.75 +0.5 159,073 + EX28DRAFT_3495 0.75 -0.6 159,288 + -0.2 159,300 + +0.3 159,416 - +1.2 159,424 + -0.5 159,712 - -1.2 159,716 - -1.5 159,771 + -0.7 159,775 - -2.3 159,826 - +0.4 159,846 + -0.2 159,984 - EX28DRAFT_3497 0.19 -0.1 160,044 - EX28DRAFT_3497 0.24 -0.2 160,055 - EX28DRAFT_3497 0.25 +0.3 160,111 - EX28DRAFT_3497 0.29 +0.8 160,241 - EX28DRAFT_3497 0.40 +0.0 160,364 + EX28DRAFT_3497 0.50 +0.4 160,485 + EX28DRAFT_3497 0.59 -0.1 160,511 + EX28DRAFT_3497 0.62 +0.4 160,511 + EX28DRAFT_3497 0.62 +0.4 160,512 - EX28DRAFT_3497 0.62 +0.2 160,512 - EX28DRAFT_3497 0.62 +1.1 160,545 + EX28DRAFT_3497 0.64 +0.8 160,569 + EX28DRAFT_3497 0.66 +0.7 160,570 - EX28DRAFT_3497 0.66 +0.7 160,791 + EX28DRAFT_3497 0.84 +0.1 160,885 + +0.2 160,896 - -0.1 160,991 + +0.1 160,991 + +0.2 160,992 - -1.8 160,992 - -0.6 160,992 - -0.1 161,022 + -0.4 161,022 + +0.4 161,045 + +0.3 161,045 + -2.0 161,046 - +0.8 161,116 - -1.4 161,783 + EX28DRAFT_3498 0.81 +0.8 161,812 - EX28DRAFT_3498 0.85 +0.1 161,812 - EX28DRAFT_3498 0.85 +3.3 161,812 - EX28DRAFT_3498 0.85 +0.7 161,895 + -0.3 161,899 - -0.0 161,935 + -0.0 161,935 + -0.1 161,936 - +0.3 161,936 - -0.1 161,936 - -0.1 161,936 - +1.1 161,967 + -0.4
Or see this region's nucleotide sequence