Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3451 and EX28DRAFT_3452 are separated by 10 nucleotides EX28DRAFT_3452 and EX28DRAFT_3453 are separated by 675 nucleotides
EX28DRAFT_3451: EX28DRAFT_3451 - carbamate kinase, at 123,248 to 124,159
_3451
EX28DRAFT_3452: EX28DRAFT_3452 - arginine deiminase (EC 3.5.3.6), at 124,170 to 125,390
_3452
EX28DRAFT_3453: EX28DRAFT_3453 - YhcH/YjgK/YiaL family protein, at 126,066 to 126,518
_3453
Position (kb) 124
125
126 Strain fitness (log2 ratio) -2
-1
0
1
2 at 123.200 kb on + strand at 123.200 kb on + strand at 123.200 kb on + strand at 123.200 kb on + strand at 123.200 kb on + strand at 123.200 kb on + strand at 123.702 kb on + strand, within EX28DRAFT_3451 at 123.702 kb on + strand, within EX28DRAFT_3451 at 123.702 kb on + strand, within EX28DRAFT_3451 at 123.768 kb on + strand, within EX28DRAFT_3451 at 123.768 kb on + strand, within EX28DRAFT_3451 at 123.783 kb on + strand, within EX28DRAFT_3451 at 123.783 kb on + strand, within EX28DRAFT_3451 at 123.783 kb on + strand, within EX28DRAFT_3451 at 123.894 kb on + strand, within EX28DRAFT_3451 at 124.731 kb on + strand, within EX28DRAFT_3452 at 124.746 kb on + strand, within EX28DRAFT_3452 at 124.769 kb on + strand, within EX28DRAFT_3452 at 124.929 kb on + strand, within EX28DRAFT_3452 at 124.988 kb on + strand, within EX28DRAFT_3452 at 125.378 kb on - strand at 125.597 kb on - strand at 125.866 kb on + strand at 125.916 kb on + strand at 125.916 kb on + strand at 125.917 kb on - strand at 125.917 kb on - strand at 126.202 kb on - strand, within EX28DRAFT_3453
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 123,200 + -0.1 123,200 + -2.0 123,200 + +0.3 123,200 + +0.0 123,200 + -0.4 123,200 + -0.1 123,702 + EX28DRAFT_3451 0.50 -0.1 123,702 + EX28DRAFT_3451 0.50 -0.3 123,702 + EX28DRAFT_3451 0.50 -0.5 123,768 + EX28DRAFT_3451 0.57 +0.3 123,768 + EX28DRAFT_3451 0.57 -0.0 123,783 + EX28DRAFT_3451 0.59 -1.0 123,783 + EX28DRAFT_3451 0.59 -0.5 123,783 + EX28DRAFT_3451 0.59 +0.0 123,894 + EX28DRAFT_3451 0.71 +0.7 124,731 + EX28DRAFT_3452 0.46 -1.3 124,746 + EX28DRAFT_3452 0.47 +1.0 124,769 + EX28DRAFT_3452 0.49 -0.2 124,929 + EX28DRAFT_3452 0.62 +1.0 124,988 + EX28DRAFT_3452 0.67 +1.0 125,378 - -0.2 125,597 - -0.3 125,866 + -0.9 125,916 + -1.1 125,916 + +2.3 125,917 - -0.5 125,917 - -0.0 126,202 - EX28DRAFT_3453 0.30 +0.1
Or see this region's nucleotide sequence