Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3319 and EX28DRAFT_3320 are separated by 303 nucleotides EX28DRAFT_3320 and EX28DRAFT_3321 are separated by 473 nucleotides
EX28DRAFT_3319: EX28DRAFT_3319 - The (Largely Gram-negative Bacterial) Hydrophobe/Amphiphile Efflux-1 (HAE1) Family, at 619,372 to 622,485
_3319
EX28DRAFT_3320: EX28DRAFT_3320 - hypothetical protein, at 622,789 to 623,010
_3320
EX28DRAFT_3321: EX28DRAFT_3321 - ABC-type amino acid transport/signal transduction systems, periplasmic component/domain, at 623,484 to 624,509
_3321
Position (kb) 622
623
624 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 621.800 kb on - strand, within EX28DRAFT_3319 at 621.800 kb on - strand, within EX28DRAFT_3319 at 621.800 kb on - strand, within EX28DRAFT_3319 at 621.823 kb on + strand, within EX28DRAFT_3319 at 621.823 kb on + strand, within EX28DRAFT_3319 at 621.877 kb on - strand, within EX28DRAFT_3319 at 621.895 kb on - strand, within EX28DRAFT_3319 at 621.997 kb on + strand, within EX28DRAFT_3319 at 621.998 kb on - strand, within EX28DRAFT_3319 at 622.042 kb on + strand, within EX28DRAFT_3319 at 622.145 kb on - strand, within EX28DRAFT_3319 at 622.216 kb on - strand at 622.484 kb on - strand at 622.525 kb on + strand at 622.572 kb on - strand at 622.572 kb on - strand at 622.586 kb on + strand at 622.586 kb on + strand at 622.605 kb on - strand at 622.607 kb on - strand at 622.607 kb on - strand at 622.615 kb on - strand at 622.669 kb on + strand at 622.674 kb on - strand at 622.771 kb on + strand at 622.860 kb on - strand, within EX28DRAFT_3320 at 623.094 kb on + strand at 623.095 kb on - strand at 623.096 kb on + strand at 623.097 kb on - strand at 623.097 kb on - strand at 623.193 kb on + strand at 623.231 kb on - strand at 623.323 kb on + strand at 623.333 kb on + strand at 623.334 kb on - strand at 623.390 kb on + strand at 623.403 kb on + strand at 623.430 kb on + strand at 623.431 kb on - strand at 623.451 kb on + strand at 623.452 kb on - strand at 623.452 kb on - strand at 623.483 kb on + strand at 623.632 kb on - strand, within EX28DRAFT_3321 at 623.720 kb on - strand, within EX28DRAFT_3321 at 623.720 kb on - strand, within EX28DRAFT_3321 at 623.724 kb on + strand, within EX28DRAFT_3321 at 623.725 kb on - strand, within EX28DRAFT_3321 at 623.725 kb on - strand, within EX28DRAFT_3321 at 623.854 kb on - strand, within EX28DRAFT_3321 at 623.854 kb on - strand, within EX28DRAFT_3321 at 623.854 kb on - strand, within EX28DRAFT_3321 at 623.857 kb on - strand, within EX28DRAFT_3321 at 623.867 kb on - strand, within EX28DRAFT_3321 at 623.934 kb on - strand, within EX28DRAFT_3321 at 623.974 kb on - strand, within EX28DRAFT_3321 at 623.997 kb on + strand, within EX28DRAFT_3321 at 623.997 kb on + strand, within EX28DRAFT_3321 at 623.998 kb on - strand, within EX28DRAFT_3321 at 623.998 kb on - strand, within EX28DRAFT_3321
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 621,800 - EX28DRAFT_3319 0.78 -0.1 621,800 - EX28DRAFT_3319 0.78 -1.3 621,800 - EX28DRAFT_3319 0.78 +0.2 621,823 + EX28DRAFT_3319 0.79 -0.0 621,823 + EX28DRAFT_3319 0.79 +0.8 621,877 - EX28DRAFT_3319 0.80 +0.2 621,895 - EX28DRAFT_3319 0.81 -0.9 621,997 + EX28DRAFT_3319 0.84 +0.7 621,998 - EX28DRAFT_3319 0.84 +0.7 622,042 + EX28DRAFT_3319 0.86 -1.2 622,145 - EX28DRAFT_3319 0.89 +0.0 622,216 - -0.2 622,484 - -3.1 622,525 + +0.2 622,572 - -0.1 622,572 - -1.3 622,586 + -0.9 622,586 + +0.7 622,605 - +0.7 622,607 - +0.1 622,607 - +0.0 622,615 - +0.3 622,669 + -0.2 622,674 - +0.3 622,771 + +0.2 622,860 - EX28DRAFT_3320 0.32 -1.9 623,094 + -0.3 623,095 - +1.0 623,096 + -0.1 623,097 - +0.4 623,097 - +0.3 623,193 + +0.8 623,231 - +0.3 623,323 + +0.7 623,333 + -0.6 623,334 - +0.5 623,390 + -0.3 623,403 + -0.6 623,430 + +0.5 623,431 - +0.1 623,451 + +1.7 623,452 - +0.8 623,452 - -0.1 623,483 + -0.7 623,632 - EX28DRAFT_3321 0.14 -2.9 623,720 - EX28DRAFT_3321 0.23 +0.1 623,720 - EX28DRAFT_3321 0.23 +0.0 623,724 + EX28DRAFT_3321 0.23 -0.4 623,725 - EX28DRAFT_3321 0.23 +0.1 623,725 - EX28DRAFT_3321 0.23 -0.5 623,854 - EX28DRAFT_3321 0.36 +0.0 623,854 - EX28DRAFT_3321 0.36 +1.3 623,854 - EX28DRAFT_3321 0.36 -0.7 623,857 - EX28DRAFT_3321 0.36 +0.9 623,867 - EX28DRAFT_3321 0.37 +0.7 623,934 - EX28DRAFT_3321 0.44 -1.1 623,974 - EX28DRAFT_3321 0.48 +0.5 623,997 + EX28DRAFT_3321 0.50 +0.4 623,997 + EX28DRAFT_3321 0.50 +0.5 623,998 - EX28DRAFT_3321 0.50 -0.8 623,998 - EX28DRAFT_3321 0.50 -0.8
Or see this region's nucleotide sequence