Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2946 and EX28DRAFT_2947 are separated by 6 nucleotides EX28DRAFT_2947 and EX28DRAFT_2948 overlap by 20 nucleotides EX28DRAFT_2948 and EX28DRAFT_2949 are separated by 294 nucleotides
EX28DRAFT_2946: EX28DRAFT_2946 - flavodoxin, long chain, at 257,006 to 257,527
_2946
EX28DRAFT_2947: EX28DRAFT_2947 - Protein of unknown function (DUF1434), at 257,534 to 257,944
_2947
EX28DRAFT_2948: EX28DRAFT_2948 - Uncharacterized conserved protein, at 257,925 to 258,191
_2948
EX28DRAFT_2949: EX28DRAFT_2949 - folate-binding protein YgfZ, at 258,486 to 259,466
_2949
Position (kb) 257
258
259 Strain fitness (log2 ratio) -2
-1
0
1
2 at 256.936 kb on + strand at 256.996 kb on + strand at 257.001 kb on - strand at 257.011 kb on + strand at 257.012 kb on - strand at 257.025 kb on - strand at 257.141 kb on + strand, within EX28DRAFT_2946 at 257.142 kb on - strand, within EX28DRAFT_2946 at 257.187 kb on + strand, within EX28DRAFT_2946 at 257.206 kb on + strand, within EX28DRAFT_2946 at 257.206 kb on + strand, within EX28DRAFT_2946 at 257.357 kb on + strand, within EX28DRAFT_2946 at 257.597 kb on - strand, within EX28DRAFT_2947 at 257.738 kb on + strand, within EX28DRAFT_2947 at 257.744 kb on - strand, within EX28DRAFT_2947 at 257.830 kb on + strand, within EX28DRAFT_2947 at 257.831 kb on - strand, within EX28DRAFT_2947 at 257.831 kb on - strand, within EX28DRAFT_2947 at 257.831 kb on - strand, within EX28DRAFT_2947 at 257.831 kb on - strand, within EX28DRAFT_2947 at 257.833 kb on - strand, within EX28DRAFT_2947 at 257.833 kb on - strand, within EX28DRAFT_2947 at 257.888 kb on + strand, within EX28DRAFT_2947 at 257.967 kb on + strand, within EX28DRAFT_2948 at 258.097 kb on + strand, within EX28DRAFT_2948 at 258.097 kb on + strand, within EX28DRAFT_2948 at 258.098 kb on - strand, within EX28DRAFT_2948 at 258.098 kb on - strand, within EX28DRAFT_2948 at 258.098 kb on - strand, within EX28DRAFT_2948 at 258.098 kb on - strand, within EX28DRAFT_2948 at 258.103 kb on + strand, within EX28DRAFT_2948 at 258.104 kb on - strand, within EX28DRAFT_2948 at 258.128 kb on + strand, within EX28DRAFT_2948 at 258.208 kb on + strand at 258.208 kb on + strand at 258.209 kb on - strand at 258.209 kb on - strand at 258.245 kb on + strand at 258.246 kb on - strand at 258.269 kb on - strand at 258.269 kb on - strand at 258.273 kb on + strand at 258.305 kb on - strand at 258.334 kb on - strand at 258.361 kb on + strand at 258.441 kb on + strand at 258.441 kb on + strand at 258.441 kb on + strand at 258.447 kb on + strand at 258.454 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 256,936 + -0.5 256,996 + +0.2 257,001 - +0.5 257,011 + -0.8 257,012 - -0.1 257,025 - -0.5 257,141 + EX28DRAFT_2946 0.26 +0.3 257,142 - EX28DRAFT_2946 0.26 -0.7 257,187 + EX28DRAFT_2946 0.35 -0.6 257,206 + EX28DRAFT_2946 0.38 +0.5 257,206 + EX28DRAFT_2946 0.38 -0.6 257,357 + EX28DRAFT_2946 0.67 +1.4 257,597 - EX28DRAFT_2947 0.15 -0.6 257,738 + EX28DRAFT_2947 0.50 -0.1 257,744 - EX28DRAFT_2947 0.51 +1.0 257,830 + EX28DRAFT_2947 0.72 +0.1 257,831 - EX28DRAFT_2947 0.72 +0.7 257,831 - EX28DRAFT_2947 0.72 -0.0 257,831 - EX28DRAFT_2947 0.72 +0.4 257,831 - EX28DRAFT_2947 0.72 +0.4 257,833 - EX28DRAFT_2947 0.73 -0.9 257,833 - EX28DRAFT_2947 0.73 +0.2 257,888 + EX28DRAFT_2947 0.86 -0.6 257,967 + EX28DRAFT_2948 0.16 -2.7 258,097 + EX28DRAFT_2948 0.64 -1.2 258,097 + EX28DRAFT_2948 0.64 -1.1 258,098 - EX28DRAFT_2948 0.65 -1.2 258,098 - EX28DRAFT_2948 0.65 -1.0 258,098 - EX28DRAFT_2948 0.65 -0.9 258,098 - EX28DRAFT_2948 0.65 -1.0 258,103 + EX28DRAFT_2948 0.67 -2.1 258,104 - EX28DRAFT_2948 0.67 -1.3 258,128 + EX28DRAFT_2948 0.76 -2.2 258,208 + -0.5 258,208 + -1.5 258,209 - -2.5 258,209 - +0.6 258,245 + -0.2 258,246 - +2.4 258,269 - +0.3 258,269 - +0.1 258,273 + +0.3 258,305 - -1.7 258,334 - -0.0 258,361 + -0.4 258,441 + -1.2 258,441 + -0.1 258,441 + -0.0 258,447 + +0.4 258,454 + -0.0
Or see this region's nucleotide sequence