Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2812 and EX28DRAFT_2813 are separated by 15 nucleotides EX28DRAFT_2813 and EX28DRAFT_2814 are separated by 1 nucleotides EX28DRAFT_2814 and EX28DRAFT_2815 overlap by 4 nucleotides EX28DRAFT_2815 and EX28DRAFT_2816 are separated by 69 nucleotides
EX28DRAFT_2812: EX28DRAFT_2812 - Ni,Fe-hydrogenase III large subunit, at 114,997 to 116,706
_2812
EX28DRAFT_2813: EX28DRAFT_2813 - Formate hydrogenlyase subunit 4, at 116,722 to 117,645
_2813
EX28DRAFT_2814: EX28DRAFT_2814 - Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit, at 117,647 to 119,461
_2814
EX28DRAFT_2815: EX28DRAFT_2815 - Fe-S-cluster-containing hydrogenase components 2, at 119,458 to 120,066
_2815
EX28DRAFT_2816: EX28DRAFT_2816 - Transcriptional repressor of hyc and hyp operons, at 120,136 to 120,600
_2816
Position (kb) 117
118
119
120 Strain fitness (log2 ratio) -1
0
1 at 116.678 kb on + strand at 116.706 kb on + strand at 116.706 kb on + strand at 116.707 kb on - strand at 116.707 kb on - strand at 116.710 kb on + strand at 116.710 kb on + strand at 116.710 kb on + strand at 116.711 kb on - strand at 116.711 kb on - strand at 116.711 kb on - strand at 116.720 kb on - strand at 116.749 kb on + strand at 117.341 kb on + strand, within EX28DRAFT_2813 at 117.342 kb on - strand, within EX28DRAFT_2813 at 117.345 kb on + strand, within EX28DRAFT_2813 at 117.345 kb on + strand, within EX28DRAFT_2813 at 117.346 kb on - strand, within EX28DRAFT_2813 at 117.988 kb on + strand, within EX28DRAFT_2814 at 118.291 kb on - strand, within EX28DRAFT_2814 at 118.291 kb on - strand, within EX28DRAFT_2814 at 118.469 kb on - strand, within EX28DRAFT_2814 at 119.192 kb on - strand, within EX28DRAFT_2814 at 120.085 kb on + strand at 120.086 kb on - strand at 120.212 kb on + strand, within EX28DRAFT_2816 at 120.224 kb on + strand, within EX28DRAFT_2816 at 120.225 kb on - strand, within EX28DRAFT_2816 at 120.249 kb on - strand, within EX28DRAFT_2816 at 120.330 kb on + strand, within EX28DRAFT_2816 at 120.330 kb on + strand, within EX28DRAFT_2816
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 116,678 + -0.3 116,706 + -0.3 116,706 + -0.0 116,707 - +0.3 116,707 - -0.5 116,710 + +0.5 116,710 + -0.5 116,710 + +0.3 116,711 - -0.4 116,711 - +0.4 116,711 - -0.9 116,720 - +0.5 116,749 + +0.6 117,341 + EX28DRAFT_2813 0.67 +0.5 117,342 - EX28DRAFT_2813 0.67 -1.1 117,345 + EX28DRAFT_2813 0.67 +0.4 117,345 + EX28DRAFT_2813 0.67 -1.5 117,346 - EX28DRAFT_2813 0.68 +0.5 117,988 + EX28DRAFT_2814 0.19 +0.0 118,291 - EX28DRAFT_2814 0.35 -0.7 118,291 - EX28DRAFT_2814 0.35 +0.0 118,469 - EX28DRAFT_2814 0.45 +0.3 119,192 - EX28DRAFT_2814 0.85 +0.3 120,085 + +1.4 120,086 - +0.2 120,212 + EX28DRAFT_2816 0.16 -0.7 120,224 + EX28DRAFT_2816 0.19 +1.3 120,225 - EX28DRAFT_2816 0.19 -0.3 120,249 - EX28DRAFT_2816 0.24 +0.3 120,330 + EX28DRAFT_2816 0.42 -0.8 120,330 + EX28DRAFT_2816 0.42 -0.7
Or see this region's nucleotide sequence