Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2543 and EX28DRAFT_2544 are separated by 148 nucleotides EX28DRAFT_2544 and EX28DRAFT_2545 are separated by 32 nucleotides EX28DRAFT_2545 and EX28DRAFT_2546 are separated by 121 nucleotides
EX28DRAFT_2543: EX28DRAFT_2543 - protoheme IX farnesyltransferase, at 2,575,529 to 2,576,416
_2543
EX28DRAFT_2544: EX28DRAFT_2544 - Arabinose efflux permease, at 2,576,565 to 2,577,926
_2544
EX28DRAFT_2545: EX28DRAFT_2545 - Uncharacterized protein conserved in bacteria, at 2,577,959 to 2,578,450
_2545
EX28DRAFT_2546: EX28DRAFT_2546 - 2-dehydropantoate 2-reductase, at 2,578,572 to 2,579,483
_2546
Position (kb)
2576
2577
2578 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2575.642 kb on + strand, within EX28DRAFT_2543 at 2575.646 kb on + strand, within EX28DRAFT_2543 at 2575.647 kb on - strand, within EX28DRAFT_2543 at 2575.700 kb on + strand, within EX28DRAFT_2543 at 2575.701 kb on - strand, within EX28DRAFT_2543 at 2575.883 kb on + strand, within EX28DRAFT_2543 at 2575.883 kb on + strand, within EX28DRAFT_2543 at 2575.904 kb on + strand, within EX28DRAFT_2543 at 2575.904 kb on + strand, within EX28DRAFT_2543 at 2575.905 kb on - strand, within EX28DRAFT_2543 at 2576.222 kb on + strand, within EX28DRAFT_2543 at 2576.405 kb on + strand at 2576.406 kb on - strand at 2576.431 kb on + strand at 2576.431 kb on + strand at 2576.476 kb on + strand at 2576.477 kb on - strand at 2576.488 kb on + strand at 2576.489 kb on - strand at 2576.533 kb on - strand at 2576.538 kb on - strand at 2576.564 kb on - strand at 2576.575 kb on - strand at 2576.614 kb on + strand at 2576.677 kb on - strand at 2576.734 kb on - strand, within EX28DRAFT_2544 at 2577.038 kb on + strand, within EX28DRAFT_2544 at 2577.039 kb on - strand, within EX28DRAFT_2544 at 2577.166 kb on + strand, within EX28DRAFT_2544 at 2577.166 kb on + strand, within EX28DRAFT_2544 at 2577.167 kb on - strand, within EX28DRAFT_2544 at 2577.228 kb on - strand, within EX28DRAFT_2544 at 2577.591 kb on + strand, within EX28DRAFT_2544 at 2577.592 kb on - strand, within EX28DRAFT_2544 at 2577.741 kb on + strand, within EX28DRAFT_2544 at 2577.797 kb on + strand at 2577.798 kb on - strand at 2577.874 kb on + strand at 2577.874 kb on + strand at 2578.093 kb on + strand, within EX28DRAFT_2545 at 2578.093 kb on + strand, within EX28DRAFT_2545 at 2578.109 kb on - strand, within EX28DRAFT_2545 at 2578.109 kb on - strand, within EX28DRAFT_2545 at 2578.111 kb on + strand, within EX28DRAFT_2545 at 2578.111 kb on + strand, within EX28DRAFT_2545 at 2578.111 kb on + strand, within EX28DRAFT_2545 at 2578.112 kb on - strand, within EX28DRAFT_2545 at 2578.112 kb on - strand, within EX28DRAFT_2545 at 2578.307 kb on - strand, within EX28DRAFT_2545 at 2578.484 kb on + strand at 2578.519 kb on + strand at 2578.525 kb on + strand at 2578.526 kb on - strand at 2578.527 kb on + strand at 2578.528 kb on - strand at 2578.531 kb on + strand at 2578.531 kb on + strand at 2578.532 kb on - strand at 2578.678 kb on - strand, within EX28DRAFT_2546 at 2578.772 kb on - strand, within EX28DRAFT_2546
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 2,575,642 + EX28DRAFT_2543 0.13 -1.7 2,575,646 + EX28DRAFT_2543 0.13 -1.1 2,575,647 - EX28DRAFT_2543 0.13 -0.2 2,575,700 + EX28DRAFT_2543 0.19 +1.3 2,575,701 - EX28DRAFT_2543 0.19 -0.6 2,575,883 + EX28DRAFT_2543 0.40 -0.8 2,575,883 + EX28DRAFT_2543 0.40 -1.1 2,575,904 + EX28DRAFT_2543 0.42 -3.0 2,575,904 + EX28DRAFT_2543 0.42 -1.1 2,575,905 - EX28DRAFT_2543 0.42 +0.3 2,576,222 + EX28DRAFT_2543 0.78 -0.9 2,576,405 + -0.7 2,576,406 - +0.3 2,576,431 + -0.8 2,576,431 + -0.2 2,576,476 + -1.5 2,576,477 - +0.5 2,576,488 + -0.0 2,576,489 - -0.3 2,576,533 - +0.5 2,576,538 - +1.6 2,576,564 - -0.7 2,576,575 - -0.2 2,576,614 + -2.0 2,576,677 - -1.1 2,576,734 - EX28DRAFT_2544 0.12 -0.8 2,577,038 + EX28DRAFT_2544 0.35 -1.1 2,577,039 - EX28DRAFT_2544 0.35 -0.7 2,577,166 + EX28DRAFT_2544 0.44 +2.0 2,577,166 + EX28DRAFT_2544 0.44 -2.5 2,577,167 - EX28DRAFT_2544 0.44 -1.1 2,577,228 - EX28DRAFT_2544 0.49 -1.0 2,577,591 + EX28DRAFT_2544 0.75 -1.0 2,577,592 - EX28DRAFT_2544 0.75 -2.1 2,577,741 + EX28DRAFT_2544 0.86 +0.7 2,577,797 + -0.6 2,577,798 - -1.9 2,577,874 + -1.1 2,577,874 + +0.5 2,578,093 + EX28DRAFT_2545 0.27 +0.4 2,578,093 + EX28DRAFT_2545 0.27 +0.5 2,578,109 - EX28DRAFT_2545 0.30 +0.0 2,578,109 - EX28DRAFT_2545 0.30 +1.0 2,578,111 + EX28DRAFT_2545 0.31 +2.2 2,578,111 + EX28DRAFT_2545 0.31 +1.0 2,578,111 + EX28DRAFT_2545 0.31 +0.5 2,578,112 - EX28DRAFT_2545 0.31 -0.2 2,578,112 - EX28DRAFT_2545 0.31 -0.4 2,578,307 - EX28DRAFT_2545 0.71 -0.1 2,578,484 + -1.5 2,578,519 + -0.5 2,578,525 + +0.1 2,578,526 - -0.2 2,578,527 + -1.5 2,578,528 - -0.2 2,578,531 + -0.1 2,578,531 + -0.5 2,578,532 - -0.2 2,578,678 - EX28DRAFT_2546 0.12 -1.1 2,578,772 - EX28DRAFT_2546 0.22 -0.4
Or see this region's nucleotide sequence