Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2457 and EX28DRAFT_2458 are separated by 68 nucleotides EX28DRAFT_2458 and EX28DRAFT_2459 are separated by 161 nucleotides EX28DRAFT_2459 and EX28DRAFT_2460 overlap by 4 nucleotides EX28DRAFT_2460 and EX28DRAFT_2461 are separated by 84 nucleotides
EX28DRAFT_2457: EX28DRAFT_2457 - Protein of unknown function (DUF1471), at 2,487,499 to 2,487,708
_2457
EX28DRAFT_2458: EX28DRAFT_2458 - hypothetical protein, at 2,487,777 to 2,487,962
_2458
EX28DRAFT_2459: EX28DRAFT_2459 - phosphoribosylaminoimidazole carboxylase, PurE protein, at 2,488,124 to 2,488,633
_2459
EX28DRAFT_2460: EX28DRAFT_2460 - phosphoribosylaminoimidazole carboxylase, PurK protein, at 2,488,630 to 2,489,697
_2460
EX28DRAFT_2461: EX28DRAFT_2461 - tRNA 2-selenouridine synthase, at 2,489,782 to 2,490,852
_2461
Position (kb)
2488
2489
2490 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2487.647 kb on - strand, within EX28DRAFT_2457 at 2487.648 kb on + strand, within EX28DRAFT_2457 at 2487.649 kb on - strand, within EX28DRAFT_2457 at 2487.682 kb on + strand, within EX28DRAFT_2457 at 2487.682 kb on + strand, within EX28DRAFT_2457 at 2487.683 kb on - strand, within EX28DRAFT_2457 at 2487.707 kb on - strand at 2487.735 kb on - strand at 2487.755 kb on + strand at 2487.796 kb on + strand, within EX28DRAFT_2458 at 2487.796 kb on + strand, within EX28DRAFT_2458 at 2487.796 kb on + strand, within EX28DRAFT_2458 at 2487.796 kb on + strand, within EX28DRAFT_2458 at 2487.797 kb on - strand, within EX28DRAFT_2458 at 2487.801 kb on - strand, within EX28DRAFT_2458 at 2487.960 kb on + strand at 2487.970 kb on + strand at 2487.970 kb on + strand at 2488.018 kb on - strand at 2488.061 kb on + strand at 2488.555 kb on - strand, within EX28DRAFT_2459 at 2488.664 kb on + strand at 2489.254 kb on - strand, within EX28DRAFT_2460 at 2489.284 kb on + strand, within EX28DRAFT_2460 at 2489.569 kb on - strand, within EX28DRAFT_2460 at 2489.695 kb on + strand at 2489.696 kb on - strand at 2489.738 kb on + strand at 2489.738 kb on + strand at 2489.739 kb on - strand at 2489.935 kb on + strand, within EX28DRAFT_2461 at 2489.935 kb on + strand, within EX28DRAFT_2461 at 2489.935 kb on + strand, within EX28DRAFT_2461 at 2490.087 kb on + strand, within EX28DRAFT_2461 at 2490.088 kb on - strand, within EX28DRAFT_2461 at 2490.148 kb on - strand, within EX28DRAFT_2461 at 2490.148 kb on - strand, within EX28DRAFT_2461 at 2490.529 kb on + strand, within EX28DRAFT_2461 at 2490.601 kb on + strand, within EX28DRAFT_2461
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 2,487,647 - EX28DRAFT_2457 0.70 +0.0 2,487,648 + EX28DRAFT_2457 0.71 +0.9 2,487,649 - EX28DRAFT_2457 0.71 -0.0 2,487,682 + EX28DRAFT_2457 0.87 +0.1 2,487,682 + EX28DRAFT_2457 0.87 +0.2 2,487,683 - EX28DRAFT_2457 0.88 -0.1 2,487,707 - +0.4 2,487,735 - -0.0 2,487,755 + +1.7 2,487,796 + EX28DRAFT_2458 0.10 -0.4 2,487,796 + EX28DRAFT_2458 0.10 +0.3 2,487,796 + EX28DRAFT_2458 0.10 +1.3 2,487,796 + EX28DRAFT_2458 0.10 -0.0 2,487,797 - EX28DRAFT_2458 0.11 +0.9 2,487,801 - EX28DRAFT_2458 0.13 +1.1 2,487,960 + +1.4 2,487,970 + +0.4 2,487,970 + +0.2 2,488,018 - -0.3 2,488,061 + -1.0 2,488,555 - EX28DRAFT_2459 0.85 -2.9 2,488,664 + -2.1 2,489,254 - EX28DRAFT_2460 0.58 -2.7 2,489,284 + EX28DRAFT_2460 0.61 -2.6 2,489,569 - EX28DRAFT_2460 0.88 -1.1 2,489,695 + -0.6 2,489,696 - +0.9 2,489,738 + -0.9 2,489,738 + -1.5 2,489,739 - -0.2 2,489,935 + EX28DRAFT_2461 0.14 +0.5 2,489,935 + EX28DRAFT_2461 0.14 +0.9 2,489,935 + EX28DRAFT_2461 0.14 -0.5 2,490,087 + EX28DRAFT_2461 0.28 +0.7 2,490,088 - EX28DRAFT_2461 0.29 +0.3 2,490,148 - EX28DRAFT_2461 0.34 +1.0 2,490,148 - EX28DRAFT_2461 0.34 +0.3 2,490,529 + EX28DRAFT_2461 0.70 +0.6 2,490,601 + EX28DRAFT_2461 0.76 -0.9
Or see this region's nucleotide sequence